Somatic Embryogenesis in Cocoa (Theobroma cacao L.) (Record no. 25378)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03294nam a2200193Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | OSt |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20220205175907.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 140128s9999 xx 000 0 und d |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 630.28 |
| Item number | JOL/SO |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Jolly Antony |
| 245 ## - TITLE STATEMENT | |
| Title | Somatic Embryogenesis in Cocoa (Theobroma cacao L.) |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc. | Vellanikkara |
| Name of publisher, distributor, etc. | Department of Agricultural Botany, College of Horticulture |
| Date of publication, distribution, etc. | 1993 |
| 502 ## - DISSERTATION NOTE | |
| Degree type | MSc |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Investigation on somatic embryogenesis in cocoa (Theobroma cacao L.) were carried out at the college of Horticulture, Kerala Agricultural University, Vellanikkara during the period 1989-91, with the objective of studying the developmental potential of somatic embryos and its differentiation into plantlet by means of in vitro techniques. Stem and leaf segments, cotyledons and enbryonic axes of embryos collected from four typical genotypes of cocoa namely criollo, Amelonado, Amazon-forastero and Trinitario were used as explant. Cotyledons and embryonic axes of immature embryos (100 days pot anthesis) when incubated on MS basal semisolid medium supplemented with NAA 2 mg/1, thiamine 1 mg/1, casein hydrolysate 0.2 per cent (W/V) and coconut water 15 per cent (v/v) under dark for seven weeks resulted in high frequency and intensity of embryogenesis. Stem segements remined recalcitrant without embryoid regeneration, while leaf segments had a little potential. Auxins conditioned the culture for embryogenic competence while cytokinins had an inhibitory effect. The effect of NAA 2ppm was not replaceable by other auxins such as IAA, 2, 4-D. 2,4-D was a poor quantitative and qualitative stimulant of embryogenesis. Studies on auxincytokinin interaction revealed the counteracting effect of cytokinin on auxin. Fully mature embryoids germinated in hormone-free liquid medium consisting of half the salt concentration of MS and 5 per cent sucrose when incubated at 3000 lux (16 hours) for two weeks. De-cotyledonisation of embryoids and its rinsing with sterile distilled water and dessication, each for three minutes, enhanced the differentiation into plantlet. Shoot growth was stimulated by exogenous supply of NAA, GA3 and coconut water. In vitro rooting was promoted by reducing the salt concentration of MS medium to half strength and supplementing with IBA and activated charcoal. Germination and regeneration of embryoid into plantlet was dependent on its size. Sizes ranging from 0-4 mm were sub-optimal for germination and differentiation. Larger embroids (4-6 mm) had greater potential for differentiation. Quantitative and qualitative differnces were expressed by cocoa genotypes. Amelonado was found to be superior to Criollo and Amazon types for the induction of embryogenesis from cotyledons. Trinitario was the least efficient. Embryogenic potential of Amazon embryonic axes was superior to Criollo and Amelonado types. Trinitario embryonic axes remained recalcitrant. Plantlets were derived from embryoids within a time span of 13 weeks in Amelonado, Criollo and Amazon types. |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Achamma Oommen (Guide) |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | https://krishikosh.egranth.ac.in/handle/1/5810102325 |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | https://krishikosh.egranth.ac.in/displaybitstream?handle=1/5810102325&fileid=a13bac7f-f43d-4805-9adf-e9c1333790b2 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | |
| Koha item type | Theses |
| Withdrawn status | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Permanent Location | Current Location | Shelving location | Date acquired | Full call number | Barcode | Date last seen | Price effective from | Koha item type |
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| KAU Central Library, Thrissur | KAU Central Library, Thrissur | Theses | 2014-03-18 | 630.28 JOL/SO | 170423 | 2014-03-18 | 2014-03-18 | Theses |