Induction of embryogenic calli from immature ovaries in coconut (cocos nucifera L.) (Record no. 290917)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03253nam a22002297a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | OSt |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 660.6 |
| Item number | VYS/IN P G |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Vyshnavi, K |
| 245 ## - TITLE STATEMENT | |
| Title | Induction of embryogenic calli from immature ovaries in coconut (cocos nucifera L.) |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc | Vellayani |
| Name of publisher, distributor, etc | Department of Molecular biology and biotechnology , College of Agriculture |
| Date of publication, distribution, etc | 2023 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 69p. |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | MSc |
| 520 3# - SUMMARY, ETC. | |
| Abstract | The study entitled " Induction of embryogenic calli from immature ovaries in coconut (Cocos nucifera L.)" was conducted at the Department of Molecular Biology and Biotechnology, College of Agriculture, Vellayani, Thiruvananthapuram during 2022-23. The objective of the study was induction of embryogenic calli from immature ovary in coconut variety West Coast Tall (WCT) and effect of sodium butyrate in callus induction through histone deacetylation. In the present study, immature ovaries from explants of maturity indices - 4 to -6 were extracted and surface sterilized using 3% sodium hypochlorite, 0.2% mercuric chloride, and 70% ethanol. They were inoculated into basal Y3 medium containing different plant growth regulators such as 2,4-D, TDZ, and picloram. The results showed that out of all the maturity indices tried (-4 to -6), inflorescence of maturity index -4 showed a maximum response in callus induction, and among different callus induction medium (CIM) tried CIM with 100 μM 2,4-D showed maximum response (47.28%) to embryogenic calli initiation. The time taken for embryogenic calli-like structure development was 45 days. To analyze the effect of sodium butyrate (SB) in callus initiation, the excised immature ovaries were inoculated into standardized CIM (Y3 + 100 μM 2,4-D) with different concentrations of SB. The results displayed that, compared with the CIM, the induction rates of embryogenic calli were significantly increased (66%) and the time taken for embryogenic calli-like structure development was significantly reduced (20 days) with the addition of 100 μM SB. The gene expression analysis shows that gene expression of the HDA6 gene was reduced in CIM with SB when compared to CIM. The increased embryogenic response of immature ovaries to CIM with SB as well as the reduced time taken for the embryogenic response can be correlated with the downregulation of the HDAC gene. To conclude, induction of embryogenic calli and the time taken for embryogenic response of immature ovary in coconut variety West Coast Tall (WCT) has improved by adding SB in CIM. This may be due to the downregulation of the HDAC gene. Reduced activity of histone deacetylase can accumulate acetylated histone and thereby increase gene expression. The preliminary data showed that SB at a lower concentration (100 μM) can improve the embryogenic calli response of immature ovaries of coconut in callus induction medium. The mechanism of action of SB on coconut embryogenesis and the regulated genes must be investigated further |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Molecular biology |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Biotechnology |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Cocos nucifera |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Embryogenic calli |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Anuradha, T (Guide) |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | |
| Item type | Books (General) |
| 500 ## - GENERAL NOTE | |
| General note | B.SC-M.SC |
| 546 ## - LANGUAGE NOTE | |
| Language note | English |
| Not for loan | Collection code | Permanent location | Current location | Shelving location | Date acquired | Full call number | Barcode | Date last seen | Koha item type |
|---|---|---|---|---|---|---|---|---|---|
| Not For Loan | Thesis | KAU Central Library, Thrissur | KAU Central Library, Thrissur | Theses | 2024-02-01 | 660.6 VYS/IN P G | 175898 | 2024-02-01 | Theses |