MSc

Cowpea (Vigna unguiculata L. Walp) is one of the most important legume crops grown in India. It is a versatile pulse crop owing to its nutritional value, weed smothering nature, drought tolerant characters, soil restoring properties and multi-purpose uses. Protein deficiency has been reported to be one of the main nutritional problems in the developing world. About one billion people are reported to be suffering from protein deficiency and malnutrition worldwide.
Studies have revealed that protein content in cowpea grain ranges between 18 to 40 per cent depending on the genotypes. Identifying high yielding varieties of cowpea with high protein content will not only contribute to food security and alleviate poverty but could also contribute to the alleviation of protein deficiencies. Review on the earlier research pointed to poor emphasis on the genetic improvement of cowpea for improved protein content. Hence, the present study was envisaged to combine the high protein trait with high yielding genotypes.
The study was grouped under three experiments, i) Evaluation of cowpea genotypes, ii) Hybridisation of the selected genotypes in line x tester design, iii) Evaluation of F1 hybrids. The experiments were conducted at College of Horticulture, Kerala Agricultural University, Vellanikkara during 2014-2015. All the crop management practices were followed as per KAU (2011).
Evaluation of cowpea genotypes revealed that there was wide variability for all the traits studied except branches per plant among bushy and semi trailing types of cowpea. In trailing type, variability was observed for plant height, number of pods per plant, pod weight grain yield per plant and seed protein content. Among the twenty two genotypes evaluated, ten genotypes that exhibited a protein content of above 25 per cent were selected for hybridization programme. The selected genotypes
(Vellayani Jyothika, Bhagyalakshmi, Anaswara, Kanakamony, Lola, Vyjayanthi, AV-5, PKB-3, PKB-4 and Sharika) were hybridized in line x tester (6 x 4) design.
High magnitude of phenotypic coefficient of variation (PCV), genotypic coefficient of variation (GCV), heritability and genetic advance was observed for plant height, grain yield per plant and length of pods were observed in parents and hybrids suggesting scope for genetic improvement of these traits through selection. Seeds per pod and protein content exhibited low PCV and GCV but high heritability and low genetic gain, indicating that these traits were governed by non-additive gene action and therefore breeding method other than simple selection is required.
Combining ability analysis revealed that higher magnitude of specific combining ability (SCA) variances than the general combining ability (GCA) variances for the traits like pods per plant, length of pod, test weight, grain yield per plant and seed protein content indicating preponderance of non-additive gene action in the inheritance of these traits. Higher estimates of GCA variances over SCA variances for plant height, days to flowering, days to first harvest, days to last harvest, pod-weight and seeds per pod implies the preponderance of additive gene action in controlling these traits.
The hybrids were ranked based on mean value, sca effects and estimates of heterosis for the traits like days to flowering, pods per plant, seeds per pod, test weight, grain yield per plant and protein content. Four hybrids with the lowest scores were selected. Hybrids H2 (Vellayani Jyothika x PKB-3), H10 (Anaswara x PKB-3), H11(Anaswara x PKB-4) and H12 (Anaswara xSharika) were found to be the most promising. The transgressive segregants with high yield and protein content can be identified on further evaluation of the segregating population from the above hybrids