Somatic embryogenesis from immature inflorescence of coconut (Cocos nucifera L.) (Record no. 194372)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03046nam a22001697a 4500 |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 660.6 |
| Item number | RAD/SO PG |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Radhika R. |
| 245 ## - TITLE STATEMENT | |
| Title | Somatic embryogenesis from immature inflorescence of coconut (Cocos nucifera L.) |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc | Vellanikkara |
| Name of publisher, distributor, etc | Department of Plant Biotechnology, College of Horticulture |
| Date of publication, distribution, etc | 2019 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 74p |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | M.Sc. |
| 520 ## - SUMMARY, ETC. | |
| Abstract | Coconut palm (Cocos nucifera L.), the only species of genus Cocos, is a major crop plant which is propagated exclusively by seeds presently. Coconut is highly heterozygous, generally cross-pollinated and long lived. So the offspring differ greatly due to segregation, which can be assessed only after a very long juvenile phase. Breeding for crop improvement is thus a difficult and long-term process. So within a short period, in- vitro propagation, employing tissue culture techniques, offers a means of cloning improved planting material. So the objective of the study was to assess the in vitro response of immature inflorescence of coconut explants for induction and germination of somatic embryos by supplementing the medium with by-product of neera processing and neera. The media for culture initiation, subculturing and embryo germination were standardised in earlier experiments (Siny, 2006; Sujatha, unpublished data, 2011). In the present experiments, standardised sub-culturing medium with addition of different supplements were tested. Embryogenic calloids and somatic embryos were induced in standardized CM2 media (Y3 basal media containing a combination of 0.5 mgL-1 picloram, 1 mgL-1 NAA and 0.1 mgL-1 TDZ and 300-600 μM 2,4-D) from the immature inflorescence explant. Among the different stage and region of immature inflorescence tested in standardised CM2 medium, tip region of inflorescence (85.71%) with length 1-5 cm was found to be the best for calloid induction with low browning (4.76%). The adverse effect of exudation of polyphenols was reduced by inoculating youngest inflorescence with reduced thickness. Embryogenic calloids were sub- cultured into 10 media combinations. With four stages of explant (based on length), each stage with two types (tip or base) there were eighty combinations. The suitability of the by-product from neera processing as well as neera (CIS- Coconut Inflorescence Sap) as supplements for CM2 medium to culture the immature inflorescence of coconut was assessed and found that CM2 medium with 0.3 per cent activated charcoal, 4 per cent sucrose and 2 per cent supplement and also CM2 medium with 0.3 per cent activated charcoal, 4 per cent sucrose and 10 mlL-1 CIS was the best medium for obtaining cultures with calloid proliferation (92.75 % & 90.17 %) with minimal phenolic interference (11.58 % & 22.7%). Calloid proliferation was successfully achieved from all the media combinations. Chemical properties of neera supplement used in the culture medium |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Plant Biotechnology |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Sujatha R. |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | https://krishikosh.egranth.ac.in/handle/1/5810156864 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | |
| Item type | Theses |
| Not for loan | Collection code | Permanent location | Current location | Shelving location | Date acquired | Full call number | Barcode | Date last seen | Koha item type |
|---|---|---|---|---|---|---|---|---|---|
| Not For Loan | Reference Book | KAU Central Library, Thrissur | KAU Central Library, Thrissur | Theses | 2020-05-08 | 660.6 RAD/SO PG | 174835 | 2020-05-08 | Theses |