Development of near isogenic lines of rice variety 'uma' for blast resistance genes through molecular marker assisted backcross breeding (Record no. 159992)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 06868nam a22001817a 4500 |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 630.28 |
| Item number | HAR/DE |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Harikrishnan, P J |
| 245 ## - TITLE STATEMENT | |
| Title | Development of near isogenic lines of rice variety 'uma' for blast resistance genes through molecular marker assisted backcross breeding |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc | Vellayani |
| Name of publisher, distributor, etc | Department of Plant Breeding and Genetics, College of Agriculture |
| Date of publication, distribution, etc | 2017 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 168p. |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | PhD |
| 520 3# - SUMMARY, ETC. | |
| Abstract | Blast disease, caused by Magnaporthe oryzae is one of the most devastating diseases in rice and is a great threat to food security worldwide. During kharif season, the disease is prevalent throughout the rice growing areas in India including the southern states of Kerala, Tamil Nadu, Karnataka and Andhra Pradesh. Approximately 100 major blast resistance genes have been reported in rice and hence exploitation of host plant resistance through gene pyramiding can be employed effectively for the management of blast. Developing Near Isogenic Lines (NILs) ie., lines carrying each of the major resistance genes in the background of susceptible recurrent parent is a major step in the pyramiding work to be carried out for developing multi race resistant varieties. So, the present study entitled “Development of Near Isogenic Lines of rice variety „Uma‟ for blast resistance genes through molecular marker assisted backcross breeding” was undertaken at the College of Agriculture, Vellayani Thiruvananthapuram, to develop Near Isogenic lines (NILs) of rice variety Uma for blast resistance genes (Pi1, Pi2 and Pikh) using identified donors through marker assisted back cross breeding. DNA markers closely linked to the blast resistance genes viz., RM527 (Pi2 gene), RM224 (Pi1 gene) and RM206 (Pikh gene) were used for validating marker polymorphism in the identified traditional donors of blast resistance genes viz. PTB21 (Thekkan) with Pi2, PTB7 (Parambuvattan) with Pi1 and Pikh and susceptible recipient parent Uma (MO16). This validation confirmed the absence of genes in the recipient parent, „Uma‟. These polymorphic gene specific markers were used for foreground selection in F1, BC1F1 and BC2F1 plants. Hybridisation was carried out between recipient parent Uma and the two donor parents viz., PTB21 and PTB7 to transfer genes for resistance. F1 plants with heterozygous loci for blast resistance genes specific markers were identified through foreground selection and backcrossed with „Uma‟ the recurrent parent to obtain the BC1F1 generation. The BC1F1 plants were screened with foreground markers and those plants containing the respective resistance genes were identified. Among the plants screened for a particular cross, 12 were found to contain Pi2 gene, 17 had Pi1 gene and 21 plants contained Pikh gene. χ2 test was done with the genotypic data of BC1F1 plants with single genes viz. Pi1, Pi2 and Pikh. Segregation ratio in BC1F1 population from all the crosses except Uma x PTB7 (Pi1) did not show significant deviation from the expected 1:1 ratio suggesting the presence of segregation distortion in the segregants of the cross Uma x PTB7 (Pi1). For the background selection, three parental lines were genotyped with 30 microsatellite loci and the ones polymorphic for Uma and the donors were identified. Twelve markers were found to produce discrete amplicons for distinguishing Uma and PTB21 while 17 markers could distinguish Uma and PTB7. Five of the polymorphic markers were used for background selection in BC1F1 plants with resistance genes of the three crosses. The BC1F1 plants with highest percentage of recurrent parent genome recovery in the three populations were identified. Highest recovery percentage of 70 could be obtained in backcross population of all the three crosses. Morphological data for seven quantitative and one qualitative character (kernel colour) was recorded for the BC1F1 plants with the resistant genes. Euclidean coefficient of dissimilarity was assessed in comparison with the recurrent parent Uma. The Euclidean distance values ranged from 5.9 (A-21) to 21.3 (A-9) in the cross Uma x PTB21 (Pi2), 4.88 (B-8) to 17.8 (B-23) in Uma x PTB7 (Pi1) and from 4.79 (C-34) to 13 (C-15) in case of Uma x PTB7 (Pikh). All the BC1F1 plants with resistance genes were used to raise the BC2F1 generation. The BC2F1 populations of the three crosses were subjected to foreground selection and plants with resistance genes were identified. Background selection was carried out in BC2F1 plants with resistance genes using 12 polymorphic markers in the cross Uma x PTB 21 (Pi2) and 17 markers in plants identified in the BC2F1 of Uma x PTB7 (Pi1 and Pikh). Percentage of recurrent parent genome recovery ranged from 70.83 to 95.83 for the cross Uma x PTB21 (Pi2), 70.59 to 97.06 for Uma x PTB7 (Pi1) and from 61.76 to 94.12 for Uma x PTB7 (Pikh). Euclidean coefficient of dissimilarity was assessed in comparison with the recurrent parent Uma, based on seven quantitative traits (plant height, number of tillers number of productive tillers, panicle length, grain L/B ratio, thousand grain weight and days to maturity) in the BC2F1 plants with resistance genes. The lowest values of Euclidean distance were recorded in the plants A-16-6 (2.24), B-18-2 (3.35) and C-17-3 (4.33) of the three backcross populations respectively. Based on the Euclidean distance and percentage recovery of the recurrent parent genome, five plants were identified from each of the three crosses as potential Near Isogenic Lines (NILs) for the three blast resistance genes Pi2, Pi1 and Pikh. The genotype A-5-1 with a recurrent parent genome recovery of 95.83% and Euclidean distance of 5.55 with Uma was identified as the most potential NIL of Uma for the gene Pi2. Similarly B-27-1 (Euclidean distance – 4.56, % genome recovery - 97.06) and C-17-3 (Euclidean distance - 4.33, % genome recovery - 94.12) were identified as potential NILs for Pi1 and Pikh genes respectively. Selfed seeds (BC2F2) were collected from the identified potential NILs. The present research could identify NILs of Uma for three blast resistance genes viz., Pi1, Pi2 and Pikh with more than 94% recurrent parent genome recovery with two backcrossing through marker assisted selection. This confirms the utility of marker assisted backcross breeding in recurrent parent genome recovery. The NILs can be used in intermating programmes in future to develop pyramided lines of Uma with all the three blast resistance genes (Pi1, Pi2 and Pikh) to ensure high yielding ability and durable broad spectrum resistance to the blast pathogen. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Agriculture |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Plant Breeding and Genetics |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Jayalekshmy, V G (Guide) |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | http://krishikosh.egranth.ac.in/handle/1/5810143535 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | |
| Item type | Theses |
| Not for loan | Collection code | Permanent location | Current location | Shelving location | Date acquired | Full call number | Barcode | Date last seen | Koha item type |
|---|---|---|---|---|---|---|---|---|---|
| Not For Loan | Reference Book | KAU Central Library, Thrissur | KAU Central Library, Thrissur | Theses | 2018-04-11 | 630.28 HAR/DE | 174200 | 2018-04-11 | Theses |