Effect of brewery waste on nutrient digestability of paddy straw based diet in lactating cows (Record no. 27822)

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003 - CONTROL NUMBER IDENTIFIER
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005 - DATE AND TIME OF LATEST TRANSACTION
control field 20220323110656.0
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082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER
Classification number 636.084
Item number SEN/EF PHD
100 ## - MAIN ENTRY--PERSONAL NAME
Personal name S SenthilKumar
245 ## - TITLE STATEMENT
Title Effect of brewery waste on nutrient digestability of paddy straw based diet in lactating cows
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Place of publication, distribution, etc. Mannuthy
Name of publisher, distributor, etc. Department of Animal Nutrition, College of Veterinary and Animal Sciences
Date of publication, distribution, etc. 2009
300 ## - PHYSICAL DESCRIPTION
Extent 207
502 ## - DISSERTATION NOTE
Degree type PhD
520 3# - SUMMARY, ETC.
Summary, etc. Two experiments were conducted, one to assess the effect of brewery waste on nutrient digestibility of paddy straw based diet in lactating cows and the other to study the in vitro disappearance, degradability and rumen fermentation characteristics of various experimental feeds in Rumen Simulation Technique (RUSITEC).
In animal study, eighteen early lactating cross bred cows within 20 days of lactation were selected and grouped into three as uniform as possible with regard to their age, body weight, parity, previous milk yield and stage of lactation. They were randomly allotted to the three dietary treatments T1, T2 and T3. The control concentrate mixture and paddy straw formed the T1 ration. The animals of T2 and T3 were fed with experimental concentrate mixture containing 25 per cent brewery waste (on DM basis) and paddy straw as roughage. In T2, the brewery waste was fed separately on wet basis, while in T3, the brewery waste to be incorporated in the concentrate mixture was treated with required quantity of paddy straw, three days before feeding. A feeding trial was carried out for a period of 60 days.
There was no significant difference (P>0.05) in body weight and average daily dry matter intake (DMI) of experimental animals among the treatment groups T1, T2 and T3. The animals maintained on the three dietary treatments T1, T2 and T3 showed an average daily milk production of 8.03, 10.14 and 11.09 kg, respectively during the 60 day period of the experiment. The milk production was significantly increased (P<0.05) from fourth week onwards in brewery waste fed groups (T2 and T3) when compared with control (T1). The cost of feed per kg milk produced was lower in animals maintained on T2 and T3 rations (Rs. 7.89 and 8.37, respectively) than those fed control ration (Rs. 10.40).

The brewery waste feeding did not affect milk composition such as percentage of total solids, milk fat, solids not fat (SNF) and milk protein and milk urea nitrogen (MUN). The four per cent FCM yield and total yield of milk fat and protein remained unaffected by brewery waste incorporation. Animals in all the three groups showed similar blood parameters such as haemoglobin, plasma glucose, plasma urea nitrogen (PUN) and plasma calcium and phosphorus. The MUN and PUN concentrations were significantly higher (P<0.05) in T2 at 60th day than that of T1 and T3.
There was no significant difference (P>0.05) in rumen fermentation parameters, such as ruminal pH, NH3-N and TVFA concentration of the animals fed three experimental diets T1, T2 and T3. There was no significant difference (P>0.05) in apparent digestibility of DM, OM, CP, EE, NFE, ADF, cellulose and gross energy among the three treatment rations. Brewery waste fed groups (T2 and T3 ration) recorded significantly higher apparent digestibility of CF and NDF (P<0.05) and hemicellulose (P<0.01) than those fed control diet.
An in vitro experiment was conducted to study the disappearance, degradability and rumen fermentation parameters such as pH, total gas production, CO2 production, NH3-N and TVFA and their fractions such as acetate, propionate, isobutyrate, butyrate and valerate concentration of experimental feeds incubated at different incubation periods in RUSITEC.
The following experimental feeds were incubated in the RUSITEC, 1) control feed; 2) experimental feed (75%) + fresh brewery waste (25%) (EFFBW); 3) experimental feed (75%) + dried brewery waste (25%) (EFDBW); 4) fresh brewery waste (FBW); 5) dried brewery waste (DBW); 6) paddy straw (PS) and 7) brewery waste incorporated paddy straw (BWIPS).
Among the feeds experimented in RUSITEC, disappearance was more in the control feed, EFFBW and EFDBW when compared to other experimental feeds. Similarly, the disappearance was more in BWIPS compared to paddy straw. The in vitro DM, CP, CF, NDF, ADF, hemicellulose and cellulose disappearance rate at all incubation periods were higher for FBW than those for DBW.
The control feed, EFFBW and EFDBW had higher ‘a’ and ‘b’ fractions, rate of degradation ‘c’ and in vitro effective degradability of DM and CP than other experimental feeds such as FBW, DBW, PS and BWIPS and paddy straw showed the lowest. The BWIPS showed higher in vitro effective DM and CP degradability compared to paddy straw. The FBW showed higher DM and CP ‘a’ and ‘b’ fractions, rate of degradation ‘c’ and effective degradability than DBW.
A low in vitro DM and CP disappearance of 33.24 and 30.75 per cent, respectively were recorded at 72 hours of incubation (effective DM and CP degradability of 30.59 and 28.64 per cent, respectively), whereas the BWIPS had improved in vitro DM and CP disappearance of 42.25 and 38.36 per cent, respectively, at 72 hours of incubation (effective DM and CP degradability of 39.93 and 36.32 per cent, respectively).
The data on in vitro rumen fermentation characteristics indicated that during initial incubation periods (2, 6, 12 and 24 hours), the control feed, EFFBW, EFDBW and FBW showed higher in vitro gas production than other experimental feeds, whereas, during later incubation periods (48 and 72 hours), DBW, paddy straw and BWIPS showed higher in vitro gas production than other experimental feeds. Almost similar in vitro CO2 production and pH were recorded at all periods of incubation in seven experimental feeds incubated in RUSITEC. The FBW showed the highest in vitro NH3-N production at all incubation periods, followed by control feed and EFFBW. The paddy straw had the lowest NH3-N production at all incubation periods compared to other experimental feeds. However, the BWIPS showed slightly higher NH3-N production at all incubation periods than paddy straw. The EFFBW and DBW were intermediary NH3-N production at all periods of incubation compared to other experimental feeds in RUSITEC.
The TVFA concentration was comparatively higher in control feed, EFFBW and EFDBW at all periods of incubation than the rest of experimental feeds. The FBW had higher TVFA concentration than DBW in later incubation periods (12, 24, 48 and 72 hours). The EFFBW had higher TVFA concentration during initial incubation periods (2, 6 and 12 hours) than DBW, whereas the DBW had higher TVFA concentration during later incubation periods (24, 48 and 72 hours). During initial incubation periods (2, 6, 12 and 24 hours) all the experimental feeds showed higher acetate concentration than later incubation periods (48 and 72 hours). The EFDBW showed the highest acetate, propionate and iso butyrate concentration (349.64, 266.54 and 90.63 mmol/l, respectively) at 24 hours of incubation, compared to other experimental feeds. The paddy straw and BWIPS showed lower acetate and propionate concentration at all incubation periods.
From the RUSITEC experiment, the effective CP degradability of paddy straw, control concentrate mixture and experimental concentrate mixture (containing 25 per cent brewery waste) were 28.64, 53.97 and 52.37 per cent, respectively. The average daily RUP intake of the animals of three experimental groups T1, T2 and T3 calculated using the effective degradability values were 0.68, 0.71 and 0.72 kg, respectively and the values are higher than the NRC (2001) recommended requirement of 0.50 kg RUP per day for early lactating cows producing 15 kg of milk.
Inclusion of brewery waste at 25 per cent (DM basis) in concentrate mixture in a paddy straw based diet resulted in significantly improved milk production as well as digestibility of crude fibre, NDF and hemicellulose in medium yielding cows in early lactation. The cost of feed per kg milk produced was reduced by 24 per cent by incorporation of brewery waste at 25 per cent level in concentrate mixture (DM basis) in early lactating crossbred cows producing about 10 kg milk per day.
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Viswanathan T V (Guide)
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          KAU Central Library, Thrissur KAU Central Library, Thrissur Theses 2014-03-18 636.084 SEN/EF PHD 172882 2014-03-18 2014-03-18 Theses
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