Molecular Characterization of sweet potato feathery mottle virus
By: Ashna, N K.
Contributor(s): MakeshKumar, T (Guide).
Material type:
BookPublisher: vellayani Department of Plant Biotechnology, College of Agriculture 2017Description: 64p.Subject(s): Biotechnology | Plant BiotechnologyDDC classification: 660.6 Online resources: Click here to access online Dissertation note: BSc-MSc (Integrated) Abstract: A study on “Molecular characterization of Sweet potato feathery mottle virus”
was conducted at the ICAR- Central Tuber Crop Research Institute, Sreekariyam,
Thiruvananthapuram during 2016-2017.
During the study 45 accessions of sweet potato from different fields of ICAR-
CTCRI were selected and different symptoms of Sweet potato feathery mottle virus
infection were recorded. The study reveals that the rate of infection is still prevailing
in Kerala having high symptom severity due to combination with other potyviruses
and increased aggressiveness as compared to earlier years because of the presence to
different strains.
Total RNA was isolated from all samples and used for RT-PCR analysis by
degenerate primers for potyviruses and specific primers for SPFMV to differentially
identify the presence of SPFMV. From the 45, five were confirmed as SPFMV
positive. Among this amplified products obtained from sample SH710 was
sequenced. From the 8 clones sequenced 3 different isolates of SPFMV is obtained,
Sweet potato feathery mottle virus isolate SPFMV-O-Arg polyprotein gene (NCBI
ID: KF386013.1) from Argentina, Sweet potato feathery mottle virus isolate CW137
(NCBI ID: KP115608.1) from Korean and Sweet potato feathery mottle virus isolate
Shanxi2 coat protein gene (NCBI ID: HQ844123.1) from China, these are further
belongs to broad class of Ordinary and Russet crack and East African. It further
showed the possibility of presence of RC-strain O-strain and EA-strain in Kerala.
To identify the phylogenetic relationship of the sequenced samples with that
of available accessions, dendrograms were made using MEGA 7.0.26 software and
the tree showed that sequences has variability eventhough lies within the group. By
restriction analysis and sequence analysis, variability was recorded within SPFMV in
Trivandrum.
| Item type | Current location | Collection | Call number | Status | Date due | Barcode |
|---|---|---|---|---|---|---|
Theses
|
KAU Central Library, Thrissur Theses | Reference Book | 660.6 ASH/MO (Browse shelf) | Not For Loan | 174180 |
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BSc-MSc (Integrated)
A study on “Molecular characterization of Sweet potato feathery mottle virus”
was conducted at the ICAR- Central Tuber Crop Research Institute, Sreekariyam,
Thiruvananthapuram during 2016-2017.
During the study 45 accessions of sweet potato from different fields of ICAR-
CTCRI were selected and different symptoms of Sweet potato feathery mottle virus
infection were recorded. The study reveals that the rate of infection is still prevailing
in Kerala having high symptom severity due to combination with other potyviruses
and increased aggressiveness as compared to earlier years because of the presence to
different strains.
Total RNA was isolated from all samples and used for RT-PCR analysis by
degenerate primers for potyviruses and specific primers for SPFMV to differentially
identify the presence of SPFMV. From the 45, five were confirmed as SPFMV
positive. Among this amplified products obtained from sample SH710 was
sequenced. From the 8 clones sequenced 3 different isolates of SPFMV is obtained,
Sweet potato feathery mottle virus isolate SPFMV-O-Arg polyprotein gene (NCBI
ID: KF386013.1) from Argentina, Sweet potato feathery mottle virus isolate CW137
(NCBI ID: KP115608.1) from Korean and Sweet potato feathery mottle virus isolate
Shanxi2 coat protein gene (NCBI ID: HQ844123.1) from China, these are further
belongs to broad class of Ordinary and Russet crack and East African. It further
showed the possibility of presence of RC-strain O-strain and EA-strain in Kerala.
To identify the phylogenetic relationship of the sequenced samples with that
of available accessions, dendrograms were made using MEGA 7.0.26 software and
the tree showed that sequences has variability eventhough lies within the group. By
restriction analysis and sequence analysis, variability was recorded within SPFMV in
Trivandrum.
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