Molecular genetics diversity in dwarf cattle of Kerala (Record no. 27125)
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000 -LEADER | |
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fixed length control field | 04170nam a2200181Ia 4500 |
003 - CONTROL NUMBER IDENTIFIER | |
control field | OSt |
005 - DATE AND TIME OF LATEST TRANSACTION | |
control field | 20220303155943.0 |
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
fixed length control field | 140128s9999 xx 000 0 und d |
082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
Classification number | 636.082 |
Item number | SUP/MO |
100 ## - MAIN ENTRY--PERSONAL NAME | |
Personal name | Suprabha P |
245 ## - TITLE STATEMENT | |
Title | Molecular genetics diversity in dwarf cattle of Kerala |
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
Place of publication, distribution, etc. | Mannuthy |
Name of publisher, distributor, etc. | Department of Animal Breeding and Genetics, College of Veterinary and Animal Sciences |
Date of publication, distribution, etc. | 2003 |
502 ## - DISSERTATION NOTE | |
Degree type | MVSc |
520 3# - SUMMARY, ETC. | |
Summary, etc. | This study was undertaken for finding the molecular genetic diversity among dwarf cattle groups of Kerala namely Vechur, Highrange dwarf, Vatakara and Kasargode using RAPD-PCR technique. Genomic DNA was isolated from 101 animals belonging to the four cattle groups using phenol: chloroform method. The mean yields of DNA isolated from five ml of whole blood was 110.07 ± 9.4 ug and the average ratio between optical densities at 260 and 280 nm was 1.6. The PCR conditions were standardized with respect to annealing temperature (382c), magnesium chloride concentration (1.5 mM) and the amount of Taq DNA polymerase (0.5 units/20 Ill). RAPD-PCR analysis was performed in pooled DNA samples using 26 random oligonucleotides. Individual sample analysis was done by selecting ten primers. The degree of polymorphism obtained for the primers varied from 0 to 100 per cent in different populations. The polymorphism of primers ILO 876, ILO 1127, OPA 20, OPA 12, OPA 09 were higher for all groups tested. ILO 526, OPA 10, OPA 01 and OPA 18 gave moderate levels of polymorphism whereas the polymorphism given by OPA 06 was very low. The primer [LO 1127 produced a distinct band which was present in half of Vechur animals and in one Kasargode cattle. ILO 526 gave a low degree of polymorphism in Kasargode cattle and moderate levels in other groups tested. A predominant population specific band of 2.5 kb was identified in Highrange dwarf cattle with primer OPA 06. When amplified with OPA 20, a band (OPA 20 c) was noticed in Vechur animals with a frequency 0.07 and it was absent in other animals. The primer OPA 18 produced two common bands and the primer OPA 06 produced eight common bands. OPA 014, OPA 09 g and OPA 12 g were the common bands present in all the animals tested when respective primers were used. The band sharing values obtained were higher for Vechur and Vatakara animals (0.83 ± 0.00) and lower for Highrange dwarf and Kasargode animals (0.81 ± 0.00), when within group band sharing was considered. The between group band sharing value varied from 0.80 ± 0.00 (between Highrange dwarf and Kasargode) to 0.83 ± 0.00 (between Vechur and Vatakara). This indicates that Vechur and Vatakara populations are more similar than Highrange dwarf and Kasargode cattle. The least squares analysis of variance of band sharing value showed significant effects (P measure of genetic divergence between groups were not significantly different between Vechur and Vatakara and between Highrange dwarf and Vatakara. They differed significantly between comparisons of Vechur-Highrange dwarf, Vatakara-Kasargode, Vechur-Kasargode and Highrange dwarf-Kasargode combinations. The mean average percentage difference calculated as a measure of genetic dissimilarities between groups, ranged from 17.93 (between Vechur and Vatakara) to 20.92 (between Highrange dwarf and Kasargode). The genetic distance calculated was lowest between Vechur and Vatakara (0.006) and highest between Highrange dwarf and Kasargode (0.046). The dendrogram prepared showed mixing of animals of different genetic groups. In the present study the molecular genetic diversity among four genetic groups of dwarf cattle in Kerala was estimated using the RAPD-PCR technique. It was observed that the Vechur and the Vatakara animals are genetically more similar than the Highrange dwarf and Kasargode animals. |
700 ## - ADDED ENTRY--PERSONAL NAME | |
Personal name | Anilkumar K (Guide) |
856 ## - ELECTRONIC LOCATION AND ACCESS | |
Uniform Resource Identifier | https://krishikosh.egranth.ac.in/handle/1/5810106027 |
942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
Source of classification or shelving scheme | |
Koha item type | Theses |
Withdrawn status | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Permanent Location | Current Location | Shelving location | Date acquired | Full call number | Barcode | Date last seen | Price effective from | Koha item type |
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KAU Central Library, Thrissur | KAU Central Library, Thrissur | Theses | 2014-03-18 | 636.082 SUP/MO | 172184 | 2014-03-18 | 2014-03-18 | Theses |