Benjamin E D
Synchronisation of oestrus,Superovulation and Embryo Collection in Goats - Mannuthy Department of Animal Reproduction, College of Veterinary and Animal Sciences 1994
With the object of evolving effective methods for standardizing techniques for synchronization of oestrus superovulation and collection of embryos, 18 healthy goats were selected from the goat farm attached to the College of Veterinary and Animal Sciences, Mannuthy, and randomly divided into three different groups with six animals in each. Animals in the first group were given two doses of 10 mg PG2a 11 days apart and those in the second group were given 12.5 mg progesterone injection daily for 16 days. Six animals in the third group were not given any treatment and kept as control.
The respective treatments were repeated in group I and II, after a period of sixty days and superovulation in group I was carried out with eCG injection 1000 IU given intramuscularly on the day previous to the second dose of PGF2a. In group II, superovulation was done by intramuscular injection of 1000 IU eCG given on the 15th day of progesterone treatment. All the animals in the above groups and animals in the control group were inseminated with good quality buck semen four to six hours after the onset of oestrus. Embryos were collected surgically in all the eighteen goats by flushing the fallopian tube towards the fimbria after conducting laparotomy of the inseminated goats on the left flank.
All the animals in group I came to oestrus 57.8 ± 5.65 h after the second injection of PGF2a and the duration of oestrus was 48 ± 8.76 h.
In group II, 83.33 % were in oestrus at an interval of 101.6 ± 6.11 h after the last progesterone injection and the duration of oestrus was 28 ± 1.41 h.
The results of administration of eCG in the second treatment regime with PGF2a in group I, revealed that all animals in this group evinced oestrus at a mean interval of 50.3 ± 10.86 h after the second injection of PGF2a and the mean duration of oestrus was 44 ± 4h. The total number of ovulation points on both the ovaries in this group were 8.4 ± 1.94 with 4 ± 1.30 and 4.4 ± 0.748 for the right and left ovaries respectively. The total number of unruptured follicles on both the ovaries was 5.33 ± 1.64.
The animals in group II, after administration of eCG and progesterone evinced oestrus 72 ± 9.06 h after the last progesterone injection with the duration of oestrus as 38.3 ± 4.46 h. The total number of ovulation points on both the ovaries was 12.8 ± 1.4 and the values were 7.5 ± 2.31 for the right and 5.3 ± 2.04 for the left ovary. The total number of unruptured follicles on both the ovaries was 3.2 ± 1.579.
The results of embryo collection in animals in group I, revealed that the average number of embryos collected from both the ovaries was 4.8 ± 0.97 with 2.2 ± 0.66 for the right and 2.6 ± 0.39 for the left ovary. The total number of embryos collected from all the animals in both the ovaries was 24 (57.14%) of which 17 (70.83%) were transferable.
The results of embryo collection in animals in group II showed that the average number of embryos collected from both the ovaries was 6.5 ± 2.95 with 3.3 ± 1.57 for the right and 3.2 ± 1.47 for the left ovary. The total number of embryos collected from all the animals in both the ovaries was 39 (50.64%) of which 29 (74.36%) were transferable.
In the control animals, the total number of ovulation points on both the ovaries averaged 0.83 ± 0.166 with a value of 0.5 ± 0.223 for the right and 0.33 ± 0.210 for the left ovary. The percentage of transferable embryos collected was 100%.
It was concluded that for the purpose of synchronization and superovulation, prostaglandin, progesterone and eCG can be effectively used in goats for embryo collection without affecting the future reproductive performance.
636.082 / BEN/SY
Synchronisation of oestrus,Superovulation and Embryo Collection in Goats - Mannuthy Department of Animal Reproduction, College of Veterinary and Animal Sciences 1994
With the object of evolving effective methods for standardizing techniques for synchronization of oestrus superovulation and collection of embryos, 18 healthy goats were selected from the goat farm attached to the College of Veterinary and Animal Sciences, Mannuthy, and randomly divided into three different groups with six animals in each. Animals in the first group were given two doses of 10 mg PG2a 11 days apart and those in the second group were given 12.5 mg progesterone injection daily for 16 days. Six animals in the third group were not given any treatment and kept as control.
The respective treatments were repeated in group I and II, after a period of sixty days and superovulation in group I was carried out with eCG injection 1000 IU given intramuscularly on the day previous to the second dose of PGF2a. In group II, superovulation was done by intramuscular injection of 1000 IU eCG given on the 15th day of progesterone treatment. All the animals in the above groups and animals in the control group were inseminated with good quality buck semen four to six hours after the onset of oestrus. Embryos were collected surgically in all the eighteen goats by flushing the fallopian tube towards the fimbria after conducting laparotomy of the inseminated goats on the left flank.
All the animals in group I came to oestrus 57.8 ± 5.65 h after the second injection of PGF2a and the duration of oestrus was 48 ± 8.76 h.
In group II, 83.33 % were in oestrus at an interval of 101.6 ± 6.11 h after the last progesterone injection and the duration of oestrus was 28 ± 1.41 h.
The results of administration of eCG in the second treatment regime with PGF2a in group I, revealed that all animals in this group evinced oestrus at a mean interval of 50.3 ± 10.86 h after the second injection of PGF2a and the mean duration of oestrus was 44 ± 4h. The total number of ovulation points on both the ovaries in this group were 8.4 ± 1.94 with 4 ± 1.30 and 4.4 ± 0.748 for the right and left ovaries respectively. The total number of unruptured follicles on both the ovaries was 5.33 ± 1.64.
The animals in group II, after administration of eCG and progesterone evinced oestrus 72 ± 9.06 h after the last progesterone injection with the duration of oestrus as 38.3 ± 4.46 h. The total number of ovulation points on both the ovaries was 12.8 ± 1.4 and the values were 7.5 ± 2.31 for the right and 5.3 ± 2.04 for the left ovary. The total number of unruptured follicles on both the ovaries was 3.2 ± 1.579.
The results of embryo collection in animals in group I, revealed that the average number of embryos collected from both the ovaries was 4.8 ± 0.97 with 2.2 ± 0.66 for the right and 2.6 ± 0.39 for the left ovary. The total number of embryos collected from all the animals in both the ovaries was 24 (57.14%) of which 17 (70.83%) were transferable.
The results of embryo collection in animals in group II showed that the average number of embryos collected from both the ovaries was 6.5 ± 2.95 with 3.3 ± 1.57 for the right and 3.2 ± 1.47 for the left ovary. The total number of embryos collected from all the animals in both the ovaries was 39 (50.64%) of which 29 (74.36%) were transferable.
In the control animals, the total number of ovulation points on both the ovaries averaged 0.83 ± 0.166 with a value of 0.5 ± 0.223 for the right and 0.33 ± 0.210 for the left ovary. The percentage of transferable embryos collected was 100%.
It was concluded that for the purpose of synchronization and superovulation, prostaglandin, progesterone and eCG can be effectively used in goats for embryo collection without affecting the future reproductive performance.
636.082 / BEN/SY