Exploitation Of Induced Variability For Crop Improvement In Ginger (Zingiber officinale Rosc.) (Record no. 27034)
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| fixed length control field | 06267nam a2200181Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | OSt |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20220905104113.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 140128s9999 xx 000 0 und d |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 633.8 |
| Item number | SHA/EX |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Shankar G |
| 245 ## - TITLE STATEMENT | |
| Title | Exploitation Of Induced Variability For Crop Improvement In Ginger (Zingiber officinale Rosc.) |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc. | Vellanikkara |
| Name of publisher, distributor, etc. | Department of Plantation Crops and Spices, College of Horticulture |
| Date of publication, distribution, etc. | 2003 |
| 502 ## - DISSERTATION NOTE | |
| Degree type | MSc |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Investigations on "Exploitation of induced variability for crop improvement in ginger (Zingiber officinale Rosc.)" were carried out at Department of Plantation Crops and Spices and Centre for Plant Biotechnology and Molecular Biology, College of Horticulture, V ellanikkara during 2001- 2003. Evaluation of seven induced variants along with three check varieties indicated the superiority of the autotetraploids V 2 (Z - 0 - 86) and V I (Z - 0 - 78) in quality attributes. V 2, an autotetraploid from Rio-de-Janeiro was notable for lowest fibre content, highest oil and oleoresin content and increased driage and high cuticle thickness than its diploid counterpart. V!, an autotetraploid from Himachal Pradesh exhibited highest driage, high oleoresin content and highest cuticle thickness. V 2 was more vigorous in growth exhibiting maximum yield contributing characters such as number of tillers per plant, number of leaves per plant, leaf area and also leaf area index and root length. V I showed maximum plant height and pseudo stem height which are correlated with yield. The fresh and dry yield of rhizomes of V I and V 2 were found to be on par with V 8 the highest fresh rhizome yielder and V 10 the highest dry rhizome yielder. V 3, a variant from Himachal Pradesh also produced yield on par with the highest yielder. The yielding capacity of the autotetraploids especially V 2 was found to be superseded by the incidence of diseases like soft rot and bacterial wilt. V I was least susceptible to leaf spot. V 10 (Himachal Pradesh) showing stable performance over the years had low incidence of soft rot and bacterial wilt. The high pollen fertility arid viability of the autotetraploids V I and V 2 and the highest pollen size and tube length observed in V 2 make them ideal as pollen parents in hybridization programmes. None of the various in vivo pollination techniques such as stylar, stigmatic, intra ovarian was effective for inducing fruit set or seed set in ginger. In vitro placental pollination with pollen grains suspended in ME3 medium was found to the best with maximum ovule swelling and maximum percentage of cultures with developed ovules (90 %). Pollen pistil interaction studies substantiated the lack of seed set under natural conditions due to failure of pollen germination owing to incompatibility barrieres operating in stigma, style and ovary. Elimination of these barriers in in vitro placental pollination enabled pollen germination and enough tube length to bring fertilization. The experiments on culture establishment showed that MS semi solid medium at half strength supplemented with NAA (0.5 mg rl) + BAP (2.5 mg rl) along with 3 % sucrose + CH (200 mg rl) or CW (15 %) v/v supported maximum development of ovules. Addition of double the quantity of the vitamin stock of the MS medium to the culture establishment medium and incubation under dark conditions also favoured more ovule development. Semi solid medium was advantageous in obtaining maximum seed set and size at maturity. Controlled selfing and crossing among the autotetraploids and diploid cultivars using in vitro placental pollination were successful and influenced ovule development. Mid season of flowering favoured maximum culture establishment and ovule development compared to early and late season in both selfing and crossing. Crossing enabled production of maximum number of developed ovules compared to selfing. Ovules/seeds developed in vitro were creamy white during the initial stage of development which changed to purple red colour with in a period of 30 - 35 DAP and to black with in a period of 55 - 80 DAP. The small arillate seeds had two seed coats, outer one being thick and inner one being thin. The seed coat encloses a cavity, which is typical of monocots. In the cavity, endosperm with embedded embryo is seen. Tetrazolium staining of well developed seeds revealed that the embryos were viable staining red which was more intense at 40 DAP than at 80 DAP seeds. Seed germination studies indicated that subjecting the seeds to embryo rescue (embryo culture) and in vivo seed treatments failed to induce germination. Under in vitro conditions, the seed of 80 DAP obtained from the cross diploid (Rio-de-Janeiro) X tetraploid (Z - 0 - 78) germinated after seed priming treatment (50 ppm KN03 for 8 h) and also the cross of tetraploid (Z - 0 - 86) X diploid (Maran) after seed imbibition in water for one day when cultured in half MS + 2, 4 - D (0.5) mg r', BAP (6.0) mg r' and GA3 (5.0) mg r' along with 3 per cent sucrose, showing good root development. Seed of the cross between diploid (Rio-de-Janeiro) X diploid (Maran) and diploid (Rio-de- Janeiro) X tetraploid (Z - 0 - 86) germinated showing radicle emergence after culturing in solid medium of half MS + 2, 4 - D (0.25) mg r' and BAP (9.0) mg r' along with 3 per cent sucrose. Somatic embryoids were developed from seeds cultured in media combinations of half MS + 2, 4 - D (0.5) mg r', BAP (6.0) mg r' and GA3 (5.0) mg r' along with 3 per cent sucrose and also in half MS + 2, 4 - D (0.1) mg r', BAP (8.0) mg r' along with 3 per cent sucrose in crosses between diploid and tetraploid. Plant regeneration from the somatic embryoids was not observed even after subjecting to media with different concentrations of various growth regulators. Callus growth was also observed after 25 days of seed germination in the seed culture medium containing half MS + 2, 4 - D (0.5 mg r'), BAP (6.0 mg r') and GA3 (5.0 mg r') along with 3 per cent sUcrose. |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Alice Kurian (Guide) |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | http://krishikosh.egranth.ac.in/handle/1/5810097651 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | |
| Koha item type | Theses |
| Withdrawn status | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Permanent Location | Current Location | Shelving location | Date acquired | Full call number | Barcode | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| KAU Central Library, Thrissur | KAU Central Library, Thrissur | Theses | 2014-03-18 | 633.8 SHA/EX | 172093 | 2014-03-18 | 2014-03-18 | Theses |