Molecular characterization of rice genotypes having Variability in heat tolerance
By: Neethu V Mohan.
Contributor(s): Beena, R (Guide).
Material type:
BookPublisher: Vellayani Department of Plant Biotechnology, College of Agriculture 2017Description: 50p.Subject(s): BIotechnology | Plant biotechnologyDDC classification: 660.6 Online resources: Click here to access online Dissertation note: BSc. - MSc.(Integrated) Abstract: The study entitled “Molecular characterization of rice genotypes having
variability in heat tolerance” was conducted at the Department of Plant
Physiology, College of Agriculture, Vellayani, Thiruvananthapuram.
Rice (O. sativa) is very sensitive to high temperature especially at the
reproductive and grain filling stage which leads to higher spikelet sterility and
ultimately yield losses. The present investigation was, therefore, carried out with
the objective to study the variation in rice genotypes for heat tolerance using
microsatellite markers. In the present study, a set of 50 SSR primers were
employed to assess the genetic diversity among the 10 genotypes. Out of 50
markers, 11 markers showed polymorphism, the marker RM6100 was found as a
functional marker associated with heat tolerance in rice, and is functional for
further crop breeding programmes.
A dendrogram was generated with the aim of analyzing the relationships
between the 10 genotypes tested. The genetic similarity index ranged from 0.1 to
0.833. The lowest value 0.1was obtained between PTB7 and CR Dhan202 while
highest similarity value (0.833) calculated was between the PTB7 and CR
Dhan204 genotypes.
However, the genetic diversity analysis with SSR markers will contribute
to maximize the selection of diverse parents in the future rice breeding program or
development of heat tolerant cultivars.
Keywords: O. sativa, SSR markers, high temperature, heat tolerance,
spikelet fertility, genetic diversity
| Item type | Current location | Collection | Call number | Status | Date due | Barcode |
|---|---|---|---|---|---|---|
Theses
|
KAU Central Library, Thrissur Theses | Reference Book | 660.6 NEE/MO (Browse shelf) | Not For Loan | 174238 |
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BSc. - MSc.(Integrated)
The study entitled “Molecular characterization of rice genotypes having
variability in heat tolerance” was conducted at the Department of Plant
Physiology, College of Agriculture, Vellayani, Thiruvananthapuram.
Rice (O. sativa) is very sensitive to high temperature especially at the
reproductive and grain filling stage which leads to higher spikelet sterility and
ultimately yield losses. The present investigation was, therefore, carried out with
the objective to study the variation in rice genotypes for heat tolerance using
microsatellite markers. In the present study, a set of 50 SSR primers were
employed to assess the genetic diversity among the 10 genotypes. Out of 50
markers, 11 markers showed polymorphism, the marker RM6100 was found as a
functional marker associated with heat tolerance in rice, and is functional for
further crop breeding programmes.
A dendrogram was generated with the aim of analyzing the relationships
between the 10 genotypes tested. The genetic similarity index ranged from 0.1 to
0.833. The lowest value 0.1was obtained between PTB7 and CR Dhan202 while
highest similarity value (0.833) calculated was between the PTB7 and CR
Dhan204 genotypes.
However, the genetic diversity analysis with SSR markers will contribute
to maximize the selection of diverse parents in the future rice breeding program or
development of heat tolerant cultivars.
Keywords: O. sativa, SSR markers, high temperature, heat tolerance,
spikelet fertility, genetic diversity
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