Suitability of Pleurotus ostreatus (DMRP-30) for cultivation in Kerala (Record no. 290674)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 04868nam a22001937a 4500 |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 632.3 |
| Item number | AME/SU PG |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Amelinora Tariang |
| 245 ## - TITLE STATEMENT | |
| Title | Suitability of Pleurotus ostreatus (DMRP-30) for cultivation in Kerala |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc | Vellayani |
| Name of publisher, distributor, etc | Department of Plant Pathology, College of Agriculture |
| Date of publication, distribution, etc | 2023 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 104p. |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | MSc |
| 520 3# - SUMMARY, ETC. | |
| Abstract | The present study entitled “Suitability of Pleurotus ostreatus (DMRP-30) for cultivation in Kerala” was conducted at AICRP on Mushroom Laboratory, Department of Plant Pathology, College of Agriculture, Vellayani during 2020-2022 with an objective to standardize the spawn production and substrate for the unexploited Pleurotus ostreatus (DMRP-30). The culture was initially procured from ICAR-DMR (Directorate of Mushroom Research), Solan and used for spawn preparation followed by cultivation on paddy straw. Morphological characterisation of P. ostreatus (DMRP-30) revealed that the sporocarps, were white, moderate to big oyster shaped (7.87 cm x 5.32 cm) with eccentric, slightly pubescent stipe (3.85 cm thick). The spore print was white. The studies on microscopic characters disclosed that hypae (2.32 μm width) was hyaline, septate, with numerous clamp connections. The basidia (21.48 μm x 6.11 μm) were clavate with tetra sterigmata and four smooth elliptical basidiospores (8.85 μm x 4.46 μm). Cultural and physiological studies showed that potato dextrose agar was the best medium for mycelial growth of P. ostreatus with a growth rate of 1.11 cm day-1 and took minimum period for completion of growth in Petri dish (9 days). The optimum temperature and pH was identified as 30 ℃ and 7-7.5 respectively. The lower temperature of 20 ℃ and acidic pH of 4 retarded the mycelial growth. Maximum mycelial biomass was obtained from malt extract broth and oatmeal broth at 21 and 30 DAI respectively (0.6 and 0.8 g mycelial dry weight). Among various spawn substrate (grains) viz., paddy, wheat, sorghum, maize and ragi, paddy was found to be the best spawn base with a thick fluffy mycelial growth, minimum days for complete mycelial colonization (18 days) and minimum contaminants. The total yield from beds prepared with paddy grains spawn was also higher (644.67 g kg-1 of substrate) with out any contaminants compared to sorghum grains which was on par. Evaluation of various substrates viz., paddy straw, rubber saw dust, coir pith amended with rice and wheat bran (10 %) and neopeat for production of P. ostreatus in two seasons showed paddy straw as the optimal substrate with a maximum yield of 585.60 g (April) and 761.50 g (September) from three harvests. The sporocarp production in September took minimum period with bigger sporocarps and enhanced yield when compared to April. The maximum number (63.0) and weight of sporocarps (7.80 g) was recorded from mushrooms harvested from paddy straw substrate followed by neopeat and sawdust. The coirpith amended with wheat bran and rice bran beds failed to produce sporocarps due to heavy contamination with Trichoderma spp. Pest like sciarid flies, phorid flies, staphylinid beetles and slugs were predominant during the sporocarp formation stage in the different substrates used. The major fungal contaminants observed were Trichoderma spp. and Coprinus spp. The proximate analysis of P. ostreatus revealed the constituents including moisture (83.50 %) carbohydrate (38 %), protein (13 %), fibre (5.16 %) and fat (2.17 %). The organoleptic characters of P. ostreatus was compared to P. florida and H. ulmarius where sensory rating scores for appearance, colour, flavour, texture and taste were done. P. ostreatus was found to be highly acceptable and preferred with 40 per cent evaluators liking it very much compared to P. florida (30 %) and H. ulmarius (20 %). Mushrooms stored in refrigerated condition (15 ℃) had better shelf life than those stored at room temperature (28 ± 2 C). The packaging material (polypropylene – 0.20” thickness) enhanced the keeping quality of P. ostreatus both at room temperature and refrigerated condition compared to polyethylene ( 0.15” ). Maximum shelf life (4 days) was observed when sporocarps were packed in perforated polypropylene and kept in regulated condition at 15 ℃. The study indicated that the strain DMRP - 30 of P. ostreatus can be successfully cultivated in Kerala using the locally available substrates for spawn production and cultivation. Good yield, proximate constituents and better shelf life could contribute to its consumer and market acceptability in the state. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Plant pathology |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Pleurotus ostreatus (DMRP-30) |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Cultivation |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Sporophore characters |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Heera G (Guide) |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | |
| Item type | Theses |
| Not for loan | Collection code | Permanent location | Current location | Shelving location | Date acquired | Full call number | Barcode | Date last seen | Koha item type |
|---|---|---|---|---|---|---|---|---|---|
| Not For Loan | Thesis | KAU Central Library, Thrissur | KAU Central Library, Thrissur | Theses | 2023-10-04 | 632.3 AME/SU PG | 175817 | 2023-10-04 | Theses |