Identification of AFLP marker linked with bacterial wilt resistance in chilli (Capsicum annum L) (Record no. 28215)
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| 000 -LEADER | |
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| fixed length control field | 04576nam a2200181Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | OSt |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20220401101041.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 140128s9999 xx 000 0 und d |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 660.6 |
| Item number | THA/IDA |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Thakur Pranita Prabhakarrao |
| 245 ## - TITLE STATEMENT | |
| Title | Identification of AFLP marker linked with bacterial wilt resistance in chilli (Capsicum annum L) |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc. | Vellanikkara |
| Name of publisher, distributor, etc. | Centre for Plant Biotechnology and Molecular Biology, College of Horticulture |
| Date of publication, distribution, etc. | 2013 |
| 502 ## - DISSERTATION NOTE | |
| Degree type | MSc. |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Chilli is one of the most important condiments in India and our country is the largest producer, contributing 25% of the world production. During 2010-11, India produced 0.8 mt of dry chilli from an area of 0.93 mha. Bacterial wilt (caused by Ralstonia solanacearum) is a major reason for the lower productivity of this crop, causing up to complete losses under severe infections. The study entitled “Identification of AFLP marker linked with bacterial wilt resistance in chilli (Capsicum annuum L.)” was carried out at the Centre for Plant Biotechnology and Molecular Biology (CPBMB), College of Horticulture during the period 2011-2013. The objective of the study was to identify AFLP marker linked with bacterial wilt resistance in chilli (Capsicum annuum L.). Three chilli genotypes Ujwala, Anugraha and Pusa Jwala and their progenies were used in the study. Anugraha is a chilli variety with resistance to bacterial wilt disease. Pusa Jwala is the near isogenic line (NIL) of Anugraha, both differing only in the gene for resistance to bacterial wilt. Ujwala was the donor parent for resistance while developing Anugraha from Pusa Jwala through back crossing programme. Pusa Jwala and Anugraha varieties were screened in open field with artificial inoculation to confirm the disease reaction. Highly resistant Anugraha plant was crossed with the pollen from most susceptible Pusa Jwala plant. The F1 seeds were harvested and this generation was field screened to observe the disease reaction. Previous report on bacterial wilt resistance points to monogenic homozygous recessive condition for resistance. Accordingly, all the F1 plants are supposed to be susceptible; but our screening had shown the resistance in F1 generation to be nearly 50 per cent. The reason for the deviation from the expected ratio is attributed to the selection of a heterozygous (Rr) plant as male parent. Further the F1 plants were raised in pots, selfed seeds were harvested and further the pots were infected with bacteria and F2 seeds harvested from susceptible F1s were used for raising the F2 population. F2 population was used as segregating population for generating the susceptible and resistant bulks in bulk segregant analysis (BSA) using AFLP method. Screening of 200 F2 plants was done along with parents Anugraha and Pusa Jwala using leaf cutting and pin pricking methods. F2 segregating plants have shown 69.5 per cent wilt incidence, pointing to ~3:1 ratio, confirming that the resistance is governed by homozygous recessive condition. DNA extraction was done from parents and all the 200 F2 plants and Ujwala by CTAB method (Rogers and Bendich, 1994). Good quality of DNA with UV absorbance ratio (A260/280) ranged 1.8- 2.0 were used for further AFLP analysis. DNA from 9 highly susceptible and 9 highly resistant F2 plants was separately pooled for developing bulk. BSA was carried out with AFLP analysis the DNA using resistant parent (Anugraha), susceptible parent (Pusa Jwala), resistance donor (Ujwala), F2 resistant bulk and F2 susceptible bulk. The AFLP was performed using the standard kit provided by Chromus Biotech, Bangalore, following the restriction digestions using frequent and rare cutters, adapter ligation, pre-amplification and selective amplification using the primer combination EcoACT+ MseCAC. The amplified PCR products were separated by capillary electrophoresis on an ABI Prism 310 Genetic Analyzer along with GeneScan™ 500 LIZ® size standard and data generated were collected by Data Collection 2.1 software. Totally, 124 bands ranging 50-500bp were amplified. Among them, three polymorphic bands with 103, 118 and 161 bp linked with the resistance allele and three polymorphic bands with 183, 296 and 319 bp linked with susceptible allele were identified. Due to the repeatable nature of AFLP, these 6 markers could be directly employed in MAS breeding programmes. |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Deepu Mathew (Guide) |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | http://krishikosh.egranth.ac.in/handle/1/5810111114 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | |
| Koha item type | Theses |
| Withdrawn status | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Permanent Location | Current Location | Shelving location | Date acquired | Full call number | Barcode | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| KAU Central Library, Thrissur | KAU Central Library, Thrissur | Theses | 2014-03-18 | 660.6 THA/IDA | 173276 | 2014-03-18 | 2014-03-18 | Theses |