Heat shock protein 70 expression in different vital organs of heat stressed Malabari goats
By: Afsal, A.
Contributor(s): Sejian, V (Guide).
Material type:
BookPublisher: Vellanikkara Academy of Climate Change Education and Research 2018Description: 72p.Subject(s): Climate Change | Heat stress | HSP70 | Malabari Goat | AdaptationDDC classification: 551.6 Online resources: Click here to access online Dissertation note: BSc-MSc (Integrated) Abstract: The study was conducted to establish the impact of heat stress on the expression pattern of heat shock protein 70 (HSP70) gene in different organs of Malabari goats. The study was conducted for a period of 45 days in twelve 10 months to one year old Malabari goats randomly allocated into two groups: MC (n=6; Malabari control) and MHS (n=6; Malabari Heat stress). Goats were stall-fed with a diet composed of 60% roughage and 40% concentrate. The MC goats were placed in the shaded pens while MHS goats exposed to heat stress in outside environment between 10.00 h to 16.00 h. At the end of study period, all 12 animals were slaughtered and their adrenal, thyroid, liver, uterus and mesenteric lymph node (MLN) tissues were collected for HSP70 gene expression. The temperature-humidity index (THI) inside shed (74.9) proved that the animals were not stressed while in the outside environment (86.5) the animals were extremely distressed. Heat stress significantly increased the HSP70 expression pattern in adrenal while significantly lowered the HSP70 expression pattern in thyroid and MLN. However, heat stress did not influence the expression pattern of HSP70 gene in both liver and uterus. Further, a strong positive correlation was established between temperature-humidity-indexes (THI) and liver and uterus while negative correlation with thyroid and LN. Thus, it can be concluded that the expression pattern of HSP70 gene was tissue and function specific and it was programmed according to the requirement of the heat stressed animals of Malabari goats.
| Item type | Current location | Collection | Call number | Status | Date due | Barcode |
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Theses
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KAU Central Library, Thrissur Theses | Reference Book | 551.6 AFS/HE (Browse shelf) | Not For Loan | 174564 |
BSc-MSc (Integrated)
The study was conducted to establish the impact of heat stress on the expression pattern of heat shock protein 70 (HSP70) gene in different organs of Malabari goats. The study was conducted for a period of 45 days in twelve 10 months to one year old Malabari goats randomly allocated into two groups: MC (n=6; Malabari control) and MHS (n=6; Malabari Heat stress). Goats were stall-fed with a diet composed of 60% roughage and 40% concentrate. The MC goats were placed in the shaded pens while MHS goats exposed to heat stress in outside environment between 10.00 h to 16.00 h. At the end of study period, all 12 animals were slaughtered and their adrenal, thyroid, liver, uterus and mesenteric lymph node (MLN) tissues were collected for HSP70 gene expression. The temperature-humidity index (THI) inside shed (74.9) proved that the animals were not stressed while in the outside environment (86.5) the animals were extremely distressed. Heat stress significantly increased the HSP70 expression pattern in adrenal while significantly lowered the HSP70 expression pattern in thyroid and MLN. However, heat stress did not influence the expression pattern of HSP70 gene in both liver and uterus. Further, a strong positive correlation was established between temperature-humidity-indexes (THI) and liver and uterus while negative correlation with thyroid and LN. Thus, it can be concluded that the expression pattern of HSP70 gene was tissue and function specific and it was programmed according to the requirement of the heat stressed animals of Malabari goats.
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