Development of Inculant Cultures of Azospirillum for Rice (Oryza Sativa L.) in Kuttanad
By: Sasikumar S.
Contributor(s): Sivaprasad P (Guide).
Material type:
BookPublisher: Vellayani Department of Plant Pathology, College of Agriculture 1996DDC classification: 632.3 Online resources: Click here to access online Dissertation note: MSc Abstract: Azospirillum was isolated from the rice roots of twenty five different locations of Kuttanad, Kerala in order to develop acid and salt tolerant inoculant cultures suited for the locality. The isolates were identified as A lipoferum and A brasilense. All the isolates showed good salt tolerance up to 2% NaCl concentration and good acid tolerance up to pH 5.0 and some isolates tolerated up to pH 4.0. The nitrogen fixing capacity in vitro by the isolates ranged between 11.20 and 2.28 mg/g malate and 1AA production between 24 µg 1-1 and 55 µg 1-1. Four isolates of them were selected for pot culture trials based on efficiency of nitrogen fixation, 1AA production, root elongation and acid and salt tolerance. The inoculated plants showed increased height, number of productive tillers and beneficial influence on root parameters. All these reflected in the final grain yield and AZR 15 gave highest yield followed by AZR 37. A saving of 25% nitrogen can be made by inoculation with these cultures in rice.
The acridine orange curing of plasmids conducted to know the genetic basis of salt tolerance showed that the character is plasmid borne.
| Item type | Current location | Call number | Status | Date due | Barcode |
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Theses
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KAU Central Library, Thrissur Theses | 632.3 SAS/DE (Browse shelf) | Available | 170878 |
MSc
Azospirillum was isolated from the rice roots of twenty five different locations of Kuttanad, Kerala in order to develop acid and salt tolerant inoculant cultures suited for the locality. The isolates were identified as A lipoferum and A brasilense. All the isolates showed good salt tolerance up to 2% NaCl concentration and good acid tolerance up to pH 5.0 and some isolates tolerated up to pH 4.0. The nitrogen fixing capacity in vitro by the isolates ranged between 11.20 and 2.28 mg/g malate and 1AA production between 24 µg 1-1 and 55 µg 1-1. Four isolates of them were selected for pot culture trials based on efficiency of nitrogen fixation, 1AA production, root elongation and acid and salt tolerance. The inoculated plants showed increased height, number of productive tillers and beneficial influence on root parameters. All these reflected in the final grain yield and AZR 15 gave highest yield followed by AZR 37. A saving of 25% nitrogen can be made by inoculation with these cultures in rice.
The acridine orange curing of plasmids conducted to know the genetic basis of salt tolerance showed that the character is plasmid borne.
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