MVSc

The objective of the study was to find out the optimum number of collections per day which can be taken from adult cross – bred bucks without adversely affecting their libido and semen quality. Ejaculation frequencies of once, twice and thrice daily for a continuous period of three months were adopted for the study. Epididymal sperm reserves and Daily Sperm Production were estimated to assess the sperm reserves of bucks. While colour, density, mass activity, concentration and MBR time were found to be influenced by ejaculation frequencies, volume, pH, percentage of dead sperms, percentage of abnormal sperms and sperm vialbility were unaffected. Frequency of ejaculation was not found to affect the libido of bucks. Bucks within group were found to influence all seminal attributes except mass activity, percentage of dead sperms and MBR time. Similarly all the seminal attributes with the exception of proximal and distal protoplasmic droplets were influenced by months within groups. A positive correlation between sperm viability at 46.50C for 30 minutes and preservation at 6 to 80C for 96 hrs.was observed. While in group II bucks only density, mass activity, pH, concentration and MBR time showed significant difference between ejaculates, in group 111, all seminal attributes except percentage of dead sperms were observed to be significantly different between ejaculates.
The Daily Sperm Production per buck (X 109), per gram of testis (X 106), Daily Sperm Output (X 109) and dpididymal Sprem reserves (X 109) were respectively 3.7949 + 0.2032, 22.1974 + 0.3775, 1.2680 and 25.72 + 1.95. The average transit time of spermatozoa through epididymis was found to be 6.78 days. Increasing the frequency of collection from once daily to twice or thrice daily resulted in a significant drop in spermatozoan concentration, thus affecting seminal attributes such as colour, density, mass activity and MBR time. The very fact that other important seminal attributes such as volume, pH, motility, percentage of abnormal sperms, percentage of dead sperms, percentage of proximal and distal protoplasmic droplets and spermatozoan viability were unaffected, clearly points out that there is no deterioration of semen quality with increasing collection frequency. Similarly there was no deterioration in the sex libido and fertility of bucks even with three collections daily. However, there dose not seem to be any definite advantage in increasing collection frequency from 2 to 3 times daily, as the total harvest of sperms from group 11 and group 111 were almost same. But increasing the frequency of collection from one to two times daily has definite advantage, as it yields more spermatozoa for artificial insemination and hence is recommended for adoption.