Effect of chitosan application on physiological, biochemical and molecular characteristics of Piper longum L. (Record no. 290266)
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000 -LEADER | |
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fixed length control field | 05779nam a22001697a 4500 |
082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
Classification number | 660.6 |
Item number | ABH/EF PG |
100 ## - MAIN ENTRY--PERSONAL NAME | |
Personal name | Abhijith K |
245 ## - TITLE STATEMENT | |
Title | Effect of chitosan application on physiological, biochemical and molecular characteristics of Piper longum L. |
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
Place of publication, distribution, etc | Vellayani |
Name of publisher, distributor, etc | Department of Plant Biotechnology, College of Agriculture |
Date of publication, distribution, etc | 2022 |
300 ## - PHYSICAL DESCRIPTION | |
Extent | 105p. |
502 ## - DISSERTATION NOTE | |
Dissertation note | BSc - MSc (Int.) |
520 ## - SUMMARY, ETC. | |
Abstract | The study on the "Effect of chitosan application on physiological, biochemical and molecular characteristics of Piper longum L." was carried out in the Department of Plant Biotechnology and Department of Plantation Crops and Spices, College of Agriculture, Vellayani, Thiruvananthapuram, Kerala during 2021-2022. The objective of the study was to investigate the physiological, biochemical and molecular responses of Piper longum to foliar application of chitosan (CHT). The plants raised from the rooted cuttings of long pepper variety, Viswam were exposed to foliar spray with varying concentrations of chitosan (1 g L-1 , 2 g L-1 , 3 g L-1 , 4 g L-1 ) at 2, 4, and 6 months after planting (MAP). The control plants were devoid of chitosan application. The study was carried out in a completely randomized design (CRD) with four replications. The observations were taken on plant growth, physiological and biochemical parameters and molecular characteristics. The plant growth parameters recorded at 3, 5 and 7 MAP, showed significant variation among the treatments tried. CHT 1 g L-1 recorded significantly higher values for the growth parameters at all stages of observation. At 7 MAP, the foliar treatment with CHT 1 g L-1 recorded the highest values for plant height (194.75 cm), number of primary branches (7.75), number of spike-bearing branches (8.5), days to emergence of the spike (73.78 days), days to flowering (20.40 days) and days from emergence to maturity of the spike (59.83 days). The physiological parameters at 3, 5, and 7 MAP, showed significant variation among the chitosan foliar spray treatments. The data on physiological parameters showed a similar trend at all stages of observation with CHT 1 g L-1 , recording the highest values. At 7 MAP, CHT 1 g L-1 showed significantly higher values (496.50 m moles m-2 s -1 , 247.75 mm-2 , 8.87 µmol CO2 m-2 s -1 , and 6.83 mmol CO2 mol-1 H2O, respectively) for stomatal conductance, stomatal distribution, photosynthetic rate and water use efficiency. With respect to enzyme activity, the highest peroxidase (19.86 activity g-1 min-1 ) and 103 catalase (933.48 activity g-1 min-1 ) activities were observed at 7 MAP, in plants subjected to chitosan foliar spray with CHT 1 g L-1 . At 7 MAP, the highest value (1.87 mg g-1 ) of chlorophyll content was also observed in the treatment with CHT 1 g L-1 . The oleoresin, volatile oil and piperine contents obtained from mature dark green dried and powdered spike showed significant differences among the chitosan foliar spray treatments tried. The foliar spray with CHT 1 g L-1 gave significantly higher values with respect to oleoresin (10.70 per cent), piperine (1.34 per cent) and volatile oil (1.46 per cent) content. The yield parameters viz., the number of spikes per plant, girth and length of the spike, spike yield per plant and root yield per plant significantly varied with respect to various chitosan foliar spray treatments. Treatment with CHT 1 g L-1 was observed to be significantly superior with respect to the number of spikes per plant (181.5), girth of the spike (9.997 mm), length of the spike (3.25 mm), spike yield (fresh-163.35 g plant-1 ; dry31.81g plant-1 ) and root yield (fresh- 47.08g plant-1 ; dry-8.24g plant-1 ), among all the treatments tried. The driage however, did not show any significant variation among the treatments. The molecular analysis of Piper longum was done using the leaf samples collected 24 h after the application of foliar spray at 4 MAP. RNA isolated from the sample was used for cDNA synthesis followed by RT-qPCR. The relative fold change analyzed using Ct values showed upregulation of the piperine synthase gene in treatment CHT 1 g L-1 (1.87 fold) and CHT 2 g L-1 (1.34 fold) with respect to control treatment. The chitosan foliar spray at higher concentrations of CHT (3 g L-1 and 4 g L-1 ) was observed to be inhibitory, giving a lower performance, even lower than the control treatment, with respect to the various parameters studied. The study confirmed that foliar spray of CHT at concentrations 1 g L-1 and 2 g L-1 , at 2, 4 and 6 MAP could effectively enhance plant growth, physiological, biochemical, molecular, and yield characteristics. However, the best performance was observed in the 104 lowest concentration of chitosan tried, 1 g L-1 . The foliar application of CHT 1 g L -1 gave maximum enhancement in piperine content, oleoresin, volatile oil, spike yield, and root yield with an increase of 37.46, 25.86, 5.77, 21.72, and 31.6 per cent, respectively over the control treatment. The molecular basis of increase in piperine content was studied by RT-PCR using piperine synthase primer showed upregulation of piperine synthase gene under CHT application at 1 g L-1 . The results of the study indicated that chitosan, a non-toxic and ecofriendly biopolymer has the potential for substantial enhancement of yield and quality of the commercially exploited medicinal plant, Piper longum. The mechanism of action of chitosan to this effect is yet to be clearly elucidated. |
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
Topical term or geographic name as entry element | Plant Biotechnology |
700 ## - ADDED ENTRY--PERSONAL NAME | |
Personal name | Deepa S Nair (Guide) |
856 ## - ELECTRONIC LOCATION AND ACCESS | |
Uniform Resource Identifier | https://krishikosh.egranth.ac.in/handle/1/5810208245 |
942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
Source of classification or shelving scheme | |
Item type | Theses |
Not for loan | Collection code | Permanent location | Current location | Shelving location | Date acquired | Full call number | Barcode | Date last seen | Koha item type |
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Not For Loan | Reference Book | KAU Central Library, Thrissur | KAU Central Library, Thrissur | Theses | 2023-05-05 | 660.6 ABH/EF PG | 175677 | 2023-05-05 | Theses |