Mutagenicity Of Gamma Rays And EMS On Winged Bean
By: Deepa TO.
Contributor(s): RadhaDevi Ds (Guide).
Material type:
BookPublisher: Vellayani Department of Plant breeding and Genetics, College of Agriculture 1995DDC classification: 630.28 Online resources: Click here to access online Dissertation note: MSc Abstract: A study on mutagenicity of gamma rays and EMS in the M2 generation of winged bean [Psophocarpus tetragonolobus (L.)] was carried out in non-replicated progeny row trial with seeds collected individually from M1 plants which were initially treated with four doses of gammarays (100, 200, 300 and 400 Gy units) and five doses of EMS (40, 80, 120, 160 and 200 mM). Observations on chlorophyll and viable mutations and other characters like height at 45 DAS and 75 DAS, days to flower. Pod length, pod number, seed number and 100 seed weight were recorded. The chlorophyll and viable mutations were quantitatively expressed by frequency and qualitatively by spectrum. The frequency distribution, range and mean performance of plants in each treatment were estimated.
Quantitative analysis of chlorophyll mutations showed that mutation frequencies recorded an increase at the lower doses and a decrease at he higher doses. The frequencies were maximum at the same dose for both the mutagens viz., at 200 Gy units of gamma rays and 160 mM of EMS. Among the two mutagens, high frequencies were noticed for gamma rays.
Quantitative analysis of chlorophyll mutations identified six types for both the mutagens viz., xantha, chlorina, viridis, maculata, viridalba and white streaked types. But none of the treatments produced six types together. Among the different types, chlorina appeared as the most frequent one. In case of EMS, the decrease in multiple mutation frequency with increase in dose was clearly observed. The segregation per cent of most of the chlorophyll mutants was in between 10 and 14.
Quantitative analysis of viable mutations revealed that maximum frequency on M2 plant basis was’nt at the higher doses for both the mutagens. Twenty one viable mutants with change in characters of leaf, stem, pod and seed were isolated from gamma ray treated population and twenty two from EMS treated population.
A negative shift in mean was noticed for all the characters except days to flower due to more negative variants than positive variants even in the lower doses. The mean performance of plants was found to be minimum for higher doses and maximum for control and lower doses.
Mutagenic effectiveness was found to be high in lower doses viz., 100 Gy units of gamma rays and 40 mM of EMS. The efficient doses of gamma rays identified on the basis of injury and lethality or sterility were 100 Gy units and 200 Gy units respectively. In EMS the efficiency on the basis of sterility, lethality and injury was maximum at 120, 160 and 80 mM respectively.
| Item type | Current location | Call number | Status | Date due | Barcode |
|---|---|---|---|---|---|
Theses
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KAU Central Library, Thrissur Theses | 630.28 DEE/MU (Browse shelf) | Available | 170619 |
MSc
A study on mutagenicity of gamma rays and EMS in the M2 generation of winged bean [Psophocarpus tetragonolobus (L.)] was carried out in non-replicated progeny row trial with seeds collected individually from M1 plants which were initially treated with four doses of gammarays (100, 200, 300 and 400 Gy units) and five doses of EMS (40, 80, 120, 160 and 200 mM). Observations on chlorophyll and viable mutations and other characters like height at 45 DAS and 75 DAS, days to flower. Pod length, pod number, seed number and 100 seed weight were recorded. The chlorophyll and viable mutations were quantitatively expressed by frequency and qualitatively by spectrum. The frequency distribution, range and mean performance of plants in each treatment were estimated.
Quantitative analysis of chlorophyll mutations showed that mutation frequencies recorded an increase at the lower doses and a decrease at he higher doses. The frequencies were maximum at the same dose for both the mutagens viz., at 200 Gy units of gamma rays and 160 mM of EMS. Among the two mutagens, high frequencies were noticed for gamma rays.
Quantitative analysis of chlorophyll mutations identified six types for both the mutagens viz., xantha, chlorina, viridis, maculata, viridalba and white streaked types. But none of the treatments produced six types together. Among the different types, chlorina appeared as the most frequent one. In case of EMS, the decrease in multiple mutation frequency with increase in dose was clearly observed. The segregation per cent of most of the chlorophyll mutants was in between 10 and 14.
Quantitative analysis of viable mutations revealed that maximum frequency on M2 plant basis was’nt at the higher doses for both the mutagens. Twenty one viable mutants with change in characters of leaf, stem, pod and seed were isolated from gamma ray treated population and twenty two from EMS treated population.
A negative shift in mean was noticed for all the characters except days to flower due to more negative variants than positive variants even in the lower doses. The mean performance of plants was found to be minimum for higher doses and maximum for control and lower doses.
Mutagenic effectiveness was found to be high in lower doses viz., 100 Gy units of gamma rays and 40 mM of EMS. The efficient doses of gamma rays identified on the basis of injury and lethality or sterility were 100 Gy units and 200 Gy units respectively. In EMS the efficiency on the basis of sterility, lethality and injury was maximum at 120, 160 and 80 mM respectively.
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