Priya P M
Seroprevalence And Restriction Enzyme Analsis Of Egg Drop Syndrome Virus - Mannuthy Department of Microbiology, College of veterinary and Animal Sciences 2000
In the present study, seroprevalence of EDS-76 was
conducted in five districts of Kerala in duck and'
chicken flocks using HI and ELISA. Out of 322 chicken
and 281 duck sera samples screened,
an overall
incidence of 14.91 per cent and 26.69 per cent
respectively were recorded. The high proportion of
birds showing antibodies to EDS-76 reveals that the
infection is widespread in Kerala and may be the major
etiological
factor
associated
with
drop
in
egg
production in poultry.
Among the two serological tests namely, HI and
ELISA employed for the detection of EDS-76 viral
antibody, HI was found to be simple, sensitive and
reliable. It is concluded that HI test could be used
for the detection of EDS-76 infection in poultry
flocks.
Restriction
DNA fingerprinting
of
the
three
indigenous strains were carried out in conjunction with
the reference strain to check for any genetic variation
between the strains. Comparison of the DNA fingerprint
of all the four strains digested with restriction
endonucleases
BamHI,
EcoRI
and
HindIII
revealed
identical
banding pattern
thereby
conforming
the
genetic similarity of the strains.
636.089 6 / PRI/SE
Seroprevalence And Restriction Enzyme Analsis Of Egg Drop Syndrome Virus - Mannuthy Department of Microbiology, College of veterinary and Animal Sciences 2000
In the present study, seroprevalence of EDS-76 was
conducted in five districts of Kerala in duck and'
chicken flocks using HI and ELISA. Out of 322 chicken
and 281 duck sera samples screened,
an overall
incidence of 14.91 per cent and 26.69 per cent
respectively were recorded. The high proportion of
birds showing antibodies to EDS-76 reveals that the
infection is widespread in Kerala and may be the major
etiological
factor
associated
with
drop
in
egg
production in poultry.
Among the two serological tests namely, HI and
ELISA employed for the detection of EDS-76 viral
antibody, HI was found to be simple, sensitive and
reliable. It is concluded that HI test could be used
for the detection of EDS-76 infection in poultry
flocks.
Restriction
DNA fingerprinting
of
the
three
indigenous strains were carried out in conjunction with
the reference strain to check for any genetic variation
between the strains. Comparison of the DNA fingerprint
of all the four strains digested with restriction
endonucleases
BamHI,
EcoRI
and
HindIII
revealed
identical
banding pattern
thereby
conforming
the
genetic similarity of the strains.
636.089 6 / PRI/SE