Scientific validation of antiinflammatory, antinociceptive and antioxidant potential of malavirinji (Actinodaphne bourdillonii Gamble)
By: Adarsh Prathap.
Contributor(s): Suja, S R (Guide).
Material type:
BookPublisher: Vellayani Department of Plant Biotechnology, College of Agriculture 2017Description: 71p.Subject(s): Plant BiotechnologyDDC classification: 660.6 Online resources: Click here to access online Dissertation note: BSc-MSc (Integrated) Abstract: The study entitled “Scientific validation of antiinflammatory, antinociceptive and antioxidant potential of malavirinji (Actinodaphne bourdillonii Gamble)” was conducted at the Ethnomedicine and Ethnopharmacology Division of Jawaharlal Nehru Tropical Botanical Garden (JNTBGRI), Palode, Thiruvananthapuram, during the year 2016 to 2017. Objective of the study was to scientifically evaluate the antiinflammatory, antinociceptive, antioxidant potential of leaves of an ethnomedicinal plant Actinodaphne bourdillonii Gamble.
Phytochemical examination revealed the presence of various phytoconstituents like alkaloids, flavonoids, saponins, alkaloids, carbohydrates and phenols.
In in vitro antioxidant method the ethanolic extracts of leaf showed higher free radical scavenging activity compared to standards with IC50 of DPPH, NO Scavenging Activity, Hydroxyl Free Radical Scavenging Activity, Superoxide radical scavenging activity, Total antioxidant activity and Ferric reducing antioxidant potential (FRAP) assay.
Toxicity of the ethanolic extract of leaves of A. bourdillonii were tested by acute toxicity study in mice with four doses 25, 100, 400, 1600 mg/kg body weight. And the mice were cage side observed for fourteen days and no toxic effect were seen in the tested animals.
In the detailed in vitro pharmacological studies for antiinflammatory and antinociception were conducted. Antiinflammatory activity was determined by Carrageenan (Acute inflammation) and formalin (sub chronic inflammation) induced paw oedema on hind limb in rats with three different doses 50, 150 and 450 mg/kg. At the dose of EAB 450mg/kg give 83.63% of inhibition in carrageenan induced paw oedema and in the formalin induced paw oedema (sub-acute) study a dose of EAB 150mg/kg gives maximum inhibition of 70.23% and 78.36% of inhibition in the first and seventh day respectively. Antinociception activity was determined by Eddy Hot Plate method and Acetic acid writhing method in mice with three different doses 50, 150 and 450 mg/kg. At the dose of EAB 450mg/kg gives maximum inhibition 87.76% of inhibition in Eddy’s hot plate method and in the acetic acid induce writhing study a dose of EAB 450mg/kg gives maximum inhibition of 71.55% of inhibition.
| Item type | Current location | Collection | Call number | Status | Date due | Barcode |
|---|---|---|---|---|---|---|
Theses
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KAU Central Library, Thrissur Theses | Reference Book | 660.6 ADA/SC (Browse shelf) | Not For Loan | 174343 |
BSc-MSc (Integrated)
The study entitled “Scientific validation of antiinflammatory, antinociceptive and antioxidant potential of malavirinji (Actinodaphne bourdillonii Gamble)” was conducted at the Ethnomedicine and Ethnopharmacology Division of Jawaharlal Nehru Tropical Botanical Garden (JNTBGRI), Palode, Thiruvananthapuram, during the year 2016 to 2017. Objective of the study was to scientifically evaluate the antiinflammatory, antinociceptive, antioxidant potential of leaves of an ethnomedicinal plant Actinodaphne bourdillonii Gamble.
Phytochemical examination revealed the presence of various phytoconstituents like alkaloids, flavonoids, saponins, alkaloids, carbohydrates and phenols.
In in vitro antioxidant method the ethanolic extracts of leaf showed higher free radical scavenging activity compared to standards with IC50 of DPPH, NO Scavenging Activity, Hydroxyl Free Radical Scavenging Activity, Superoxide radical scavenging activity, Total antioxidant activity and Ferric reducing antioxidant potential (FRAP) assay.
Toxicity of the ethanolic extract of leaves of A. bourdillonii were tested by acute toxicity study in mice with four doses 25, 100, 400, 1600 mg/kg body weight. And the mice were cage side observed for fourteen days and no toxic effect were seen in the tested animals.
In the detailed in vitro pharmacological studies for antiinflammatory and antinociception were conducted. Antiinflammatory activity was determined by Carrageenan (Acute inflammation) and formalin (sub chronic inflammation) induced paw oedema on hind limb in rats with three different doses 50, 150 and 450 mg/kg. At the dose of EAB 450mg/kg give 83.63% of inhibition in carrageenan induced paw oedema and in the formalin induced paw oedema (sub-acute) study a dose of EAB 150mg/kg gives maximum inhibition of 70.23% and 78.36% of inhibition in the first and seventh day respectively. Antinociception activity was determined by Eddy Hot Plate method and Acetic acid writhing method in mice with three different doses 50, 150 and 450 mg/kg. At the dose of EAB 450mg/kg gives maximum inhibition 87.76% of inhibition in Eddy’s hot plate method and in the acetic acid induce writhing study a dose of EAB 450mg/kg gives maximum inhibition of 71.55% of inhibition.
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