Mass queen rearig and royal jelly characterization in Indian bee, Apis cerana indica fab (Record no. 291403)

000 -LEADER
fixed length control field 05240nam a22001817a 4500
082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER
Classification number 632.6
Item number ANJ/MA PG
100 ## - MAIN ENTRY--PERSONAL NAME
Personal name Anjali, J S
245 ## - TITLE STATEMENT
Title Mass queen rearig and royal jelly characterization in Indian bee, Apis cerana indica fab
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Place of publication, distribution, etc Vellayani
Name of publisher, distributor, etc Department of Entomology, College of Agriculture
Date of publication, distribution, etc 2023
300 ## - PHYSICAL DESCRIPTION
Extent x,63p.
502 ## - DISSERTATION NOTE
Dissertation note MSc
520 3# - SUMMARY, ETC.
Abstract The study entitled “Mass queen rearing and royal jelly characterisation in Indian bee Apis cerana indica Fab.” was carried out at Department of Entomology, College of Agriculture, Vellayani during 2021-2023. The objectives of the study were standardisation of mass queen rearing and characterization of the royal jelly in Indian bee, A. c. indica.
Ten good Indian bee colonies (C1 to C10) maintained at AICRP on Honey bees and Pollinators were selected based on the previous record. The colonies were screened for their economic traits (honey and pollen area in the brood), colony development traits (brood area, number of brood cells, number of combs containing brood, brood survival rate ie., number of eggs hatched and bee population), hygienic traits (number of pin-killed pupae removed, brood pattern continuity and cleanliness of the bottom board) and foraging behaviour (number of incoming bees and outcoming bees per min during different times of a day). Observations were taken during the honey flow season (March-April), dearth season (May-July) and brood rearing season (Aug-Oct). A non-uniform trend was found among colonies in different parameters. Significant difference was observed in traits like honey area, brood population and brood survival rate during the honey flow season, where the colony C7 had the highest honey area (168.25cm2) and brood area (620cm2) while the colony, C1 exhibited highest brood survival rate (97.75%), number of combs occupied by brood (4.00) and foraging activity (57.50 bees/min). C1 was found to be the best colony in terms of honey area (67.00cm2), brood survival rate (96.50%), brood area (576.36cm2), number of brood cells (2305.45), pin killed pupae removed (100%), brood pattern (1.67), number of combs occupied by brood (3.77) and foraging activity (40.50bees/min) during the dearth season. The performance of the colonies varied during the brood rearing season.
The parameters (honey, pollen and brood area) which exhibited significant variation among the colonies were further analysed to assess their monthly variation throughout the seasons. It was found that highest honey storage was recorded during March (90.50cm2) followed by April (78.05cm2), while the pollen area was at its peak during May to June which ranged from 32cm2 to 33 cm2. Brood area was found highest during August (525cm2) followed by March (482.50 cm2)
Correlation analysis of traits which had prominent role on colony development viz., honey area, pollen area, brood area, bee population and foraging activities with the weather parameters revealed a significant negative correlation with rainfall and relative humidity whereas a positive correlation was found with sunshine hours. Pollen area had a significant positive correlation with the temperature. With regard to the foraging activity, it showed a significant positive relation with temperature and sunshine hours.
Based on the selection index, the colonies were ranked and the first five colonies which showed good performance (C1, C7, C4, C6 and C8) were selected for raising the next generation by the Doolittle method of grafting. Comparative analysis of traits between the parent and daughter colonies revealed that no significant difference exists among the colonies, thus they exhibited a similar performance. An exception was found in case of pollen area where daughter colonies had more pollen area than the parent colonies.
The number of queens which can be reared at a time was assessed by Doolittle method of grafting. For this, queen less colonies with population levels of six frame in the brood chamber was utilized. Percentage acceptance of queen cells was found to be higher in colonies provided with 10 grafts when compared to five and 20 grafts. Monthwise queen cell acceptance showed highest percentage (66.66) in August.
The royal jelly which has a major role in mass queen rearing was analysed for the major constituents based as per standard protocols. Analysis of major components in royal jelly revealed protein (35.70%) as the major component followed by lipid (20.00%). Among vitamins, vitamin B2 (15.30%) was found in significant proportion.
Thus, the present study concludes that the quality traits of Indian bee mother colonies can be transferred to the daughter colonies through Doolittle method of grafting. The optimum number of grafted queen cells for mass queen rearing was standardised as ten with the peak queen cell acceptance during August. The analysis of major components in Indian bee royal jelly revealed the presence of protein (35.70%), lipid (20.00%) and vitamins (20.61%).
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Entomology
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Indian bee
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Apis cerana indica fab
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Amritha, V S(Guide)
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme
Item type Theses
Holdings
Not for loan Collection code Permanent location Current location Shelving location Date acquired Full call number Barcode Date last seen Koha item type
Not For Loan Thesis KAU Central Library, Thrissur KAU Central Library, Thrissur Theses 2024-07-06 632.6 ANJ/MA PG 176097 2024-07-06 Theses
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