Varietal screening of black pepper to cucumber mosaic virus and Piper yellow mottle virus and their sero molecular detection (Record no. 195233)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 08739nam a22001817a 4500 |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 632.3 |
| Item number | ARY/VA PG |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Arya M |
| 245 ## - TITLE STATEMENT | |
| Title | Varietal screening of black pepper to cucumber mosaic virus and Piper yellow mottle virus and their sero molecular detection |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc | Vellayani |
| Name of publisher, distributor, etc | Department of Plant Pathology, College of Agriculture |
| Date of publication, distribution, etc | 2020 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 124p. |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | MSc |
| 520 3# - SUMMARY, ETC. | |
| Abstract | ABSTRACT Varietal screening of black pepper to Cucumber mosaic virus and Piper yellow mottle virus and their sero-molecular detection The study entitled “Varietal screening of black pepper to Cucumber mosaic virus and Piper yellow mottle virus and their sero-molecular detection’ was conducted at Department of Plant Pathology, College of Agriculture, Vellayani during 2018-2020 with the objectives to study the occurrence and sero-molecular detection of Cucumber mosaic virus (CMV) and Piper yellow mottle virus (PYMoV) infecting black pepper and to identify the varietal response to infection by the viruses. As a part of the study, survey was conducted in four panchayats of Idukki (Erattiyar, Kattappana, Pampadumpara and Karunapuram) and Wayanad (Ambalavayal, Meenangadi, Poothadi and Panamaram) districts of Kerala. Plant samples showing the symptoms of viral infections were collected and severity of viral infections were assessed from farmer’s field at the survey locations. Highest disease incidence (DI) was observed in Poothadi (45.50 %) of Wayanad and lowest in Karunapuram (21.62 %) of Idukki district. Highest vulnerability index (V.I.) was recorded in Panamaram (61) of Wayanad and lowest in Pampadumpara (32) of Idukki district. Mealy bugs were found associated with the virus infected plants in survey locations. Different symptoms of viral infections observed in the field were general chlorosis, initial chlorotic specks progressing to severe mottling and curling, vein banding, vein clearing, leaf distortion and reduction in leaf size. The virus inoculum was maintained in insect proof greenhouse condition. In the field, high viral disease incidence was observed in Karimunda plants while Thekkan pepper grafted on Piper colubrinum did not express any symptom. Varietal screening with 15 varieties (KAU, IISR and local) was undertaken by wedge grafting the infected scions showing the symptoms of both viruses (CMV and PYMoV) on to healthy black pepper plants. Among the varieties screened, Karimunda was identified as the most susceptible variety with highest V.I. (48). Serological detection of CMV and PYMoV were confirmed using polyclonal antisera of Cucumber mosaic virus (CMV) and two monoclonal antibodies of Banana streak virus (BSV 1 and BSV 2; as PYMoV belongs to the same group of BSV) through DAS (Double Antibody Sandwich)-ELISA. Infection due to either of the two viruses and mixed infections by both viruses were detected in the leaf samples. Leaf curling was found associated with infection by PYMoV while leaf reduction and distortion in leaf size were found associated with CMV infection. The results were confirmed using polyclonal antibodies of CMV and Sugarcane bacilliform virus (SCBV; as PYMoV belongs to the same group of SCBV) through DIBA (Dot Immuno Binding Assay) in infected leaf samples. The quality and quantity of isolated DNA by CTAB method was also assessed. PCR based molecular detection of PYMoV using ORF III specific primers yielded amplicons of approximately 400 bp. The amplified PCR products were subjected to sequencing and blast analysis. All the isolated sequences were found to be related to PYMoV. Phylogenetic studies revealed that isolates from Idukki and Wayanad grouped in separate clades. The present study on viruses infecting black pepper indicated that infection of CMV and PYMoV either in pure form or as mixed infections was prevalent in cultivated areas of Idukki and Wayanad districts of Kerala. Serological techniques like DASELISA and DIBA could be used for detection of these viruses along with molecular detection using coat protein specific primers. Varietal screening undertaken revealed that Karimunda was the most susceptible variety. The isolates of PYMoV from different locations of Idukki and Wayanad showed similarity in ORF III to other reported PYMoV sequences. സംഗ്രഹം കുരുമുളകിലെ വൈറസ് രരോരങ്ങൾലെതിലര ഇനങ്ങളുലെ ഗ്രതികരണൈും കുരുമുളകിലെ വൈറസ് രഹതുെളുലെ സീരറോ രമോളിെുെോർ രഠനൈും എന്ന ൈിഷയത്തിൽ രഠനം ലൈള്ളോയണി കോർഷിക രകോളജിലെ സസൃരരോര ൈിഭോരത്തിൽ 2018-20 കോെയളൈിൽ നെത്തുകയുണ്ടോയി. രഠനത്തിൻ്്ലറ ഭോരമോയി കുരുമുളക് കൃഷി ൈൃരകമോയി നെത്തുന്ന ഇെുെി, ൈയനോെ് ജില്ലകളിലെ 4 രഞ്ചോയത്തുകളിൽ സർരൈ നെത്തുകയുണ്ടോയി.അതിനോയി ഇെുെിയിലെ ഇരട്ടിയോർ, കട്ടപ്പന, രോമ്പോെുംരോറ, കരുണോരുരം എന്നീ രഞ്ചോയത്തുകളും ൈയനോെ് ജില്ലയിൽ നിന്നും അമ്പെൈയൽ, മീനങ്ങോെി, രൂതോെി, രനമരം എന്നീ രഞ്ചോയത്തുകളും സന്ദർശിച്ചു.വൈറസ് രരോര െക്ഷണം ഗ്രകെിപ്പിച്ച കുരുമുളക് ൈള്ളികൾ രശഖരിെുകയും അൈയുലെ രരോര ൈൃരനൈും രരോര തീഗ്ൈതയും ൈിെയിരുത്തുകയുണ്ടോയി.ഏറ്റൈും കൂെുതൽ രരോരത്തിന്ലറ ൈൃരനം റിരപ്പോർട്ട് ലെയ്തത് ൈയനോട്ടിലെ രൂതോെി രഞ്ചോയത്തിെും (45.5 %) ഏറ്റൈും കുറൈ് ഇെുെിയിലെ കരുണോരുരം രഞ്ചോയത്തിെും ആണ്.രരോര ൈൃരനത്തിന് രഹതുെളോയ മുഞ്ഞ, മീെിമൂട്ട തുെങ്ങിയൈയുലെ സോന്നിധ്യൈും ൈള്ളികളിൽ നിന്നും രശഖരിച്ചു. ലകോെികളിൽ കണ്ട രെതരം രരോര െക്ഷണങ്ങളും രഠിെുയുണ്ടോയി.മഞ്ഞളിപ്പ്, കുരുെിപ്പ് തുെങ്ങിയൈ ആണ് ഗ്രധ്ോന രരോര െക്ഷണങ്ങൾ. ൈിൈിധ്ഇനങ്ങളുലെ രരോര ഗ്രതിരരോധ് രശഷി അളെുന്നതിനോയി രരോരമുള്ള ൈള്ളി രരോര രഹിതമോയ ൈള്ളികളിൽ ഗ്രോഫ്റ്റ്റ് ലെയ്തു. കരിമുണ്ട എന്ന ലകോെിയിനത്തിനോണ് ഗ്രതിരരോധ് രശഷി ഏറ്റൈും കുറൈ് എന്ന് കലണ്ടത്തി. രുതിയ ഇെകളിൽ രരോരെക്ഷണങ്ങൾ ഗ്രകെമോെുന്ന ദിൈസൈും അൈയുലെ തീഗ്ൈതയും രഠിച്ചു.രരോരം നിജലപ്പെുത്തുന്നതിനോയി സീരറോ രമോളിെുെോർ രീതികളോയ എെിസ, ഡിബ, രി. സി. ആർ എന്നീ ലെസ്റ്റ്കൾെ് ൈിരധ്യമോെി ലകോണ്ട് വൈറസ് സോന്നിധ്യം സ്ഥിരികരിച്ചു. രരോരകോരിയോയ ലരപ്പർ ലയരല്ലോ രമോട്ടൽ വൈറസിന്ലറ ഒ.ആർ.എഫ്റ് 3 ജനിതക മോഗ്തകളും ഇൈയ്െ് മറ്റ് വൈറസുകളുമോയി തോരതമൃ രഠനൈും (വഫ്റരെോജനി ഗ്െീ) നെത്തി. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Viruns on black pepper |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | sero molecular detection |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Radhika N S (Guide) |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | https://krishikosh.egranth.ac.in/handle/1/5810156879 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | |
| Item type | Theses |
| Not for loan | Collection code | Permanent location | Current location | Shelving location | Date acquired | Full call number | Barcode | Date last seen | Koha item type |
|---|---|---|---|---|---|---|---|---|---|
| Not For Loan | Reference Book | KAU Central Library, Thrissur | KAU Central Library, Thrissur | Theses | 2020-11-25 | 632.3 ARY/VA PG | 174887 | 2020-11-25 | Theses |