DNA Barcoding of the insetivorous bats of Parambikulam tiger reserve western ghats, Kerala (Record no. 28204)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03509nam a2200181Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | OSt |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20220331162431.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 140128s9999 xx 000 0 und d |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 634.9 |
| Item number | PAR/DN |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Parvathy Venugopal |
| 245 ## - TITLE STATEMENT | |
| Title | DNA Barcoding of the insetivorous bats of Parambikulam tiger reserve western ghats, Kerala |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc. | Vellanikkara |
| Name of publisher, distributor, etc. | Department of Wild life Sciences , College of Forestry |
| Date of publication, distribution, etc. | 2013 |
| 502 ## - DISSERTATION NOTE | |
| Degree type | MSc. |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | The study was carried out at Parambikulam Tiger Reserve during 2012- 2013 with an objective of DNA barcoding the insectivorous bats to unravel the taxonomic ambiguity. The methods employed were the phenol-chloroform extraction (Sambrook et al., 1989) or the GeniPurelM Mammalian Genomic DNA Purification Kit (GeNei™). The sequences were compared with those registered in NeB! databank (blast.ncbi.nlm.nih.gov). The phylogeny reconstruction and the calculation of genetic distances were done using the MEGA 5.0 (Tamura et al., 2011). In this study, three bat species such as Hipposideros speoris, Megederma spasma and Rhinolophus rouxii, were identified using the partial cytochonne b sequence from nine samples of individuals. But the species, Megaderma spasma and Hipposideros speoris showed a genetic distance between two percent and 11 % while compared with the Genbank sequences of those species. Bradly and Baker (2001) considered that a genetic distance more than 10% or between two percent and 11% at cytochrome b is an indication of species level divergence or valid species. So the high sequence divergence in Megaderma spasma and Hipposideros speoris of present study with the same species from South Asia and South India gives an idea on the existence of conspecific population or valid species in these localities. The high sequence divergence may possible as they were collected from two different geographic locations and they result from geographic barriers separating genetic flow. To identify this complexity, the present study recommends additional study concerning specific status and for that more specimens should be collected from each geographical location. Because of this high genetic distance or sequence divergence between the sequences of Megaderma spasma and Hipposideros speoris of present study and that of retrieved from Genbank were clustered as sister clades in their respective phylogenetic neighbour-joining tree. The two sequences of Megaderma spasma, KAUNHM2012318 and KAUNHM2012320, and one sequence of Hipposideros speoris, KAUNHM2012314, were identified as a haplotypes. In the case of Rhinolophus rouxii sequences, the phylogenetic analysis put KAUNHM20114 and KAUNHM201185 together in one branch and they again clustered to the GenBank sequences of 80 kHz phonic type of Rhinolophus rouxii. The genetic distance also confirms this. But the third sequence of Rhinolophus rouxii from the present study, KAUNHM2012310, clustered with the sequences of 90 kHz phonic type of Rhinolophus rouxii and that showed a genetic distance of 9.5% when compared with two other Rhinolophus rouxii sequences of present study. So, the study clearly indicates the existence of sibling species of Rhinolophus rouxii and Rhinolophus indorouxii in the study area and this support the study ofChattopadhyay et al. (2012). |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | P.O.Nameer(Guide) |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | http://krishikosh.egranth.ac.in/handle/1/5810110155 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | |
| Koha item type | Theses |
| Withdrawn status | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Permanent Location | Current Location | Shelving location | Date acquired | Full call number | Barcode | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| KAU Central Library, Thrissur | KAU Central Library, Thrissur | Theses | 2014-03-18 | 634.9 PAR/DN | 173265 | 2014-03-18 | 2014-03-18 | Theses |