Cytotoxic and Clastogenic Effects of some Insecticides in Allium cepa, L
By: Jayaprakash Naik B.
Contributor(s): Vijayakumar N K (Guide).
Material type:
BookPublisher: Vellanikkara Department of Agricultural Botany, College of Horticulture 1983DDC classification: 630.28 Online resources: Click here to access online | Click here to access online Dissertation note: MSc Abstract: In the present investigation, the cytotoxic and clastogenic effects of three very commonly used insecticides, namely, aldrin, carbofuran and phorate were tested in Allium cepa, L., a test system.
Four concentrations of each insecticides were used for the study. These concentrations were fixed taking into consideration of their field dose of application in insect control. The treatment periods fixed were 12, 24 and 48 hours. The mitotic index was computed from 4000 cells and indices of each division phases were also computed. The chromosome abnormalities were scored from about 100 to 150 cells of each phases in each treatments.
Aldrin was found to be drastically mitodepressive compared to carbofuran and phorate. While phorate increased mitotic index in the lowest dose, carbofuran showed only marginal reduction. However, both the compounds reduced mitotic index in higher doses and the field doses. Aldrin exhibited a dose and period responsiveness, while carbofuran and phorate could not with regard to mitotic index.
The study also revealed that aldrin is an effective toxicant on both genetic material and proteins. The various chromosome abnormalities noticed were stickiness, bridges, laggards, blurred chromosome borders, chromatin bridge, micronuclei, non-orientation of metaphase, precocious movement in anaphase, chromatin bodies, irregular anaphase, star metaphase, strays, beaked nuclei, break, C-metaphase and unequal nuclei in their decreasing order of occurrence. Unlike aldrin, carbofuran and phorate could not induce anomalies to a significant level in the lowest doses tried, 0.0075 and 0.02 per cent respectively. Chromosome bridge being the most frequent abnormality found in carbofuran, which was followed by stickiness and laggards. The frequency of abnormalities found in field and higher doses were more or less same. Phorate, on the other hand showed linear relationship in inducing chromotoxicities with respect to concentrations and period of treatment. The major types of abnormalities recorded were bridges, breaks and stickiness.
The results showed that all the insecticides tried were capable of affecting the genetic material as well as protein, but to different degrees depending on concentrations. It can be tentatively concluded that they cannot be considered completely safe at the field dose of application on the cellular constituents of the organism. The results call for extensive testing of these chemicals in other test systems also.
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KAU Central Library, Thrissur Theses | 630.28 JAY/CY (Browse shelf) | Available | 171026 |
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MSc
In the present investigation, the cytotoxic and clastogenic effects of three very commonly used insecticides, namely, aldrin, carbofuran and phorate were tested in Allium cepa, L., a test system.
Four concentrations of each insecticides were used for the study. These concentrations were fixed taking into consideration of their field dose of application in insect control. The treatment periods fixed were 12, 24 and 48 hours. The mitotic index was computed from 4000 cells and indices of each division phases were also computed. The chromosome abnormalities were scored from about 100 to 150 cells of each phases in each treatments.
Aldrin was found to be drastically mitodepressive compared to carbofuran and phorate. While phorate increased mitotic index in the lowest dose, carbofuran showed only marginal reduction. However, both the compounds reduced mitotic index in higher doses and the field doses. Aldrin exhibited a dose and period responsiveness, while carbofuran and phorate could not with regard to mitotic index.
The study also revealed that aldrin is an effective toxicant on both genetic material and proteins. The various chromosome abnormalities noticed were stickiness, bridges, laggards, blurred chromosome borders, chromatin bridge, micronuclei, non-orientation of metaphase, precocious movement in anaphase, chromatin bodies, irregular anaphase, star metaphase, strays, beaked nuclei, break, C-metaphase and unequal nuclei in their decreasing order of occurrence. Unlike aldrin, carbofuran and phorate could not induce anomalies to a significant level in the lowest doses tried, 0.0075 and 0.02 per cent respectively. Chromosome bridge being the most frequent abnormality found in carbofuran, which was followed by stickiness and laggards. The frequency of abnormalities found in field and higher doses were more or less same. Phorate, on the other hand showed linear relationship in inducing chromotoxicities with respect to concentrations and period of treatment. The major types of abnormalities recorded were bridges, breaks and stickiness.
The results showed that all the insecticides tried were capable of affecting the genetic material as well as protein, but to different degrees depending on concentrations. It can be tentatively concluded that they cannot be considered completely safe at the field dose of application on the cellular constituents of the organism. The results call for extensive testing of these chemicals in other test systems also.
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