Biological Effects of Gamma Rays and EMS in the M1 Generation of Red Gram(Cajanus cajan L.)
By: Jayanthi S.
Contributor(s): Narayanan Namboothiri K M (Guide).
Material type:
BookPublisher: Vellanikkara Department of Agricultural Botany, College of Horticulture 1986DDC classification: 630.28 Online resources: Click here to access online | Click here to access online Dissertation note: PG Abstract: The studies reported herein were undertaken in the Department of Agricultural Botany, College of Horticulture, Vellanikkara during the period 1983-'85.
Seeds of SA_1 variety of red gram (Cajanus cajan L.) were subjected to induced mutagenesis using five doses of gamma rays (10, 20, 30, 40 and 50 krad) and five doses of EMS (0.3%, 0.4%, 0.5%, 0.6% and 0.7%) and their biological effects on the M1 generation were studied.
In the preliminary laboratory test it was found that the two presoaking times viz., 2 hours an d4 hours did not differ significantly. Same was the case with the two durations of chemical treatments viz., 6 hours and 8 hours. The three concentrations of the chemical viz., 0.5%, 0.75% and 1% tried did differ significantly. The LD50 value was obtained as 0.7%.
Lower doses of both gamma rays and EMS stimulated seed germination. No dose dependance on the percentage of germination was noticed in the case of gamma irradiation while in the case of EMS, germination percentage proportionately decreased with increasing concentrations of the chemical. Gamma ray treated seed took longer time for germination compared to those treated with EMS.
Reduction was observed in the survival percentage of seedling with increase in doses of gamma rays and EMS.
Root length, shoot length and plant height were reduced by gamma rays and EMS, the rate of reduction being directly proportional to the increase in dose.
Pollen and seed fertilities decreased linearly with increase in doses of both gamma rays and EMS. Among the two mutagens, EMS was more effective in reducing pollen and seed fertilities.
Chlorophyll chimeras, in very low frequencies were observed only in the EMS treated population. Morphological variations noticed included dwarf plants, plants with crinkled leaves, those with reduced canopy size, those with prolonged as well as reduced flowering duration, those with lesser number of flowers and fruits. These morphological variations were restricted to EMS treated populations.
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Theses
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KAU Central Library, Thrissur Theses | 630.28 JAY/BI (Browse shelf) | Available | 171083 |
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PG
The studies reported herein were undertaken in the Department of Agricultural Botany, College of Horticulture, Vellanikkara during the period 1983-'85.
Seeds of SA_1 variety of red gram (Cajanus cajan L.) were subjected to induced mutagenesis using five doses of gamma rays (10, 20, 30, 40 and 50 krad) and five doses of EMS (0.3%, 0.4%, 0.5%, 0.6% and 0.7%) and their biological effects on the M1 generation were studied.
In the preliminary laboratory test it was found that the two presoaking times viz., 2 hours an d4 hours did not differ significantly. Same was the case with the two durations of chemical treatments viz., 6 hours and 8 hours. The three concentrations of the chemical viz., 0.5%, 0.75% and 1% tried did differ significantly. The LD50 value was obtained as 0.7%.
Lower doses of both gamma rays and EMS stimulated seed germination. No dose dependance on the percentage of germination was noticed in the case of gamma irradiation while in the case of EMS, germination percentage proportionately decreased with increasing concentrations of the chemical. Gamma ray treated seed took longer time for germination compared to those treated with EMS.
Reduction was observed in the survival percentage of seedling with increase in doses of gamma rays and EMS.
Root length, shoot length and plant height were reduced by gamma rays and EMS, the rate of reduction being directly proportional to the increase in dose.
Pollen and seed fertilities decreased linearly with increase in doses of both gamma rays and EMS. Among the two mutagens, EMS was more effective in reducing pollen and seed fertilities.
Chlorophyll chimeras, in very low frequencies were observed only in the EMS treated population. Morphological variations noticed included dwarf plants, plants with crinkled leaves, those with reduced canopy size, those with prolonged as well as reduced flowering duration, those with lesser number of flowers and fruits. These morphological variations were restricted to EMS treated populations.
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