Ajith Kumar PV
Standardisation of Media and containers for Ex-Vitro Establishment of Anthurium Plantlets produced by Leaf culture - Vellayani Department of Horticulture, College of Agriculture 1993
Investigations were carried out at the Plant Tissue
Culture Laboratory of the College of Agriculture, Vellayani
during 1991-1993 to develop suitable methods to plant out
Anthurium andreanum plantlets and to standardise media and
containers to maximise the ex vitro establishment and growth
of in vitro derived plantlets.
Segments of leaf were used as explant for reproducing
required number of plantlets for the study. Various factors
influencing in vitro rooting were
standardised.
Plantlets
of 3 cm length with at least three leaves recorded shortest
time (10.24 days) for root initiation and produced maximum
number of roots per shoot compared to smaller shoots.
Combination of BA 0.5 ppm and IAA 2.0 ppm was found to be the
best for in vitro rooting.
Agar at 0.7 % recorded shortest
time (10.54 days) for root initiation and the number of roots
per shoots decreased by increasing its concentration in the
medium, while the length 0f ro0t increased along with
increase in agar concentration.
Sucrose level maintained at
normal level in MS medium (3.0 per cent) was found to be the
best for in vitro rooting.
In order to standardise the media and containers
for ex vitro establishment, media such as coarse sand,
finesand, charcoal, soilrite and sphagnum moss and containers
such as mud pot, plastic pot, paper pot, polythene cover and
netted pot were used.
Plantlets with at least 2.5-3 cm size (with 3-4
leaves and two or more roots) recorded 90.0 to 100.0 per cent
survival irrespective of media and containers. Of the
various madia and containers tried plastic pot as the
container and soilrite as the media recorded highest number
of leaves in the transplanted plants at fortnightly
intervals.
Both one and two months after transplanting, mud
pot outdid other containers and soilrite outdid other media
with respect to plant height. In the case of leaf area at
second and fourth fortnight, plastic pot and at third
fortnight polythene cover was found to be the best container,
and soilrite was the best medium at third fortnight onwards.
The containers showed no significant influence on petiole
length at second and fourth fortnight but at second fortnight
onwards medium soilrite recorded maximum petiole length. The
plants grown in polythene cover with media soilrite recorded
inaximum number of roots and length of roots at two months
after transplanting.
So it is evident that among the media,
soilrite was the best for ex vitro establishment of anthurium
plantlets but containers showed no uniform response with
various growth factors.
635 / AJI/ST
Standardisation of Media and containers for Ex-Vitro Establishment of Anthurium Plantlets produced by Leaf culture - Vellayani Department of Horticulture, College of Agriculture 1993
Investigations were carried out at the Plant Tissue
Culture Laboratory of the College of Agriculture, Vellayani
during 1991-1993 to develop suitable methods to plant out
Anthurium andreanum plantlets and to standardise media and
containers to maximise the ex vitro establishment and growth
of in vitro derived plantlets.
Segments of leaf were used as explant for reproducing
required number of plantlets for the study. Various factors
influencing in vitro rooting were
standardised.
Plantlets
of 3 cm length with at least three leaves recorded shortest
time (10.24 days) for root initiation and produced maximum
number of roots per shoot compared to smaller shoots.
Combination of BA 0.5 ppm and IAA 2.0 ppm was found to be the
best for in vitro rooting.
Agar at 0.7 % recorded shortest
time (10.54 days) for root initiation and the number of roots
per shoots decreased by increasing its concentration in the
medium, while the length 0f ro0t increased along with
increase in agar concentration.
Sucrose level maintained at
normal level in MS medium (3.0 per cent) was found to be the
best for in vitro rooting.
In order to standardise the media and containers
for ex vitro establishment, media such as coarse sand,
finesand, charcoal, soilrite and sphagnum moss and containers
such as mud pot, plastic pot, paper pot, polythene cover and
netted pot were used.
Plantlets with at least 2.5-3 cm size (with 3-4
leaves and two or more roots) recorded 90.0 to 100.0 per cent
survival irrespective of media and containers. Of the
various madia and containers tried plastic pot as the
container and soilrite as the media recorded highest number
of leaves in the transplanted plants at fortnightly
intervals.
Both one and two months after transplanting, mud
pot outdid other containers and soilrite outdid other media
with respect to plant height. In the case of leaf area at
second and fourth fortnight, plastic pot and at third
fortnight polythene cover was found to be the best container,
and soilrite was the best medium at third fortnight onwards.
The containers showed no significant influence on petiole
length at second and fourth fortnight but at second fortnight
onwards medium soilrite recorded maximum petiole length. The
plants grown in polythene cover with media soilrite recorded
inaximum number of roots and length of roots at two months
after transplanting.
So it is evident that among the media,
soilrite was the best for ex vitro establishment of anthurium
plantlets but containers showed no uniform response with
various growth factors.
635 / AJI/ST