Physiological and molecular studies on cyanogenic potential in cassava (Manihot esculenta Crantz) in response to nitrogen nutrition, water stress and shade
By: Achuth P. Jayaraj.
Contributor(s): Saravanan Raju (Guide).
Material type:
BookPublisher: Vellayani Department of Plant Biotechnology College of Agriculture 2018Description: 72p.Subject(s): BiotechnologyDDC classification: 660.6 Online resources: Click here to access online Dissertation note: BSc-MSc (Integrated) Abstract: The study entitled “Physiological and molecular studies on cyanogenic potential in cassava (Manihot esculenta Crantz) in response to nitrogen nutrition, water stress and shade” was carried out at the Division of Crop utilization, ICAR- Central Tuber Crops Research Institute, Sreekariyam, Thiruvananthapuram during the year 2017-2018. To study the physiology and molecular aspects of biosynthesis of cyanogenic glycosides in cassava in response to plant nitrogen status, light intensity and water stress and to compare detection methods such as Near-infra red (NIR) spectroscopy and High Performance Thin Layer Chromatography (HPTLC) for cyanogenic glycoside estimation. Cassava (Manihot esculenta Crantz) contains cyanogenic glycosides like linamarin, acetone cyanohydrin, and hydrocyanic acid. The concentration of cyanogens in roots and leaves differ in the same plant and is known to be more abundant in the leaves than the stem and roots. The synthesis and regulation of bioactive natural products are influenced by changes in the nutritional availability of both micro and macro. It is important to be aware of and understand the consequences of such changes so that appropriate measures can be taken to either reduce risks or capitalize on benefits. Here in this study not much difference was observed between plants grown in shade irrigated and open irrigated in level of CNglc content. Plants grown in shade water stress have CNglc amount at par with plants grown in open water stress. Increased nitrogen supply stimulates plant growth and productivity as well as photosynthetic capacity of leaves through increased amounts of stromal and thylakoid proteins in leaves. Here CNglc content increase according to nitrogen nutrition doesn’t seems to follow a uniform pattern as in case of Sree Vijaya CNglc content remains unchanged or a small decrease is seen. Also here although an increase is seen in CNglc content from 0.5x to 1x strength, increase is not seen in 2x strength solution. Detection of CNglc in plant samples are important for not only quality control but for many other aspects of biochemical research. Various techniques are used for estimating CNglc in cassava samples and the enzymatic and spectro-photometric techniques are routinely used. From this study NIR data and HPTLC data obtained from this investigation can be used for developing methods for quantifying the CNglc content directly in plant samples in the future without cumbersome sample preparation techniques.
| Item type | Current location | Collection | Call number | Status | Date due | Barcode |
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Theses
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KAU Central Library, Thrissur Theses | Reference Book | 660.6 ACH/PH (Browse shelf) | Not For Loan | 174550 |
BSc-MSc (Integrated)
The study entitled “Physiological and molecular studies on cyanogenic potential in cassava (Manihot esculenta Crantz) in response to nitrogen nutrition, water stress and shade” was carried out at the Division of Crop utilization, ICAR- Central Tuber Crops Research Institute, Sreekariyam, Thiruvananthapuram during the year 2017-2018. To study the physiology and molecular aspects of biosynthesis of cyanogenic glycosides in cassava in response to plant nitrogen status, light intensity and water stress and to compare detection methods such as Near-infra red (NIR) spectroscopy and High Performance Thin Layer Chromatography (HPTLC) for cyanogenic glycoside estimation. Cassava (Manihot esculenta Crantz) contains cyanogenic glycosides like linamarin, acetone cyanohydrin, and hydrocyanic acid. The concentration of cyanogens in roots and leaves differ in the same plant and is known to be more abundant in the leaves than the stem and roots. The synthesis and regulation of bioactive natural products are influenced by changes in the nutritional availability of both micro and macro. It is important to be aware of and understand the consequences of such changes so that appropriate measures can be taken to either reduce risks or capitalize on benefits. Here in this study not much difference was observed between plants grown in shade irrigated and open irrigated in level of CNglc content. Plants grown in shade water stress have CNglc amount at par with plants grown in open water stress. Increased nitrogen supply stimulates plant growth and productivity as well as photosynthetic capacity of leaves through increased amounts of stromal and thylakoid proteins in leaves. Here CNglc content increase according to nitrogen nutrition doesn’t seems to follow a uniform pattern as in case of Sree Vijaya CNglc content remains unchanged or a small decrease is seen. Also here although an increase is seen in CNglc content from 0.5x to 1x strength, increase is not seen in 2x strength solution. Detection of CNglc in plant samples are important for not only quality control but for many other aspects of biochemical research. Various techniques are used for estimating CNglc in cassava samples and the enzymatic and spectro-photometric techniques are routinely used. From this study NIR data and HPTLC data obtained from this investigation can be used for developing methods for quantifying the CNglc content directly in plant samples in the future without cumbersome sample preparation techniques.
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