Genetic diversity analysis of wild yams of Western Ghats
By: Irfa Anwar.
Contributor(s): Sheela M N (Guide).
Material type:
BookPublisher: Vellayani Department of Plant Biotechnology, College of Agriculture 2016Description: 63 pages.Subject(s): Plant BiotechnologyDDC classification: 660.6 Online resources: Click here to access online Dissertation note: MSc Summary: Wild Yams belonging to the family Dioscoreaceae makes significant contribution to the diets of the tribal community in India. They also have immense medicinal properties due to the presence of secondary metabolites especially diosgenin and hence is of great interest to the pharmaceutical industry. It serves as a precursor for the production of corticosteroids, sexual hormones, oral contraceptives and possesses cancer chemotherapeutic activity. In the present study conducted at ICAR-CTCRI, Thiruvanathapuram, morphological, biochemical and molecular variation among the major wild yams were studied to establish their genetic relationship. Morphological characterization of 25 accessions of wild yams (Dioscorea spp.) was done based on 21 IPGRI descriptors. Tuber weight/plant ranged from 40g (D. belophylla) to 5.66kg (D. floribunda) while tuber flesh color of the accessions varied from white to red purple. The biochemical studies on starch, sugar, crude protein, crude fat and crude fiber was done keeping in mind the nutritive value of the wild yam tubers used by the tribal community. Among the accessions, D. belophylla recorded the highest starch content on D.W basis (98%) while D. tomentosa the lowest (25%). Crude protein content was highest in D. floribunda with 14% followed by D. hispida (12.46%) and lowest value was recorded in D. wallichi (4.81%). From the genetic parameters estimated the fibre and sugar showed negative correlation to drymatter whereas sugar, protein and fibre recorded negative correlations to starch. The highest heritability was observed for crude protein ( 0.998). The wild yams were characterized at molecular level using 15 ISSR primers and 9 SSR primers, of which, UBC 808 and Dab2D06 showed high polymorphism for all the 18 species of wild yams studied. Principal component analysis was also performed to study the variation. The distribution of accessions in the scatter plot revealed high divergence of D. bulbiferra and D. floribunda. The cultivated greater yam (D. alata) was found to closely related to wild yam (D. opposittifolia). Hence the present study could identify the genetic relationship among wild yams and their nutritional quality.
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Theses
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KAU Central Library, Thrissur Theses | Reference Book | 660.6 IRF/GE (Browse shelf) | Not For Loan | 173914 |
MSc
Wild Yams belonging to the family Dioscoreaceae makes significant contribution to the diets of the tribal community in India. They also have immense medicinal properties due to the presence of secondary metabolites especially diosgenin and hence is of great interest to the pharmaceutical industry. It serves as a precursor for the production of corticosteroids, sexual hormones, oral contraceptives and possesses cancer chemotherapeutic activity. In the present study conducted at ICAR-CTCRI, Thiruvanathapuram, morphological, biochemical and molecular variation among the major wild yams were studied to establish their genetic relationship. Morphological characterization of 25 accessions of wild yams (Dioscorea spp.) was done based on 21 IPGRI descriptors. Tuber weight/plant ranged from 40g (D. belophylla) to 5.66kg (D. floribunda) while tuber flesh color of the accessions varied from white to red purple. The biochemical studies on starch, sugar, crude protein, crude fat and crude fiber was done keeping in mind the nutritive value of the wild yam tubers used by the tribal community. Among the accessions, D. belophylla recorded the highest starch content on D.W basis (98%) while D. tomentosa the lowest (25%). Crude protein content was highest in D. floribunda with 14% followed by D. hispida (12.46%) and lowest value was recorded in D. wallichi (4.81%). From the genetic parameters estimated the fibre and sugar showed negative correlation to drymatter whereas sugar, protein and fibre recorded negative correlations to starch. The highest heritability was observed for crude protein ( 0.998). The wild yams were characterized at molecular level using 15 ISSR primers and 9 SSR primers, of which, UBC 808 and Dab2D06 showed high polymorphism for all the 18 species of wild yams studied. Principal component analysis was also performed to study the variation. The distribution of accessions in the scatter plot revealed high divergence of D. bulbiferra and D. floribunda. The cultivated greater yam (D. alata) was found to closely related to wild yam (D. opposittifolia). Hence the present study could identify the genetic relationship among wild yams and their nutritional quality.
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