Isolation and characterization of type III polyketide synthases from chethikoduveli (Plumbago rosea L) (Record no. 141392)
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| 000 -LEADER | |
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| fixed length control field | 03092nam a22002057a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | OSt |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20220418141310.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 220418b ||||| |||| 00| 0 eng d |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 660.6 |
| Item number | DHA/IS |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Dhanya Radhakrishnan |
| 245 ## - TITLE STATEMENT | |
| Title | Isolation and characterization of type III polyketide synthases from chethikoduveli (Plumbago rosea L) |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc. | Vellayani |
| Name of publisher, distributor, etc. | Department of Plant Biotechnology, College of Agriculture |
| Date of publication, distribution, etc. | 2014 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 112p |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | MSc(INT) |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | The study entitled “Isolation and characterization of type III polyketide synthases from chethikoduveli (Plumbago rosea Linn.)” was conducted at the Biotechnology and Bioinformatics Division, JNTBGRI, Palode, Thiruvananthapuram, during 2013-2014. The objective of the study was to isolate and sequence characterize type III polyketide synthase gene(s) involved in the biosynthesis of plumbagin in Plumbago rosea. This knowledge would eventually help in augmenting the production of plumbagin through the development of high yielding cell lines. Furthermore, chemical variation with respect to plumbagin content and genetic diversity using ISSR markers was also carried out in available accessions of Plumbago rosea. Nested degenerate primers specific to conserved regions of previously reported CHSs were used to amplify the cDNA synthesised from the reverse transcribed total RNA isolated from TBG-102. The amplicon was cloned and sequenced. The full length cDNA was elucidated using 5’ and 3’ RLM-RACE followed by cloning and sequencing. The full length cDNA was found to be 1,197 bp coding for 398 amino acids. The protein was calculated to have a molecular mass of 43.5 kDa and a theoretical pI of 5.89. BLASTX analysis of the full length cDNA showed 89 % identity with naringenin chalcone synthase from Hypericum androsemum, chalcone synthase from Hypericum sampsonii and aromatic polyketide synthase from Hypericum hookerianum. Simialr interpretation was drawn from the constructed phylogentic tree.In brief, it is concluded that the isolated full length cDNA belongs to typical CHS family and therefore, additional non-CHS class of PKSs would be involved in plumbagin synthesis. Genetic diversity analysis with ISSR markers revealed high degree of polymorphism as the primers generated 71 scorable bands, out of which 70 were polymorphic (98.6 % polymorphism). Thereby, the accessions were concluded to have high genetic diversity. 112 Chemical analysis in these accessions showed variation in plumbagin content from 0.2 % to 0.7 %. The observed variation in plumbagin content may be due to the prevailing high genetic diversity in these accessions and variation in edaphic factors. The plumbagin content in TBG-102 was estimated using HPLC and spectrophotometric analysis. Both methods produces comparable results. Therefore cold extraction method was found to be reproducible. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Plant Biotechnology |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | P Padmesh Pillai (Guide) |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | http://krishikosh.egranth.ac.in/handle/1/5810109534 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | |
| Koha item type | Theses |
| Withdrawn status | Lost status | Damaged status | Not for loan | Collection code | Permanent Location | Current Location | Shelving location | Date acquired | Full call number | Barcode | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Not For Loan | Reference Book | KAU Central Library, Thrissur | KAU Central Library, Thrissur | Theses | 2015-02-09 | 660.6 DHA/IS | 173447 | 2015-02-09 | 2015-02-09 | Theses |