Characterization of selected Centella asiatica genotypes using asiaticoside and gene-specific markers (Record no. 290261)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02967nam a22001577a 4500 |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 660.6 |
| Item number | JAY/CH PG |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Jayath Gowri J |
| 245 ## - TITLE STATEMENT | |
| Title | Characterization of selected Centella asiatica genotypes using asiaticoside and gene-specific markers |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc | Vellayani |
| Name of publisher, distributor, etc | Department of Plant Biotechnology, College of Agriculture |
| Date of publication, distribution, etc | 2022 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 70p. |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | BSc - MSc (Int.) |
| 520 ## - SUMMARY, ETC. | |
| Abstract | The study entitled “Characterization of selected Centella asiatica genotypes using asiaticoside and gene-specific markers” was carried out at the Biotechnology and Bioinformatics Division of KSCSTE-JNTBGRI, Palode, during 2021-2022 with the objective to characterize six selected accessions of C. asiatica with respect to morphology, asiaticoside production, and gene expression profiling. Morphological characterization was done with the help of eight quantitative traits. The descriptive statistics of the recorded data were performed using Grapes 1.0.0 web application designed by KAU. Five of the eight traits exhibited significant variation among the six accessions. Leaf area, leaf breadth, leaf thickness, petiole length, and petiole diameter revealed significant variation (p<0.05). Quantification using reversed-phase HPLC estimated that CA449 has the highest content of both asiaticoside (ASD) and madecassoside (MSD). The lowest ASD content was found in CA379, while the lowest MSD content was found in CA459. The content of ASD ranged from 4.9- 8.8% per 100g dry weight and MSD ranged from 9.7- 15.3% per 100g dry weight among the six genotypes. Gene expression profiling using real-time PCR revealed that the genes involved in asiaticoside biosynthesis (BAS, SQS, and UGT) show the highest expression in CA449 and the lowest expression in CA379. To understand the statistical relationship between the variables used in the study, correlation analysis was performed with the help of Grapes 1.0.0 web application. ASD was highly positively correlated with leaf length, leaf breadth, leaf area, and petiole length. MSD was found to have a substantial positive correlation with petiole length. The positive correlation between secondary metabolites (ASD & MSD) and gene expression of its biosynthetic genes suggests that changes in phytochemical components could be attributable to genetic variances. The six genotypes have shown significant variation at phenotypic, phytochemical (ASD & MSD) and genetic levels. Best character performance was recorded in the accession CA449 with highest leaf area, leaf length, leaf breadth, petiole length, number of dentations per leaf, high ASD & MSD content and maximum expression of BAS, SQS, and UGT. To summarize, the findings offer an initial assessment of natural variation in selected genotypes of C. asiatica, laying the groundwork for future breeding purposes. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Plant Biotechnology |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | K K Sabu (Guide) |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | |
| Item type | Theses |
| Not for loan | Collection code | Permanent location | Current location | Shelving location | Date acquired | Full call number | Barcode | Date last seen | Koha item type |
|---|---|---|---|---|---|---|---|---|---|
| Not For Loan | Reference Book | KAU Central Library, Thrissur | KAU Central Library, Thrissur | Theses | 2023-05-05 | 660.6 JAY/CH PG | 175682 | 2023-05-05 | Theses |