Induced Maturation of Penaeus Indicus Using Exogenous Hormone
By: Johnson D`cruz.
Contributor(s): Sebastian M J (Guide).
Material type:
BookPublisher: Panangad Department of Aquaculture, College of Fisheries 1995DDC classification: 639.2 Online resources: Click here to access online Dissertation note: MSc Abstract: The ovarian maturation and spawning of broodstock shrimp play a major role in the development of commercial level seed production of this valuable crustacean. The already proven technology of induced maturation of penaeids in capativity viz. Unilateral Eyestalk Ablation(UESA) has many negative effects which prompted scientists to explore alternative ways of induced maturation like hormone administration. The hormone progesterone alone and in combination with tocopherol were tested for their effectiveness in bringing about ovary maturation and spawning in pond reared penaeus indicus.
Progesterone and tocopherol dissolved in ethyl alcohol were injected at three test concentrations of 0.05 ug, 0.10ug and 0.20ug/g body weight . For the preparation of combination test solution the dissolved progesterone and tocopherol were mixed in the ratio of 7:3(V/V). Control shrimps were given alcohol treatment at respective concentrations.
The results of the experiment conducted for a period of after 45 days, revealed that the overall survival is not affected (89%) and that progesterone singly or in combination with tocopherol is capable of giving stimulation for ovarian development in P.Indicus Though there was not much observable difference in ovarian development by external viewing, both progesterone alone and in combination with tocopherol at higher dosages have yielded significant GSI difference and ovarian growth, compared with the ethanol treated controls, when the ovaries were dissected out and subjected to detailed study. The percentage somatic growth which was high for control shrimps in contrast to the hormone treated animals have more ovarian growth, suggests an antagonism between these two energy demanding processes.
The failure in producing an external visible change in ovarian development, in comparison to eyestalk ablation (ESA) treatment may be due to the existence of the gonad inhibiting hormone (GIH) produced in the eyestalk which would have blocked / suppressed further development of ovary. It is likely that if a GIH inhibitor is also administered along with steroid hormones or tocopherol the stimulus given by them may result in full ovarian development in penaeid shrimp.
| Item type | Current location | Call number | Status | Date due | Barcode |
|---|---|---|---|---|---|
Theses
|
KAU Central Library, Thrissur Theses | 639.2 JOH/IN (Browse shelf) | Available | 170933 |
MSc
The ovarian maturation and spawning of broodstock shrimp play a major role in the development of commercial level seed production of this valuable crustacean. The already proven technology of induced maturation of penaeids in capativity viz. Unilateral Eyestalk Ablation(UESA) has many negative effects which prompted scientists to explore alternative ways of induced maturation like hormone administration. The hormone progesterone alone and in combination with tocopherol were tested for their effectiveness in bringing about ovary maturation and spawning in pond reared penaeus indicus.
Progesterone and tocopherol dissolved in ethyl alcohol were injected at three test concentrations of 0.05 ug, 0.10ug and 0.20ug/g body weight . For the preparation of combination test solution the dissolved progesterone and tocopherol were mixed in the ratio of 7:3(V/V). Control shrimps were given alcohol treatment at respective concentrations.
The results of the experiment conducted for a period of after 45 days, revealed that the overall survival is not affected (89%) and that progesterone singly or in combination with tocopherol is capable of giving stimulation for ovarian development in P.Indicus Though there was not much observable difference in ovarian development by external viewing, both progesterone alone and in combination with tocopherol at higher dosages have yielded significant GSI difference and ovarian growth, compared with the ethanol treated controls, when the ovaries were dissected out and subjected to detailed study. The percentage somatic growth which was high for control shrimps in contrast to the hormone treated animals have more ovarian growth, suggests an antagonism between these two energy demanding processes.
The failure in producing an external visible change in ovarian development, in comparison to eyestalk ablation (ESA) treatment may be due to the existence of the gonad inhibiting hormone (GIH) produced in the eyestalk which would have blocked / suppressed further development of ovary. It is likely that if a GIH inhibitor is also administered along with steroid hormones or tocopherol the stimulus given by them may result in full ovarian development in penaeid shrimp.
There are no comments for this item.