Screening And Biochemical Characterization Of Tomato Genotypes For Resistance To Bacterial Wilt (Record no. 26495)

000 -LEADER
fixed length control field 03167nam a2200181Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field OSt
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20231115111125.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 140128s9999 xx 000 0 und d
082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER
Classification number 635.6
Item number SUB/SC
100 ## - MAIN ENTRY--PERSONAL NAME
Personal name Subash Chandra Bose S
245 ## - TITLE STATEMENT
Title Screening And Biochemical Characterization Of Tomato Genotypes For Resistance To Bacterial Wilt
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Place of publication, distribution, etc. Vellanikkara
Name of publisher, distributor, etc. Department of Olericulture, College of Horticulture
Date of publication, distribution, etc. 1999
502 ## - DISSERTATION NOTE
Degree type MSc
520 3# - SUMMARY, ETC.
Summary, etc. Investigation on 'Screening and biochemical characterization of tomato
genotypes for resistance to bacterial' wilt' was carried out in the Department of
Olericulture, and Biochemistry Laboratory, College of Horticulture, Vellanikkara
1
during 1997-99. The objectives of this study were to identify tomato genotypes
resistant to bacterial wilt and to find the possibility for biochemical cataloguing of
bacterial wilt resistant tomato genotypes.
Evaluation for bacterial wilt resistance revealed that Sakthi, Mukthi and
LE-474 were consistently resistant to bacterial wilt. Four additional sources of
bacterial wilt resistance were identified, viz. LE-214, LE-415, LE-470 and LE-421.
Based on the percentage wilting the twenty four genotypes included in this study
were classified into four groups, viz. Resistant, Moderately Resistant, Susceptible
and Highly susceptible. -.:
All the bacterial wilt resistant and moderately resistant genotypes had a
higher content of total phenols in roots and leaves at 45th and 60th day of plant
growth;
O.D. phenol content in roots of resistant varieties were higher than
susceptible varieties. In leaf, the O.D. phenol content was high in moderately
resistant genotypes at 45th and 60th day.
All the genotypes were studied for isozyme variation with respect to two
enzymes, viz. polyphenol oxidase and peroxidase. 10 general the roots had more
number of bands compared to leaves at both the stages.
'.. The root samples at 45th day showed two polyphenol oxidase bands, viz.
PPO-I (~=0.074) and PPO-12 (Rm=0,414) which were specific to resistant
varieties alone. This combination along with high total phenols may be considered
as a biochemical marker for resistance to bacterial wilt in tomato. In 60 days old


-

roots of resistant and moderately re istant genotypes the protein band PPO-9
(Rm=0.851) was predominantly present
Regarding peroxidase, 45 . days old leaf samples had a combination of
protein bands, viz. PRX-7 (Rm=0.361) and PRX-8 (Rm=O.382) in resistant and
moderately resistant varieties. But at 60 days the roots and leaves did not show any
specific band for resistance.
In general at 45 days both leaf and root samples had more number of
bands for polyphenol oxidase and peroxidase in resistant varieties compared to
susceptible varieties. The study revealed that it was possible to arrive at a
combination of specific isozyme bands at a particular growth stage, which can be
used as a marker for bacterial wilt resistant tomato genotypes.

700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Rajan S (Guide)
856 ## - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier http://krishikosh.egranth.ac.in/handle/1/5810103665
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme
Koha item type Theses
Holdings
Withdrawn status Lost status Source of classification or shelving scheme Damaged status Not for loan Permanent Location Current Location Shelving location Date acquired Full call number Barcode Date last seen Price effective from Koha item type
          KAU Central Library, Thrissur KAU Central Library, Thrissur Theses 2014-03-18 635.6 SUB/SC 171552 2014-03-18 2014-03-18 Theses
Kerala Agricultural University Central Library
Thrissur-(Dt.), Kerala Pin:- 680656, India
Ph : (+91)(487) 2372219
E-mail: librarian@kau.in
Website: http://library.kau.in/