Indirect Organogenesis And Embryogenesis In Kaempferia Galanga L.
By: Mini Joseph.
Contributor(s): Lissamma Joseph (Guide).
Material type:
BookPublisher: Vellanikkara Department of Plantation Crops and Spices, College of Horticulture 1997DDC classification: 633.8 Online resources: Click here to access online Dissertation note: MSc Abstract: A study was taken up in the Department of Plantation Crops and Spices, College of Horticulture, Vellanikkara, during 1994-’96, to standardize the technique of indirect organogenesis and embryogenesis in Kaempferia galangal and to evaluate the field performance of the plantlets as compared with conventional propagules. Axenic cultures were raised from rhizome buds as explaint source for callus induction.
Pseudostem bits, leaf bits and pseudostem base were tried as explants for inducing calli. Profuse callusing could be induced from pseudostem bit explant in ½ MS medium supplemented with 2, 4-D and low concentrations of BA. The calli induced were creamy and friable with good morphogenic potential.
Shoot regeneration was obtained from the calli in ½ MS medium supplemented with BA 8.0 mg 1-1 with an average of 5.6 shoots per culture tube. Kinetin was also effective in inducing shoots from the calli and maximum number of shoots were produced at kinetin 6 mg 1-1 concentration. Only rhizogenesis was observed with combinations of 2, 4-D with lower levels of BA and with silver nitrate (5.0 and 10 mg 1-1) and activated charcoal (0.1%). Suspension cultures were not effective for inducing organogenesis/embryogenesis.
Compact, shiny, white embryogenic calli were induced in the peripheral region of cultures inoculated in ½ MS + KIN. Under dark incubation, numerous embryoids were induced in MS medium supplemented with NAA (3 mg1-1) and BA (1 mg 1-1). MS medium with sucrose at five per cent level was the best medium for maturation and germination of embryoid. All the stages of somatic embryoids were observed in the cultures.
The plantlets produced through indirect organogenesis and embryogenesis were hardened and successfully planted out. The tissue culture derived plants were compared with conventionally propagated plants in the field. The percentage of survival and leaf area per leaf of tissue culture derived plants were low compared to conventionally propagated plants. But the number of tillers produced per clump was very high in plants produced through indirect organogenesis and embryogenesis and the leaf orientation was erect.
The results of present study reveal that there is scope of successful indirect organogenesis and embryogenesis in kaempferia and there exists significant variation between tissue culture derived and conventionally propagated plants and there is scope of exploiting this variation to complement the limited natural variability in this crop.
| Item type | Current location | Call number | Status | Date due | Barcode |
|---|---|---|---|---|---|
Theses
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KAU Central Library, Thrissur Theses | 633.8 MIN/IN (Browse shelf) | Available | 170860 |
MSc
A study was taken up in the Department of Plantation Crops and Spices, College of Horticulture, Vellanikkara, during 1994-’96, to standardize the technique of indirect organogenesis and embryogenesis in Kaempferia galangal and to evaluate the field performance of the plantlets as compared with conventional propagules. Axenic cultures were raised from rhizome buds as explaint source for callus induction.
Pseudostem bits, leaf bits and pseudostem base were tried as explants for inducing calli. Profuse callusing could be induced from pseudostem bit explant in ½ MS medium supplemented with 2, 4-D and low concentrations of BA. The calli induced were creamy and friable with good morphogenic potential.
Shoot regeneration was obtained from the calli in ½ MS medium supplemented with BA 8.0 mg 1-1 with an average of 5.6 shoots per culture tube. Kinetin was also effective in inducing shoots from the calli and maximum number of shoots were produced at kinetin 6 mg 1-1 concentration. Only rhizogenesis was observed with combinations of 2, 4-D with lower levels of BA and with silver nitrate (5.0 and 10 mg 1-1) and activated charcoal (0.1%). Suspension cultures were not effective for inducing organogenesis/embryogenesis.
Compact, shiny, white embryogenic calli were induced in the peripheral region of cultures inoculated in ½ MS + KIN. Under dark incubation, numerous embryoids were induced in MS medium supplemented with NAA (3 mg1-1) and BA (1 mg 1-1). MS medium with sucrose at five per cent level was the best medium for maturation and germination of embryoid. All the stages of somatic embryoids were observed in the cultures.
The plantlets produced through indirect organogenesis and embryogenesis were hardened and successfully planted out. The tissue culture derived plants were compared with conventionally propagated plants in the field. The percentage of survival and leaf area per leaf of tissue culture derived plants were low compared to conventionally propagated plants. But the number of tillers produced per clump was very high in plants produced through indirect organogenesis and embryogenesis and the leaf orientation was erect.
The results of present study reveal that there is scope of successful indirect organogenesis and embryogenesis in kaempferia and there exists significant variation between tissue culture derived and conventionally propagated plants and there is scope of exploiting this variation to complement the limited natural variability in this crop.
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