Unnikrishnan M P
Superovulatory Response, Embryo Collection and Transfer in Crossbred Cows - Mannuthy Department of Animal Reproduction, College of Veterinary and Animal Sciences 1996
Out of eleven crossbred cows superovulated with 2000 IU of
PMSG on day eleven and 25 mg PGF2 alpha 48 h later, nine
animals (81.8%) exhibited oestrum after an average interval of
39.44 ± 2.44 h. Average duration of oestrum was 40.00 ± 2.00
h with 66.7 per cent of them exhibiting intense heat signs and
33.3 per cent exhibiting only moderate heat signs. Average
number of corpora lutea and unovulated follicles in both the
ovaries put together was 6.67 ± 0.50 and 6.22 ± 0.57
respectively. Ovarian response was more in right ovary than in
left ovary, though not statistically significant.
Average fluid recovery on flushing was 79.5 per cent which
was comparatively low. The average embryo recovery and
percentage of embryo recovery were 3.38 ± 0.70 and 53.11 ±
10.18 respectively, which was comparatively lower. The reason
for poor recovery of embryo was attributed to poor fluid
recovery on flushing. The average number and percentage of
transferrable embryo recovered were 1.50 ± 0.53 and 33.34 ±
10.14. Reason for these lower rates were attributed to loss of
embryo quality, due to prolonged action of PMSG. Donors of
parity over three performed better on superovulation and
flushing, than those below three. Animals of age group six to
nine years produced more transferrable embryos than cows of age
group ten and above.
A conception rate of 30 per cent was achieved after
transfer of embryos to naturally synchronised recipients.
Heifers appeared to be better recipients than cows. Although
a high incidence of abortion was encountered, birth of an
embryo transfer male calf was also recorded in the study. All
the donor cows came into regular oestrous cycle within four
months of superovulation treatment.
636.082 / UNN/SU
Superovulatory Response, Embryo Collection and Transfer in Crossbred Cows - Mannuthy Department of Animal Reproduction, College of Veterinary and Animal Sciences 1996
Out of eleven crossbred cows superovulated with 2000 IU of
PMSG on day eleven and 25 mg PGF2 alpha 48 h later, nine
animals (81.8%) exhibited oestrum after an average interval of
39.44 ± 2.44 h. Average duration of oestrum was 40.00 ± 2.00
h with 66.7 per cent of them exhibiting intense heat signs and
33.3 per cent exhibiting only moderate heat signs. Average
number of corpora lutea and unovulated follicles in both the
ovaries put together was 6.67 ± 0.50 and 6.22 ± 0.57
respectively. Ovarian response was more in right ovary than in
left ovary, though not statistically significant.
Average fluid recovery on flushing was 79.5 per cent which
was comparatively low. The average embryo recovery and
percentage of embryo recovery were 3.38 ± 0.70 and 53.11 ±
10.18 respectively, which was comparatively lower. The reason
for poor recovery of embryo was attributed to poor fluid
recovery on flushing. The average number and percentage of
transferrable embryo recovered were 1.50 ± 0.53 and 33.34 ±
10.14. Reason for these lower rates were attributed to loss of
embryo quality, due to prolonged action of PMSG. Donors of
parity over three performed better on superovulation and
flushing, than those below three. Animals of age group six to
nine years produced more transferrable embryos than cows of age
group ten and above.
A conception rate of 30 per cent was achieved after
transfer of embryos to naturally synchronised recipients.
Heifers appeared to be better recipients than cows. Although
a high incidence of abortion was encountered, birth of an
embryo transfer male calf was also recorded in the study. All
the donor cows came into regular oestrous cycle within four
months of superovulation treatment.
636.082 / UNN/SU