Molecular characterization of brinjal (Solanum melongena L.) genotypes and their performance assessment in summer (Record no. 290636)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 04882nam a22002057a 4500 |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 630.28 |
| Item number | CHA/MO PG |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Chakravaram Alekhya |
| 245 ## - TITLE STATEMENT | |
| Title | Molecular characterization of brinjal (Solanum melongena L.) genotypes and their performance assessment in summer |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc | Padanakkad |
| Name of publisher, distributor, etc | Department of Genetics and Plant Breeding, College of Agriculture |
| Date of publication, distribution, etc | 2023 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | xiii, 150p. |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | MSc |
| 520 3# - SUMMARY, ETC. | |
| Abstract | The study entitled “Molecular characterization of brinjal (Solanum melongena L.) genotypes and their performance assessment in summer” was conducted at the Department of Plant Breeding and Genetics, College of Agriculture, Padannakkad, Kasaragod during 2020-2022. The main objectives of the present study were to assess the performance of brinjal genotypes in summer season and to characterize them using RAPD, ISSR and SSR markers. The experimental material consisted of 30 accessions which included 11 collections from North Kerala, 16 accessions from NBPGR regional station, Thrissur and three KAU-released varieties (Ponny, Haritha and Surya). The experiment was laid out in a randomized block design with 30 genotypes in three replications and observations were recorded for 26 morphological characters. The results of analysis of variance revealed significant differences for all the characters indicating high genetic variability among the accessions. The genotypes SM-9, SM-19 and SM-14 showed superiority for fruit yield per plant (3204.73 g, 3017.90 g, 2484.50 g), fruits per plant (41.67, 36.67 and 53.33) and marketable fruit yield (3138.37 g, 2946.17 g, 2450.13 g). Correlation and path analysis revealed total number of fruits, fruit width and single fruit weight displayed a positive significant correlation and positive direct effect with fruit yield per plant explaining a true association of these traits with yield. The PCA biplot showed marketable fruit yield, single fruit weight and total fruits per plant displayed greater phenotypic contribution towards yield. SM-19, SM-12 and SM-10 are found superior due to their close proximity to the yield and its contributing vectors, while SM-9 and SM-14 were found to be more diverse among all the genotypes. The genetic diversity analysis based on Mahalanobis D2 statistics for 22 morphological traits grouped the thirty accessions into nine clusters. The character single fruit weight contributed maximum towards diversity. The highest intracluster distance was recorded in cluster I followed by cluster IV. The maximum inter-cluster distance was recorded between clusters IV and IX followed by clusters IV and VIII exhibiting a wide diversity between these clusters providing 146 scope for hybridization between superior genotypes of these clusters. The accessions SM-10, SM-15, SM-9 and SM-19 showed superior ranking in their respective clusters based on three yield attributes total fruits per plant, single fruit weight and marketable fruit yield identified in association and divergence analysis. The 30 brinjal genotypes were characterized using RAPD, ISSR and SSR markers. The molecular investigation with 15 RAPD markers amplified 48 effective alleles. The highest value for number of effective alleles (4.83), PIC (0.80) and marker index (2.86) was revealed for OPC10. Based on UPGMA-based Jaccard’s similarity coefficient the 30 accessions divided into three major groups at 0.72 similarity. Six (out of 10) ISSR markers amplified 24 effective alleles. The highest value for number of effective alleles (5.25), PIC (0.857) and marker index (4.031) was revealed for UBC888. UPGMA clustering divided the 30 accessions into 3 clusters at 0.78 similarity. The ten SSR markers amplified 17 effective alleles. The highest value for number of effective alleles (1.99), PIC (0.374) and marker index (0.511) was revealed for EEMS24. Based on UPGMA clustering the 30 accessions divided into three major groups at 0.61 similarity. The dendrogram (UPGMA) of pooled data yielded three major clusters at 0.76 similarity. The PCA clustering of pooled data displayed SM-14 and S.incanum to be diverse among all the accessions. The present study resulted in identification of high-yielding and diverse lines amongst the collection of the brinjal genotypes used. Based on superior performance for yield in summer season and pooled molecular divergence analysis, the melongena accessions SM-2, SM-9, SM-10, SM-12, SM-14 and SM19 were found to be superior and can be considered as promising parents in future crossing programs to develop heterotic hybrids. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Genetics |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Plant breeding |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Brinjal |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Solanum melongena L. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Molecular characterization |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Namboodiri Raji Vasudevan (Guide) |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | |
| Item type | Theses |
| Not for loan | Collection code | Permanent location | Current location | Shelving location | Date acquired | Full call number | Barcode | Date last seen | Koha item type |
|---|---|---|---|---|---|---|---|---|---|
| Not For Loan | Thesis | KAU Central Library, Thrissur | KAU Central Library, Thrissur | Theses | 2023-09-25 | 630.28 CHA/MO PG | 175833 | 2023-09-25 | Theses |