Genetic Studies on Serum Alkaline Phosphatase and Haemoglobin in two Strains of White Leghorn
By: Sankaralingam S.
Contributor(s): Unni A K K (Guide).
Material type:
BookPublisher: Mannuthy Department of Poultry Science, College of Veterinary and Animal Sciences 1997DDC classification: 636.5 Online resources: Click here to access online | Click here to access online Dissertation note: MSc Abstract: The present investigation was undertaken to identify the Serum Alkaline
Phosphatase (SAP) and haemoglobin (Hb) polymorphism and also aimed to
find their association with production, egg quality and reproduction. Four
hundred and ninety seven hens of two different strains of White Leghorn Viz.
IWP (168 and 100) and IWN (129 and 100) belonging to S15 and S16
generations, respectively were typed by Horizontal Polyacrylamide Gel
Electrophoresis (PAGE). Two phenotypes, Fast and Slow were determined.
Higher frequencies of Fast phenotype were observed in IWP strain (0.66 and
0.58) than IWN strain (0.15 and 0.24) in both S15 and S16 generations,
respectively. Hundred hens each of IWP and IWN strains were tested for
haemoglobin polymorphism with Agar gel electrophoresis, which revealed no
polymorphism.
Two alleles namely AkpF and Akps with two phenotypes Fast and Slow
were identified as SAP locus. AkpF allele had the frequency of 0.42 and 0.35
in IWP strain and 0.08 and 0.13 in IWN strain of S15 and S16 generations,
respectively. Different matings between Fast and Slow moving types revealed
that the Fast band is determined by an autosomal completely dominant gene
over Slow band bird. The mean SAP level for the F and S birds revealed that
the SAP activity was more in F type birds (97.54 and 88.96 KA units per 100ml
serum) than that of S type birds (93.03 and 68.24 KA units per 100ml serum)
in both IWP and IWN strains respectively.
The association between SAP types and egg quality traits revealed no
significant difference between them except shell thickness. The eggs from F
type birds had significantly higher shell thickness than S type in IWP and IWN
strains of White Leghorn. Correlation between different SAP type matings and
reproduction traits revealed that the fertility of F x F and F x S matings were
highest followed by S x Sand S x F. The hatchability (on fertile egg set) was
high in F x F followed by F x Sand S x F, the least hatchability was observed
in S x S cross.
The findings tend to conclude that the birds having gene AkpF perform
better in respect of egg production, egg quality and reproduction traits. The
egg production at the later stage of life was found to be high in F type birds
and hence it is desirable to follow selection on extended testing periods than
relying on 40 weeks egg records.
| Item type | Current location | Call number | Status | Date due | Barcode |
|---|---|---|---|---|---|
Theses
|
KAU Central Library, Thrissur Theses | 636.5 SAN/GE (Browse shelf) | Available | 171284 |
MSc
The present investigation was undertaken to identify the Serum Alkaline
Phosphatase (SAP) and haemoglobin (Hb) polymorphism and also aimed to
find their association with production, egg quality and reproduction. Four
hundred and ninety seven hens of two different strains of White Leghorn Viz.
IWP (168 and 100) and IWN (129 and 100) belonging to S15 and S16
generations, respectively were typed by Horizontal Polyacrylamide Gel
Electrophoresis (PAGE). Two phenotypes, Fast and Slow were determined.
Higher frequencies of Fast phenotype were observed in IWP strain (0.66 and
0.58) than IWN strain (0.15 and 0.24) in both S15 and S16 generations,
respectively. Hundred hens each of IWP and IWN strains were tested for
haemoglobin polymorphism with Agar gel electrophoresis, which revealed no
polymorphism.
Two alleles namely AkpF and Akps with two phenotypes Fast and Slow
were identified as SAP locus. AkpF allele had the frequency of 0.42 and 0.35
in IWP strain and 0.08 and 0.13 in IWN strain of S15 and S16 generations,
respectively. Different matings between Fast and Slow moving types revealed
that the Fast band is determined by an autosomal completely dominant gene
over Slow band bird. The mean SAP level for the F and S birds revealed that
the SAP activity was more in F type birds (97.54 and 88.96 KA units per 100ml
serum) than that of S type birds (93.03 and 68.24 KA units per 100ml serum)
in both IWP and IWN strains respectively.
The association between SAP types and egg quality traits revealed no
significant difference between them except shell thickness. The eggs from F
type birds had significantly higher shell thickness than S type in IWP and IWN
strains of White Leghorn. Correlation between different SAP type matings and
reproduction traits revealed that the fertility of F x F and F x S matings were
highest followed by S x Sand S x F. The hatchability (on fertile egg set) was
high in F x F followed by F x Sand S x F, the least hatchability was observed
in S x S cross.
The findings tend to conclude that the birds having gene AkpF perform
better in respect of egg production, egg quality and reproduction traits. The
egg production at the later stage of life was found to be high in F type birds
and hence it is desirable to follow selection on extended testing periods than
relying on 40 weeks egg records.
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