Characterization and management of viral diseases of black pepper(Piper nigrum L.) (Record no. 27911)
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| 000 -LEADER | |
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| fixed length control field | 03821nam a2200181Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | OSt |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20220325134533.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 140128s9999 xx 000 0 und d |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 632.3 |
| Item number | AYI/CH PHD |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Ayisha R |
| 245 ## - TITLE STATEMENT | |
| Title | Characterization and management of viral diseases of black pepper(Piper nigrum L.) |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc. | Vellayani |
| Name of publisher, distributor, etc. | Department of Plant Pathology, College of Agriculture |
| Date of publication, distribution, etc. | 2010 |
| 502 ## - DISSERTATION NOTE | |
| Degree type | PhD |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | A detailed survey was undertaken to study the occurrence and distribution of viral diseases in black pepper in Thiruvananthapuram and Kollam districts of Kerala. Disease incidence (DI) and per cent disease index (PDI) were determined during survey which showed that per cent DI varied between 0-57 and PDI between 0-18. The disease was prevalent in both the districts. Most of the local cultivars and improved varieties were susceptible to the disease. The characteristics symptoms of disease were chlorotic spots on emerging younger leaves, vein clearing, scattered chlorotic flecks followed by chlorotic mottling along veins leading to interveinal chlorosis and characteristic twisting and curling of leaves. The infected leaves were also observed to be small, crinkled, and brittle with reduced internodal length, leading to typical stunting of plants. Most of the diseased plants were found to be infected with both Cucumber mosaic virus (CMV) and Pepper yellow mottle virus (PYMo V). The presence of these viruses was confirmed through conducting enzyme linked immunosorbent assay (ELISA) on representative samples collected from different locations. The virus was not mechanically transmitted to healthy pepper seedlings. However the virus was found to be transmitted through grafting, insect vectors and also through seeds. The mealy bug, Ferrisia I virgata was found to be the efficient vector although aphid, Toxoptera aurantii, was also found to be transmitting the virus. Thermal inactivation point was recorded at a range of 40-450C and dilution end point between 10-3 and 10-4 for CMV. Host range studies revealed that virus could be readily transmitted to other species in Piperaceae family as well to some of the weed hosts. The virus was partially purified and antiserum was produced with a titre of 1:128. Identification and serological characterization of the virus was done using ELISA and DIBA. Molecular detection of the virus was also performed using PCR and a PCR product of amplicon size 500 bp and 300 bp were obtained for primers specific to CMV and banana streak virus (BSV) respectively. The pathophysiological studies revealed that virus infected plants showed increased phenol, carbohydrate and protein content. The chlorophyll content was found to be less in infected samples. The activity of defence related enzymes like peroxidase, polyphenol oxidase and phenylalanine ammonialyase were found to be more in infected plants. Electrophoretic analysis of virus infected samples through SOS-PAGE revealed the presence of two novel proteins in diseased samples. Analysis of isozymes through native gel revealed the production of an additional isoform of peroxidase and over expression of polyphenol oxidase in infected plants. In screening of varieties for the source of resistance Panniyur, 2, 3 and 4 were found moderately resistant and Karimunda was highly susceptible. Piper colubrinum showed resistance to the virus. Meristem culure attempted was unsuccessful and could not be used as a viable strategy for eliminating the virus infecting black pepper as the meristems were seen contaminated with the pepper badnavirus. |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Joseph P J (Guide) |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | http://krishikosh.egranth.ac.in/handle/1/5810027007 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | |
| Koha item type | Theses |
| Withdrawn status | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Permanent Location | Current Location | Shelving location | Date acquired | Full call number | Barcode | Date last seen | Price effective from | Koha item type |
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| KAU Central Library, Thrissur | KAU Central Library, Thrissur | Theses | 2014-03-18 | 632.3 AYI/CH PHD | 172972 | 2014-03-18 | 2014-03-18 | Theses |