Seed characterization and standardization of in vitro seed germination protocol in Rhynchostylis retusa (L.) Blume. (Record no. 290625)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 05445nam a22001937a 4500 |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 630.28 |
| Item number | AKH/SE PG |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Akhila Rajan |
| 245 ## - TITLE STATEMENT | |
| Title | Seed characterization and standardization of in vitro seed germination protocol in Rhynchostylis retusa (L.) Blume. |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc | Vellayani |
| Name of publisher, distributor, etc | Department of Plant Breeding and Genetics, College of Agriculture |
| Date of publication, distribution, etc | 2023 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 63p. |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | MSc |
| 520 3# - SUMMARY, ETC. | |
| Abstract | The present study entitled “Seed characterization and standardization of in vitro seed germination protocol in Rhynchostylis retusa (L.) Blume.” was carried out in the Department of Plant Breeding and Genetics, College of Agriculture, Vellayani during the year 2020-22. The experiment was aimed at seed characterization and standardization of asymbiotic seed germination protocol in Rhynchostylis retusa (L.) Blume. Germplasm repository maintained at Saraswathy Thangavelu Extension Centre of JNTBGRI, Puthenthope, Thiruvananthapuram was utilized to observe the flower characters and the capsules for the characterization and in vitro seed germination study. The research work was divided into four experiments. Experiment I - evaluation of floral characters, experiment II -morphological and morphometric characterization of seeds, experiment III- preparation of in vitro seed germination media, and experiment IV -in vitro seed germination in culture media and evaluation. All the experiments were carried out in five replicates and mean was calculated. In the first experiment, various floral, pollen and capsule characters were noted and qualitative characters were expressed descriptively after observing the plants behaviour closely and quantitative characters were assessed and mean was worked out. Rhynchostylis retusa was seasonal (June-August) in flowering behaviour. Two types of plants were observed, one with white flowers having purple spots or tessellation and other with blush of purple on white background. Anthesis was between late evening and early morning. The stigma was receptive in young flowers until five days after anthesis and after which the stigma become dried out. Morning hours until 10.30 am showed maximum receptivity than other times of the day. Under natural conditions capsule setting was noticed within seven days of flowering. Morphology of pollinia was observed visually. They were crushed and stained using acetocarmine to understand the individual pollen structure, number, arrangement, viability etc. The pollen load per pollinium estimated by haemocytometer averaged to 2,06,000. Furthermore, almost all the pollen was found to be viable and 73.64% of them germinated in sucrose solution of 10 per cent strength. The fruit or capsule at maturity was 2.18 cm long and 0.86 cm wide. Undehisced mature capsules of 145-180 days of pollination was found to be best for in vitro culture. 67 In experiment II, seeds of R. retusa was characterized morphologically under compound light microscope and morphometric data analysed using microscopic software package Leica LAZ 500. The seeds were fusiform shaped, elongated, with prolate spheroid embryo of violet colour. The testa cells were yellowish white and reticulated. At maturity 78.74 percent of the seed volume was filled with air space. The viability of seeds used for seed culture was worked out to be 78.87 percentage. Out of the millions of seeds in a capsule 41.87 percentage of seeds were found to be well filled with large embryo of regular shape. In experiment III- for the in vitro seed germination studies four different media viz., MS, half-strength MS, Knudson C orchid media and WPM including liquid and solid with and without growth hormones were prepared and used. The seeds from mature disinfected capsules were scooped out and inoculated under sterile conditions. Five replicates of each media was inoculated with seeds. Observations were recorded on the number of days for showing seed greening and chlorophyll embryo, for protocorm development, for number of protocorms produced and number of protocorms with shoot apex and multiple shoots, number of days for root and leaf initiation. Half MS medium (liquid) was observed to be the best among the different media used for asymbiotic seed germination of Rhynchostylis retusa. Half MS was best media for greening and protocorm development. If we compare the overall response for different stages of development half MS with 0.5 mgL-1 BAP (solid) was found best for callusing and shoot proliferation. Half MS with 0.5 mgL-1 BAP and 1 mgL-1 NAA (solid) for multiple shoots, small leaves and root development. Initiation of germination was seen within two months of culture inoculation. The grain shaped protocorm needed to be subcultured at proper time and calloid like structures with multiple shoots could be divided to separate units and each unit developed to separate plantlets. Seed characterization provides an insight into understanding biology, ecology and evolutionary background. Mass multiplication by asymbiotic seed culture can be a viable option for restoring this threatened species and can be utilized for further genetic improvement programme. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Plant breeding and genetics |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Vitro seed |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | germination protocol |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Rhynchostylis retusa (L.) blume |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Seeja G (Guide) |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | |
| Item type | Theses |
| Not for loan | Collection code | Permanent location | Current location | Shelving location | Date acquired | Full call number | Barcode | Date last seen | Koha item type |
|---|---|---|---|---|---|---|---|---|---|
| Not For Loan | Thesis | KAU Central Library, Thrissur | KAU Central Library, Thrissur | Theses | 2023-09-25 | 630.28 AKH/SE PG | 175838 | 2023-09-25 | Theses |