Evaluation of biochemical and anti-cancerous activities of mushrooms (Record no. 161922)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 04396nam a22001697a 4500 |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 632.3 |
| Item number | AGN/EV |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Agnes Jose |
| 245 ## - TITLE STATEMENT | |
| Title | Evaluation of biochemical and anti-cancerous activities of mushrooms |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc | Vellayani |
| Name of publisher, distributor, etc | Department of Plant Pathologoy, College of Agriculture |
| Date of publication, distribution, etc | 2018 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 137p. |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | MSc. |
| 520 3# - SUMMARY, ETC. | |
| Abstract | The present study on "evaluation of biochemical and anti-cancerous activities of mushrooms" was conducted during 2016-2018 with the objective to undertake the cultural, spawn production and cultivation trials of five mushrooms namely, Ganoderma lucidum (Curtis) P. Karst., Pleurotus florida (Mont.), Pleurotus djamor (Fr.) Boedjn, Hypsizygus ulmarius (Bull.:Fr.) Redhead and Calocybe gambosa (Fr.) Singh and to evaluate their biochemical and anti-cancerous activities. The cultures of these mushrooms were isolated from the mushroom beds maintained in the mushroom unit of Instructional Farm, Vellayani through tissue culture method and purified by hyphal tip method. Studies on mycelial growth of five mushrooms on potato dextrose agar revealed that G. lucidum had the maximum radial growth (9.00 cm) in shorter period of time (6.25 days). The nature of mycelial growth of G. lucidum and P. florida was flat and filamentous, while that of P. djamor, H. ulmarius and C. gambosa was thick and fluffy. The colour of mycelium of all the mushrooms was white to creamy white. Spawn production trials of five mushrooms on paddy grain indicated the minimum time for spawn run of 12.25 days for P. djamor followed by H. ulmarius and P. florida. Cultivation trials of P.djamor, P. florida, H. ulmarius and C. gambosa were undertaken on paddy straw substrate while that of G. lucidum was done on rubber wood sawdust. P. djamor recorded minimum time for spawn run (10.50 days), pinhead formation (14.50 days) and first harvest (16.50 days). The milky mushroom, C. gambosa recorded the maximum yield of 1037.25 g kg-1 from three harvests with 103.72 per cent biological efficiency (BE) followed by H. ulmarius with yield of 960 g kg-1 and BE of 96 %. Infestation of pests viz. phorid flies and staphylinid beetles as well as fungal contaminants such as Trichoderma sp., Aspergillus sp., and Coprinus spp. were found in all the mushrooms during sporocarp formation. Dried and powdered samples of mushrooms were used for nutritional analyses. G. lucidum recorded maximum protein (30.91 %) and fibre content (49.33 %), whereas C. gambosa recorded maximum carbohydrate, total amino acids, ash and fat content. The present study indicated the high nutritive value of milky mushroom C. gambosa Mineral components like sodium, potassium, phosphorus, calcium and magnesium were determined in the study. Among the five mushrooms C. gambosa recorded the maximum potassium while calcium and magnesium were the maximum in G. lucidum. The phosphorus content was not found to be significantly different among the five mushrooms. Analysis of medicinal components of mushrooms indicated that mushrooms were rich in beta-glucan, polyphenols, flavonoids and terpenoid. G. lucidum, the king of medicinal mushrooms recorded maximum beta-glucan (38.58 %) and polyphenols (23.80 mg GAE g-1) followed by C. gambosa. Flavonoid and terpenoid contents were recorded maximum in C. gambosa followed by G. lucidum. The pink oyster mushroom, P. djamor recorded the maximum beta- carotene content (355μg g-1). Preliminary trials on the anti-cancer activities of mushroom extracts were conducted by direct microscopic studies and MTT assay. The results revealed that percentage viability of cervical cancer cell lines decreased with increase in concentration of mushroom extracts. However G. lucidum extract exhibited maximum cytotoxic effect on cancer cell lines even at lower concentration (200 μg ml-1) followed by C. gambosa. The present study indicated that all the five mushrooms are rich source of protein, carbohydrate, fibre and minerals. P. florida, P. djamor, H. ulmarius and C. gambosa were nutritionally and medicinally superior. The commercial cultivation as well as clinical studies of these medicinally important mushrooms must be undertaken in large scale. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Plant Pathology |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Geetha, D (Guide) |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | http://krishikosh.egranth.ac.in/handle/1/5810145171 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | |
| Item type | Theses |
| Not for loan | Collection code | Permanent location | Current location | Shelving location | Date acquired | Full call number | Barcode | Date last seen | Koha item type |
|---|---|---|---|---|---|---|---|---|---|
| Not For Loan | Reference Book | KAU Central Library, Thrissur | KAU Central Library, Thrissur | Theses | 2018-09-27 | 632.3 AGN/EV | 174321 | 2018-09-27 | Theses |