PhD

Information on the specific role of onzymes
in controlling
the various
biochemical events leading to formation of an egg in the avian oviduct is scanty.
Hence, it was considered worth while to investigate the
onzyme pattern in the
plasma and in the reproductive organs in white Leghorn and white Plymouth Rock
breeds of fowls, and to studyt the tissue localization of acid and alkaline
phosphatases by histochemical techniques. The influences of various oxogenous sex
hormones on the development and onzyne pattern of the female reproductive organs
was also studied.
The blood plasma and tissue homogenatee of the reproductive organs were
assayed for alkaline
phosphatase, acid
phosphatase, glutamate
oxaloacetate
transaainace , acid phosphatase, glutante pyruvate transaminases and glucose-6-
phosphatase.
Many of the enzymes studied were present in the plasma and tissue
homogenates without any significant breed difference. Acid
phosphatase activity
could not be detected in he blood [plasma of one-to-two-months-old white Leghorn
and White Plymouth rock birds. In the white plymouth rock chicken,ma glutanate
pyruvate transaminase activity also could not be detected. Alkaline
activity was higher in the ovary of White Leghorn breed of fowls.
phosphatase
In the two-to-three-month-old pullets, White Plymouth rock bird showed
higher concentration of glucose-6-phosphatase in their oviducts.
Five-to six-month-old folwle of both the breed did not show any plasma acid
phosphatase activity. But in the infundibulue, activities of both the transminaces
were higher in the White Leghorn fowle. Glutamate pyruvate tranminance activity
in white Plyouth rock oviducvt was conficed to the megaum and isthmue.
While there was not significant difference in the morophological development
of the oviduet in immature chicken under the influences of different combination of
stilbestrol diprepienate and
testoateronar propionate or stilbestrol dipropionate
and progeetrone compared to chickens on stil bestrol dipropionate alone the White
Leghorn chickens receiving stilbostrol dipropinate and testoaterins propicnate
showed higher ovariam levels of acid phosphatase and glutamate
transminase.
pyruvate
Oviducal contents of acid phosphatase, glutamate oxalocetate and
glutamate pyruvate transminases were also high.
In the white Plymouth rock
ehicken, on the other hand, lower concentration of plasma
glutnate pyruvate
transmainase and lower levels of oviducal alkaline phosphatase, acid phophatase
and glutanate oxalocetate transminace were evident.
In between the two
experimental groups, the white Plymouth rock chickens had higher content of acid
phosphatase in the iethmus and glucose-6-phosphatase in the varina. But,
among the control groups higher contents of plasma
glutamae pyruvate
transaminase and oviducak alkaline phosphatase were seen in he whit plymouth
rock chicks.
Stilbestrol dipropionate and progreaterone administation resulted in higher
oviducal concentraion of glutamate oxalocacetate tamsmionase in white plymouth
rock chicks. Glutamate pyruvate tranmnase activity could tissues studied. In white
Leghorn experimental chicks the ovarian acid phosphatase content was less than
their corresponding control chicks.
Histochemical localisation of alkline phosphatase was seen in all the region of
the female reproductive organs in both the breeds. Ovarian localization of the
enzyme was in the follicular optithelium and strima with strong reaction for the
enzyme in many of the blood veacole. The reaction was of stronger intensity in
magmm and gvagina coupared to other regionsof the oviduct. Localization of acid
phosphatase was similar to that of alkaline phosphatase.
The histochemical localization of acid and alkaline
phosphatase in the
various regions of the female reproductive organs developed under the influence of
different sex hormones were similar to those seen in adult fowls.
However, the intensity of the staining reaction for the
ensyme in the reproductive
organs of chicks developed under the influence of stilbestrol dipropionate was more
compared to that of the other two experimental groups.