MSc

A study intended for “Characterization of soil and water of Palakkad eastern plains in relation to growth and nitrogenase content of Azolla spp.” was undertaken in the Department of Soil Science &Agricultural Chemistry, College of Horticulture, KAU, Vellanikkara during 2013-2015. The objectives of the study were to conduct a survey of Azolla spp. in the rice growing tracts of Palakkad eastern plains and to identify soil and water quality parameters congenial for the growth and nitrogenase content of Azolla spp.
The preliminary survey conducted in the four block panchayaths of Palakkad eastern plains to identify the prevalence of Azolla revealed two blocks viz. Chittoor and Kollengode to be positive with respect to Azolla and hence, further study was restricted to the grama panchayaths of these blocks.
Characterization of soil and water was done in both Azolla growing and non-growing regions. Composition of Azolla collected from different locations was also determined. In order to ascertain the nitrogenase enzyme activity, samples of Azolla collected from five different locations were subjected to Acetylene Reduction Assay (ARA).
Among the soil parameters, significant difference was noticed between Azolla growing and non-growing locations with respect to pH , EC, organic carbon, available N, P , total Fe, Mn and Zn in contrast to potassium and copper which did not show any pronounced variation. Soil pH, EC, available P, total Fe, Mn and Zn were comparatively lower in Azolla growing regions. However, available nitrogen in soil was more in Azolla growing locations. Heavy metals like Cd, Cr, Ni and Pb were below the detectable limits in soil.
Analysis on flood water quality showed significant effect in the Azolla growing regions on parameters like pH, temperature and dissolved oxygen, Fe, Mn, Zn and Cu. Soil analysis data revealed the pH, EC, soluble Fe, Mn, Zn and Cu to be lower under Azolla growing conditions. Heavy metals like Cd, Cr, Ni
and Pb were below the detectable limits. Presence of Azolla decreased the flood water temperature but increased the dissolved oxygen content.
On comparison, the composition of Azolla revealed a prominent and significant correlation with location on its content of carbon, nitrogen, phosphorus, potassium and also the C/N ratio. Moisture content varied from 94 to 95 per cent, variation in carbon and nitrogen content was from 22.9 to 39.5 per cent and 2 to 4 per cent respectively among the locations studied. The C/N ratio ranged from 9 to 14. Crude protein content of Azolla registered values between 16 and 22 per cent. The content of phosphorus ranged between 0.20 and 0.23 per cent whereas that of potassium was in the range of 1.2 to 1.5 per cent. Correlation worked out between soil parameters and composition of Azolla disclosed a significant positive effect of soil P on the nitrogen, crude protein and phosphorus content of Azolla. However, soil P was negatively correlated with the C/N ratio of Azolla.
Nitrogenase enzyme activity quantified was profoundly affected by the locations. The amount of ethylene produced extended from 192 to 236 nmole ethylene g-1 h-1. It showed a significant negative correlation with soil pH and EC.
The study has given valuable information on the influence of soil and flood water quality parameters and locations on Azolla which could be used for further investigations on its nutrient dynamics.