Standardisation of in vitro techniques for the rapid clonal propagation of bael (Aegle marmelos (L) corr) (Record no. 26863)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03459nam a2200181Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | OSt |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20220830161453.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 140128s9999 xx 000 0 und d |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 633.8 |
| Item number | HAZ/ST |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Hazeena M S |
| 245 ## - TITLE STATEMENT | |
| Title | Standardisation of in vitro techniques for the rapid clonal propagation of bael (Aegle marmelos (L) corr) |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc. | Vellayani |
| Name of publisher, distributor, etc. | Department of Plantation Crops and Spices, College of Agriculture |
| Date of publication, distribution, etc. | 2001 |
| 502 ## - DISSERTATION NOTE | |
| Degree type | MSc |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Studies were conducted for evolving in vitro techniques for the rapid clonal propagation of bael [ Aegle marmelos CL.) Corr.] during 1999 - 2001 at Plant Molecular Biology and Biotechnology Centre, College of Agriculture, Vellayani. Attempts were made to standardise in vitro propagation techniques of bael via enhanced release of axillary buds using shoot nodal segments and cotyledons as explants. Entire cotyledons were used as explants for direct organogenesis while cotyledons excluding embryoaxes were used for indirect organogenesis. Among the explants used for enhanced release of axillary buds, cotyledons responded better than nodal segments. Cent per cent survival could be obtained in cultures with cotyledons while a maximum of 50.00 per cent was obtained in cultures with nodal segments. Maximum shoot proliferation from nodal segments was obtained on full strength MS basal medium supplemented with BA 2.50 mgl ", IAA 1.00 mgl ", sucrose 30.00 gr' and agar 8.00 gl'. The best treatment identified for shoot proliferation from cotyledons was full strength MS basal medium supplemented with BA 0.50 gl ", GA3 3.00 mgl', adenine sulphate 20.00 mgl", sucrose 50.00 gl' and agar 5.00 gr'. Maximum initiation of direct organogenesis from cotyledons ( 83.33 per cent ) occurred in two treatments namely, on full strength MS basal ~--------------~~~.---------------------- medium supplemented with BA 0.10 mgl ", "sucrose 30.00 gr', and agar 8.00 gr' and on the same basal medium with BA 0.20 mgl ", sucrose 30.00 gl " and agar 8.00 gr'. The best treatment identified for highest number of shoots per culture was full strength MS medium supplemented with BA 0.40 mgl ", sucrose 30.00 gr' and agar 8.00 gri. Maximum proliferation of shoots on further subculturing was obtained on full strength MS medium supplemented with BA 0.20 mgl", IAA 2.00 mgl ", sucrose 30.00 gr' and agar 8.00 gr'. Direct organogenesis from in vitro root could be best obtained on full strength MS medium supplemented with BA 0.50 rngl", sucrose 30.00 gr' and agar 8.00 gr'. The best treatment identified for callus initiation was full strength MS medium supplemented with BA 0.50 mgl', 2,4-D 0.50 rngl ", sucrose 30.00 gr' and agar 8.00 gr' which recorded the highest callus index (350.00). Ideal treatment for the maximum proliferation from callus via indirect somatic organogenesis was found to be full strength MS medium with BA 2.00 rngl ", IAA 0.50 mgl", sucrose 30.00 gr' and agar 8.00 gr'. In vitro rooting occurred at its best on full strength MS medium supplemented with IBA 2.50 mgl ", sucrose 20.00 mgl' and agar 8.00 gr'. Pre-treatment with IBA 1000.00 ppm for 20 seconds proved to be the best for ex vitro rooting. Sand was the ideal potting media for ex vitro establishment. |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Sulekha G R (Guide) |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | http://krishikosh.egranth.ac.in/handle/1/5810105686 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | |
| Koha item type | Theses |
| Withdrawn status | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Permanent Location | Current Location | Shelving location | Date acquired | Full call number | Barcode | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| KAU Central Library, Thrissur | KAU Central Library, Thrissur | Theses | 2014-03-18 | 633.8 HAZ/ST | 171922 | 2014-03-18 | 2014-03-18 | Theses |