Agrobacterium rhizogenes mediated in vitro multiplication of AMF(Glomus intraradices) in artemisia annua L. (Record no. 27964)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03154nam a2200181Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | OSt |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20220325150048.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 140128s9999 xx 000 0 und d |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 660.6 |
| Item number | THI/AG PG |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Thiagarajan K |
| 245 ## - TITLE STATEMENT | |
| Title | Agrobacterium rhizogenes mediated in vitro multiplication of AMF(Glomus intraradices) in artemisia annua L. |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc. | Vellanikkara |
| Name of publisher, distributor, etc. | Centre for Plant Biotechnology and Molecular Biology, College of Horticulture |
| Date of publication, distribution, etc. | 2010 |
| 502 ## - DISSERTATION NOTE | |
| Degree type | MSc |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Arbuscular mycorrhizal fungi (AMF) are an important biofertiliser and biocontrol agent for agricultural crops. It plays an important role in phosphorous uptake and imparts stress tolerance in plants. At present, large scale multiplication of AMF is done in pots using a suitable host. However, it is difficult to maintain pure culture of AMF in pot culture. Moreover, in vitro multiplication on artificial media is not possible due to its obligate symbiotic nature. One of the recent approaches for the mass multiplication of AMF is the Ri-T-DNA hairy root technique. AMF, if colonised on hairy roots, can be a novel technique for its in vitro mass multiplication. Hence, a study was undertaken to multiply AMF (Glomus intraradices) in the hairy roots of Artemisia annua under in vitro conditions. The studies on the effect of G. intraradices on Artemisia confirmed root colonisation and spores in rhizosphere. Among the two strains of Agrobacterium rhizogenes used for hairy root induction in Artemisia, MTCC 532 was selected and was found to be superior to MTCC 2364. Hairy roots were induced on A. annua as per the procedure already standardised. The cellulase enzyme (0.16 mg l-1) in water agar media enhanced G. intraradices spore germination for inoculation on hairy roots. Normal procedures carried out to infect hairy roots of Artemisia with normal and germinated spores of AMF did not yield positive results. The hairy roots failed to proliferate further and the AMF inoculum also did not survive even after repeated attempts in different culture conditions (solid, liquid static and shaking). However, the possibility of using A. annua as a host for AMF (G. intraradices) was confirmed through pot culture experiments. A. annua based inoculum was found superior to maize based inoculum and it recorded maximum survival per cent, number of leaves, plant height (cm), number of tillers, plant fresh weight and dry weight in pot culture experiment using tissue culture (TC) derived ginger plants. The present study revealed the possibility of using A. annua as a host for multiplication of AMF. However, no root colonisation was observed on hairy roots of A. annua. Microbial contamination during AMF spore inoculation was the main limiting factor for proliferation. Thus, the procedure for in vitro inoculation of AMF spores in hairy roots of A. annua, are yet to be standardised. A suitable medium that will allow survival and proliferation of hairy roots and AMF together also needs to be standardised. |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Surendra Gopal K (Guide) |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | http://krishikosh.egranth.ac.in/handle/1/5810027222 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | |
| Koha item type | Theses |
| Withdrawn status | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Permanent Location | Current Location | Shelving location | Date acquired | Full call number | Barcode | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| KAU Central Library, Thrissur | KAU Central Library, Thrissur | Theses | 2014-03-18 | 660.6 THI/AG PG | 173025 | 2014-03-18 | 2014-03-18 | Theses |