Comparative efficacy of different antigenic preparations from pasteurella multocida for detection of antibodies by enzyme immuno assay
By: Rinita Sing.
Contributor(s): Jayaprakasan V (Guide).
Material type:
BookPublisher: Mannuthy Department of Microbiology, College of Veterinary and Animal Sciences 1997DDC classification: 636.089 6 Online resources: Click here to access online | Click here to access online Dissertation note: MSc Abstract: In this study IHA, plate ELISA and DIA were employed to
monitor antibody from ducks vaccinated with three different types of
vaccine ( bacterin, bacterin with adjuvant and sonicate adjuvanated
vaccine) prepared from P. multocida. Three different type of antigens
viz., Crude capsular extract (CCE), Potassium thiocyanate extract (
KSCN) and sonicated antigen were used to coat NCM/ microtitre plate
or for sensitization of SRBC.
Forty ducklings of four week age were used for
immunization. They were divided into four groups each group
comprising of ten ducklings. Groups one, two and three were
vaccinated with bacterin, bacterin with adjuvant and sonicate
adjuvanated vaccine, respectively. Antibody was monitored upto 35th
day post vaccination by IHA, Plate ELISA and DIA, employing CCE,
KSCN and sonicated antigen.
Group one ( vaccinated with bacterin) gave a higher mean
titre value followed by IHA and plate ELISA, irrespective of the type of
antigen employed, followed by group three ( birds vaccinated with
sonicate adjuvanated vaccine) and group two ( birds vaccinated with
bacterin with adjuvant ). Irrespective of the antigens employed in the
tests, plate ELISA gave the highest sensitivity ( cent per cent) followed
by IHA and DIA, whereas the highest specificity was observed by DIA,
over IHA and plate ELISA.
When the comparison was made between antigens a high
mean titre value was obtained with sonicated antigen, followed by
KSCN extract and CCE. Crude capsular extract antigen gave cent per
cent specificity by all the tests, while the other two antigens gave a low
percentage of specificity. As the immunological test with highest
specificity is the one preferred, CCE was shown to be superior over
KSCN extract and sonicated antigen in IHA, plate ELISA and DIA for
the detection of specific antibodies against P. multocida in ducks.
| Item type | Current location | Call number | Status | Date due | Barcode |
|---|---|---|---|---|---|
Theses
|
KAU Central Library, Thrissur Theses | 636.089 6 RIN/CO (Browse shelf) | Available | 171276 |
MSc
In this study IHA, plate ELISA and DIA were employed to
monitor antibody from ducks vaccinated with three different types of
vaccine ( bacterin, bacterin with adjuvant and sonicate adjuvanated
vaccine) prepared from P. multocida. Three different type of antigens
viz., Crude capsular extract (CCE), Potassium thiocyanate extract (
KSCN) and sonicated antigen were used to coat NCM/ microtitre plate
or for sensitization of SRBC.
Forty ducklings of four week age were used for
immunization. They were divided into four groups each group
comprising of ten ducklings. Groups one, two and three were
vaccinated with bacterin, bacterin with adjuvant and sonicate
adjuvanated vaccine, respectively. Antibody was monitored upto 35th
day post vaccination by IHA, Plate ELISA and DIA, employing CCE,
KSCN and sonicated antigen.
Group one ( vaccinated with bacterin) gave a higher mean
titre value followed by IHA and plate ELISA, irrespective of the type of
antigen employed, followed by group three ( birds vaccinated with
sonicate adjuvanated vaccine) and group two ( birds vaccinated with
bacterin with adjuvant ). Irrespective of the antigens employed in the
tests, plate ELISA gave the highest sensitivity ( cent per cent) followed
by IHA and DIA, whereas the highest specificity was observed by DIA,
over IHA and plate ELISA.
When the comparison was made between antigens a high
mean titre value was obtained with sonicated antigen, followed by
KSCN extract and CCE. Crude capsular extract antigen gave cent per
cent specificity by all the tests, while the other two antigens gave a low
percentage of specificity. As the immunological test with highest
specificity is the one preferred, CCE was shown to be superior over
KSCN extract and sonicated antigen in IHA, plate ELISA and DIA for
the detection of specific antibodies against P. multocida in ducks.
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