Secretory immunoglobulins of the Duck (Anas platyrrhynchos domesticus) (Record no. 25748)

000 -LEADER
fixed length control field 02618nam a2200181Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field OSt
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20220822104741.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 140128s9999 xx 000 0 und d
082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER
Classification number 636.089 6
Item number HAR/SE
100 ## - MAIN ENTRY--PERSONAL NAME
Personal name Hari Kumar A V
245 ## - TITLE STATEMENT
Title Secretory immunoglobulins of the Duck (Anas platyrrhynchos domesticus)
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Place of publication, distribution, etc. Mannuthy
Name of publisher, distributor, etc. Department of Microbiology, College of Veterinary and Animal Sciences
Date of publication, distribution, etc. 1996
502 ## - DISSERTATION NOTE
Degree type MVSc
520 3# - SUMMARY, ETC.
Summary, etc. The profile and functional properties of the immunoglobulins of serum, bile, mucos of trachea and intestine of ducks were studied.

The immunoglobulins were separated by salting out using ammonium sulphate. The various fractions of immunoglobulines were further resolved by Sephadex G-200 gel filteration which gave two peaks for serum and tracheal immunoglobulins and a single peak each for bile and intestinal immunoglobulins. The purity of these fractions were checked by immunodiffusion and immunoelectrophoresis. The different fractions obtained were quantified by single radial immunodiffusion. The level of fraction 1 in the bile ranged between 1718 µg/ml and 1959 µg/ml and that of serum, 1718.06 µg/ml to 2442 µg/ml, in the S.typhimurium treated groups. The level of fraction 1 in the NDV treated groups ranged from 1115 µg/ml to 1597.35 µg/ml in bile, and 1597.35 µg/ml to 1959.34 µg/ml in serum. The level of fraction 2 in serum ranged from 1797 µg/ml to 2591 µg/ml in S.typhimurium treated group and 1400 µg/ml to 1797 µg/ml in the NDV treated group. Fraction 2 was not detectable in bile.

The antibody response of ducks to a bacterial and viral antigen (anaculture of S.typhimurium and R2B strain of New Castle Disease virus respectively) was assessed.

On conducting standard tube agglutination test, the serum, bile and oviduct washings revealed antibody tires against S.typhimurium in inoculated birds ranging between 1:20 and 1:160 in the case of serum; 1:10 and 1:80 in the case of bile and tire less than 1:10 for oviduct washings. No antibody titre could be detected for tracheal and intestinal washings and testicular extracts.

The HI titre ranging from 1:32 to 1:128 could be observed for serum, 1:32 to 1:64 for bile and a titre of 1:16 was observed for oviduct washings of ducks parenterally administered with NDV. The tire was relatively low for serum when NDV was administered intranasaly. Intestinal and tracheal washings and testicular extract failed to reveal any HI antibodies.
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name James P C (Guide)
856 ## - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier http://krishikosh.egranth.ac.in/handle/1/5810134321
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme
Koha item type Theses
Holdings
Withdrawn status Lost status Source of classification or shelving scheme Damaged status Not for loan Permanent Location Current Location Shelving location Date acquired Full call number Barcode Date last seen Price effective from Koha item type
          KAU Central Library, Thrissur KAU Central Library, Thrissur Theses 2014-03-18 636.089 6 HAR/SE 170793 2014-03-18 2014-03-18 Theses
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