Seema B J
Standardisation of in Vitro Techniques for Rapid Multiplication of Trichopus Zeylanicus Gaertn - Vellanikkara Department of Plantation crops and Spices, College of Horticulture 1997
Studies were conducted on standardisation of in vitro techniques for rapid
multiplication of Trichopus zeylanicus Gaertn at the Plant Tissue Culture Laboratory
of the Department of Plantation Crops and Spices, College of Horticulture,
Vellanikkara during 1995-1997.
Surface sterilization was standardised for different explants. Treatment
with 0.1 per cent mercuric choloride for 5 min was found to be the best for all the
explants. Explants collected during January to April showed lower contamination
and maximum survival.
Soaking seeds in water for one hour was found to reduce the number of
days for germination but lower germination percentage.
Young, purple shoots were observed to show maximum establishment
and growth. Establishment percentage and maximum number of buds was observed
to be highest in SH media supplemented with BA compared to 2iP and KIN.
Also exposure to light was favourable for better establishment of buds.
Proliferation rate was higher at higher concentration of BA but shoot
development was better at lower concentration of BA. Addition of adenine sulfate
increased the proliferation rate of buds and development of shoots but supplements
like yeast extract and casein hydrolysate were not effective in promoting shoot
growth.
Tender leaf and petiole explants were found to respond better than
mature explants and percentage of callus initiation and callus index was higher in
combinations of NAA and 2,4-D with BA. Direct organogenesis was observed in 1/2
MS supplemented with BA and NAA. Regeneration of healthy and longer shoots
were obtained in MS medium supplemented with KIN.
Somatic embryogenesis was observed in media containing BA and BA +
coconut water, and embryoid germination was obtained in MS medium.
Maximum rooting was obtained by culturing shoots in media containing
brassinolide for one week and thereafter transfer to IBA. Earlier rooting was
obtained in liquid medium.
Keeping in 1/2 MS with reduced sucrose and increased light intensity in
the culture room for two weeks before transfer to hardening unit resulted in better
survival of plantlets.
633.8 / SEE/ST
Standardisation of in Vitro Techniques for Rapid Multiplication of Trichopus Zeylanicus Gaertn - Vellanikkara Department of Plantation crops and Spices, College of Horticulture 1997
Studies were conducted on standardisation of in vitro techniques for rapid
multiplication of Trichopus zeylanicus Gaertn at the Plant Tissue Culture Laboratory
of the Department of Plantation Crops and Spices, College of Horticulture,
Vellanikkara during 1995-1997.
Surface sterilization was standardised for different explants. Treatment
with 0.1 per cent mercuric choloride for 5 min was found to be the best for all the
explants. Explants collected during January to April showed lower contamination
and maximum survival.
Soaking seeds in water for one hour was found to reduce the number of
days for germination but lower germination percentage.
Young, purple shoots were observed to show maximum establishment
and growth. Establishment percentage and maximum number of buds was observed
to be highest in SH media supplemented with BA compared to 2iP and KIN.
Also exposure to light was favourable for better establishment of buds.
Proliferation rate was higher at higher concentration of BA but shoot
development was better at lower concentration of BA. Addition of adenine sulfate
increased the proliferation rate of buds and development of shoots but supplements
like yeast extract and casein hydrolysate were not effective in promoting shoot
growth.
Tender leaf and petiole explants were found to respond better than
mature explants and percentage of callus initiation and callus index was higher in
combinations of NAA and 2,4-D with BA. Direct organogenesis was observed in 1/2
MS supplemented with BA and NAA. Regeneration of healthy and longer shoots
were obtained in MS medium supplemented with KIN.
Somatic embryogenesis was observed in media containing BA and BA +
coconut water, and embryoid germination was obtained in MS medium.
Maximum rooting was obtained by culturing shoots in media containing
brassinolide for one week and thereafter transfer to IBA. Earlier rooting was
obtained in liquid medium.
Keeping in 1/2 MS with reduced sucrose and increased light intensity in
the culture room for two weeks before transfer to hardening unit resulted in better
survival of plantlets.
633.8 / SEE/ST