Anti-Inflammatory and Antinociceptive Effects of Withania Somnifera and Catharanthus Roseus in Rats
By: Arivuchelvan A.
Contributor(s): Joy A D (Guide).
Material type:
BookPublisher: Mannuthy Department of Pharmacology and Toxicology, College of Veterinary and Animal Sciences 1997DDC classification: 636.089 5 Online resources: Click here to access online Dissertation note: MSc Abstract: The present study was undertaken with the objective of
determining the anti-inflammatory and antinociceptive effects
of withania somnifera and Catharanthus roseus. Alcoholic
extract of both the plants were used for the study and the
effect produced by the above plants were compared with that of
the known non-steroidal anti-inflammatory drug namely,
diclofenac sodium which served as the positive control drug.
To assess the anti-inflammatory effect two methods
namely, cotton pellet and carrageenin induced paw oedema were
adopted. In cotton pellet method five groups of eight rats
each were used per plant. First group was kept as a control,
which received five per cent gum acacia only. IInd, IIIrd and
IVth group received 200, 400, 600 mg/kg alcoholic extract of
C. roseus Vth group served as the positive control which
received diclofenac sodium 3 mg/kg dose level. All the drugs
were administered orally. C. roseus produced significant anti
granuloma activity when compared to control group. Higher
activity was produced by 600 mg/kg body weight extract (35.88
per cent anti-inflammatory activity). For W. somnifera also
same experimental design was adopted with dose rates of 750,
1000, 1500 mg/kg body weight. W. somnifera produced dose
dependent antigranuloma activity. Higher dose (1500 mg/kg
body weight) produced more antigranuloma activity (53.92
per cent) which was comparable to the antigranuloma activity
of diclofenac sodium. Haematological parameters before and
after treatment showed no significant changes for both the
plants.
In carrageenin induced paw oedema method al so five groups
of eight rats each were used per plant. All the three doses
of extract and reference drug were given thirty minutes prior
to the carrageenin injection and the paw thickness was
recorded three hour after injection. c. roseus produced
significant antioedema activity in this model. Higher dose
(600 mg/kg) produced equipotent effect compared to diclofenac
sodium 3 mg/kg. W. somnifera also produced dose dependent
anti oedema activity. Extract at the dose rate of 750, 1000,
1500 mg/kg produced 19.4, 35.23, 44.62 per cent antioedema
activity respectively. But the reference drug diclofenac
sodium produced higher antioedema activity.
For evaluating antinociceptive effect of C. roseus and
W. somnifera, seven groups of eight animal each were used.
All the dose rates of both the plant extracts were compared
with diclofenac sodium for a period of two hours showed no
significant analgesic effect.
| Item type | Current location | Call number | Status | Date due | Barcode |
|---|---|---|---|---|---|
Theses
|
KAU Central Library, Thrissur Theses | 636.089 5 ARI/AN (Browse shelf) | Available | 171199 |
MSc
The present study was undertaken with the objective of
determining the anti-inflammatory and antinociceptive effects
of withania somnifera and Catharanthus roseus. Alcoholic
extract of both the plants were used for the study and the
effect produced by the above plants were compared with that of
the known non-steroidal anti-inflammatory drug namely,
diclofenac sodium which served as the positive control drug.
To assess the anti-inflammatory effect two methods
namely, cotton pellet and carrageenin induced paw oedema were
adopted. In cotton pellet method five groups of eight rats
each were used per plant. First group was kept as a control,
which received five per cent gum acacia only. IInd, IIIrd and
IVth group received 200, 400, 600 mg/kg alcoholic extract of
C. roseus Vth group served as the positive control which
received diclofenac sodium 3 mg/kg dose level. All the drugs
were administered orally. C. roseus produced significant anti
granuloma activity when compared to control group. Higher
activity was produced by 600 mg/kg body weight extract (35.88
per cent anti-inflammatory activity). For W. somnifera also
same experimental design was adopted with dose rates of 750,
1000, 1500 mg/kg body weight. W. somnifera produced dose
dependent antigranuloma activity. Higher dose (1500 mg/kg
body weight) produced more antigranuloma activity (53.92
per cent) which was comparable to the antigranuloma activity
of diclofenac sodium. Haematological parameters before and
after treatment showed no significant changes for both the
plants.
In carrageenin induced paw oedema method al so five groups
of eight rats each were used per plant. All the three doses
of extract and reference drug were given thirty minutes prior
to the carrageenin injection and the paw thickness was
recorded three hour after injection. c. roseus produced
significant antioedema activity in this model. Higher dose
(600 mg/kg) produced equipotent effect compared to diclofenac
sodium 3 mg/kg. W. somnifera also produced dose dependent
anti oedema activity. Extract at the dose rate of 750, 1000,
1500 mg/kg produced 19.4, 35.23, 44.62 per cent antioedema
activity respectively. But the reference drug diclofenac
sodium produced higher antioedema activity.
For evaluating antinociceptive effect of C. roseus and
W. somnifera, seven groups of eight animal each were used.
All the dose rates of both the plant extracts were compared
with diclofenac sodium for a period of two hours showed no
significant analgesic effect.
There are no comments for this item.