Etiology of honey bee brood disease in Southern Kerala (Record no. 163735)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 04962nam a22001697a 4500 |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 632.6 |
| Item number | JYO/ET |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Jyothis P. Joseph |
| 245 ## - TITLE STATEMENT | |
| Title | Etiology of honey bee brood disease in Southern Kerala |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc | Vellayani |
| Name of publisher, distributor, etc | Department of Agricultural Entomology, College of Agriculture |
| Date of publication, distribution, etc | 2018 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 109p. |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | MSc |
| 520 3# - SUMMARY, ETC. | |
| Abstract | The study entitled “Etiology of honey bee brood disease in Southern Kerala” was conducted with the objective to characterize, identify and confirm the infectivity of the microbial isolate from diseased Indian honey bee brood and also to test the efficacy of botanicals against it. The study was carried out during the period 2016-2018 at AICRP on Honey bees and Pollinators, Department of Agricultural Entomology, College of Agriculture, Vellayani. Survey was conducted in the Indian bee apiaries of three Southern districts of Kerala viz. Thiruvananthapuram, Kollam and Pathanamthitta. From each district, five apiaries with a minimum of 10 colonies were selected purposively. The infected brood samples were subjected to laboratory study to understand the etiology of the disease. Evaluation of botanicals against the Indian bee brood disease using different botanicals was also conducted. Scattered presence of sealed and unsealed brood giving “pepper-box symptom”, change in colour of the larvae from pearly white to yellow and finally dark brown, perforated brood cappings with dead pupae and infected larvae transform into brown scales which were seen adhered to the bottom of the cell, were the typical symptoms observed in the infected bee colonies. Apart from these, the adult bees were found restless and more aggressive, reduced foraging activity, tendency for absconding and reduced honey yield were also observed. The monthly incidence of the disease varied among different locations with the peak infection period during the dearth season (May to August) in Thiruvananthapuram district, while in Kollam district it was observed in both dearth season and brood rearing season (Sept. to Dec.). Among the three districts, highest mean percentage infection was recorded from the apiaries of Kollam district (44.36) followed by Thiruvananthapuram district (4.45) and the least infection percentage from the apiaries of Pathanamthitta district (1.07). Three bacterial colonies were isolated (viz. J1, J2 and J3 isolates) in the nutrient agar (NA) medium from the infected brood samples taken during survey. The bacterial isolates, J1 and J3 which were proved pathogenic to the honey bee larvae were subjected to morphological, molecular and biochemical characterisation. The bacteria, identified as Bacillus pumilus and Achromobacter sp., has already been reported to be associated with Melissococcus plutonius, causal agent of European Foul Brood. Evaluation of botanicals for the management of Indian bee brood disease with crushed garlic (0.25 % and 0.5 %), crushed leaves of Centella asiatica (0.05 % and 0.1 %), crushed leaves of Ocimum sp. (0.05 % and 0.1 %), turmeric powder (0.2 %), CaSO4 (homeo medicine), along with oxytetracycline hydrochloride (antibiotic) (40 ppm) as check and a control provided with artificial feed, was assessed in terms of infected brood area, sealed brood area, unsealed brood area, pollen storage, honey storage and number of foragers per minute. After four weeks of treatment, bee colonies treated with crushed garlic 0.25 % and crushed leaves of Ocimum sp. 0.05 % recorded highest percentage reduction of the disease with a mean value of 68.52 and 67.57 respectively. Apart from these, a significant increase in sealed brood area (286.07 cm2 and 261.32 cm2), unsealed brood area (236.80 cm2 and 222.22 cm2), pollen storage (49.19 cm2 and 47.70 cm2), honey storage (84.74 cm2 and 77.13 cm2) and number of foragers (3.13 and 3 bees per minute) were also observed in the colonies treated with crushed garlic 0.25 % and crushed leaves of Ocimum sp. 0.05 % respectively, when compared to their respective control (34.62 cm2; 124.71 cm2; 14.88 cm2; 39.68 cm2and 1.13 bees per minute). Thus the present investigation revealed that the Indian bee apiaries of Kollam district recorded the highest incidence of brood disease in southern Kerala during the dearth and brood rearing season, with a mean percentage infection of 44.36 followed by Thiruvananthapuram district (4.45) and Pathanamthitta district (1.07) Two bacterial isolates which proved the pathogenicity were identified as Bacillus pumilus and Achromobacter sp. Crushed garlic 0.25 % and crushed leaves of Ocimum sp 0.05 % were found effective in the field evaluation of botanicals, against brood disease, with a mean percentage reduction of 68.52 and 67.57 respectively. - |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Agricultural Entomology |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Amritha, V S (Guide) |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | http://krishikosh.egranth.ac.in/handle/1/5810145827 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | |
| Item type | Theses |
| Not for loan | Collection code | Permanent location | Current location | Shelving location | Date acquired | Full call number | Barcode | Date last seen | Koha item type |
|---|---|---|---|---|---|---|---|---|---|
| Not For Loan | Reference Book | KAU Central Library, Thrissur | KAU Central Library, Thrissur | Theses | 2019-02-12 | 632.6 JYO/ET | 174382 | 2019-02-12 | Theses |