Standardisation Of in vitro Propagation Technique In Clove Lyzygium aromaticum(L.)
By: Shammy Mathew.
Contributor(s): Rajendran PC (Guide).
Material type:
BookPublisher: Vellanikkara Department of Plantation Crops and Spices, College of Horticulture 1994DDC classification: 633.8 Online resources: Click here to access online | Click here to access online Dissertation note: MSc Abstract: A study was taken up in the Department of Plantation Crops and Spices, College of Horticulture, Vellanikkara, during 1992 to 1994 to standardize the in vitro propagation technique in clove syzygium aromaticum (L.). Mature field grown trees and seedlings of clove were used as sources of explants in the present study. Different routes like enhanced release of axillary buds, and callus mediated organogenesis/embryogenesis were attempted for the crop.
Culture establishment of mature field grown clove explants faced two serious problems viz. fungal contamination caused by Alternaria sp. And polyphenol interference. Prophylactic spraying given to the mother plants with aureofunginsol gave 44 per cent reduction in the contamination rate. Polyphenol interference was completely controlled with wax sealing treatment given to the explants, or by supplementing the culture medium with polyvinyl pyrrolidone or activated charcoal. Multiple shoots were induced from nodal explants cultured in WPM supplemented with BAP (3.0 mg1-1) and kinetin (1.0 mg1-1).
Seedling explants of clove showed very good culture establishment. Microbial contamination and polyphenol interference were observed only to a very limited extent. Multiple shoots were induced from nodal explants cultured in half-MS and WPM supplemented with lower levels and combinations of growth regulators like BAP, kinetin, IAA and NAA. Additives like CW induced vigorous shoots whereas adenine sulphate and phloroglucinol did not give any favourable response for multiple shoot induction. Incorporation of activated charcoal and very low level of BAP (0.2 mg1 -1) favoured elongation of shoots and leaf production. Shoot explants showed rooting in WPM containing IBA and NAA each at 2.0 mg1-1.
Callus could be induced from leaf and internodal segments of clove seedling cultured in half-MS medium supplemented with 2,4-D, NAA or BAP + IAA. Addition of casein hydrolysate favoured callus induction and proliferation. Calli induced with 0.5 mg1-1 of 2,4-D produced shining globular structures resembling proembryoids. Calli obtained were failed to induce organogenesis with the treatments tried.
Due to low meristematic activity clove plants are difficult to propagate vegetatively. Moreover clove is reported to be the slowest growing tree. The same property is being reflected in the in vitro cultures as well, hence much more concerted efforts are required to develop a viable protocol for the micropropagation of clove syzygium aromaticum (L.)
| Item type | Current location | Call number | Status | Date due | Barcode |
|---|---|---|---|---|---|
Theses
|
KAU Central Library, Thrissur Theses | 633.8 SHA/ST (Browse shelf) | Available | 170537 |
MSc
A study was taken up in the Department of Plantation Crops and Spices, College of Horticulture, Vellanikkara, during 1992 to 1994 to standardize the in vitro propagation technique in clove syzygium aromaticum (L.). Mature field grown trees and seedlings of clove were used as sources of explants in the present study. Different routes like enhanced release of axillary buds, and callus mediated organogenesis/embryogenesis were attempted for the crop.
Culture establishment of mature field grown clove explants faced two serious problems viz. fungal contamination caused by Alternaria sp. And polyphenol interference. Prophylactic spraying given to the mother plants with aureofunginsol gave 44 per cent reduction in the contamination rate. Polyphenol interference was completely controlled with wax sealing treatment given to the explants, or by supplementing the culture medium with polyvinyl pyrrolidone or activated charcoal. Multiple shoots were induced from nodal explants cultured in WPM supplemented with BAP (3.0 mg1-1) and kinetin (1.0 mg1-1).
Seedling explants of clove showed very good culture establishment. Microbial contamination and polyphenol interference were observed only to a very limited extent. Multiple shoots were induced from nodal explants cultured in half-MS and WPM supplemented with lower levels and combinations of growth regulators like BAP, kinetin, IAA and NAA. Additives like CW induced vigorous shoots whereas adenine sulphate and phloroglucinol did not give any favourable response for multiple shoot induction. Incorporation of activated charcoal and very low level of BAP (0.2 mg1 -1) favoured elongation of shoots and leaf production. Shoot explants showed rooting in WPM containing IBA and NAA each at 2.0 mg1-1.
Callus could be induced from leaf and internodal segments of clove seedling cultured in half-MS medium supplemented with 2,4-D, NAA or BAP + IAA. Addition of casein hydrolysate favoured callus induction and proliferation. Calli induced with 0.5 mg1-1 of 2,4-D produced shining globular structures resembling proembryoids. Calli obtained were failed to induce organogenesis with the treatments tried.
Due to low meristematic activity clove plants are difficult to propagate vegetatively. Moreover clove is reported to be the slowest growing tree. The same property is being reflected in the in vitro cultures as well, hence much more concerted efforts are required to develop a viable protocol for the micropropagation of clove syzygium aromaticum (L.)
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