Super ovulation and Embryo Recovery in Rabbits
By: Satheeshkumar S.
Contributor(s): Suresh Nair S P (Guide).
Material type:
BookPublisher: Mannuthy Department of Animal Reproduction, College of Veterinary and Animal Sciences 1997DDC classification: 636.082 Online resources: Click here to access online | Click here to access online Dissertation note: MVSc Abstract: Superovulation was induced in Newzealand White and Soviet
Chinchilla breeds of rabbits by administration of a single
dose of 150 IU PMSG followed by double mating at induced cycle
and 150 IU HCG soon after second mating to induce ovulation.
The onset and intensity of oestrum, number of ovulations,
embryo recovery and quality of embryos were studied and
compared with those of the controls of the respective breeds.
The mean interval from PMSG administration to onset of
oestrus in both the breeds was 56.0 ± 5.1 h. It was further
observed that most of the treated animals showed intense
oestrum when compared to controls.
The ovulation rate based on the number of corporalutea in
control animals of Newzealand White breed was 4.7 ± 1.2 as
against 22.0 ± 1.35 in the treated group. There was
significant difference (P<0.0l) in the ovulation rate between
the groups. The percentages of embryo recovery, fertile
embryos and transferrable embryos in the control group were
47.22, 86.7 and 92.3 while those of the treatment group were
31.67, 100 and 87.8 respectively. There was no statistically
significant difference between the groups.
While the control animals in Soviet Chinchilla breed had
an ovulation rate of 6.7 ± 0.65, the treated rabbits showed a
higher ovulation rate of 20.0 ± 3.2. There was significantly
higher ovulation rate (P<0.01) in treated group when compared
to controls. The embryo recovery rate, fertilized embryos and
transferrable embryos in the control group were 53.33 per
cent, 77.8 per cent and 93.8 per cent respectively. The
corresponding values in the treatment group were 36.27 per
cent, 69.82 per cent and 93.1 per cent respectively. There
was no significant difference between the groups. No breed
influence on the above parameters could also be noticed in
this study
It may be concluded that superovulation could. be
successfully induced in Newzealand White and Soviet Chinchilla
breeds of rabbits with single dose of 150 IU PMSG, followed by
150 IU HCG soon after second mating. Even though there was
superovulation, the embryo recovery rate was comparatively
lower in the treated group probably on account of an altered
oestrogen-progesterone profile interfering with the transport
of the zygote, however the fertilization rate and the quality
of the embryos were unaffected with the superovulation
treatment.
| Item type | Current location | Call number | Status | Date due | Barcode |
|---|---|---|---|---|---|
Theses
|
KAU Central Library, Thrissur Theses | 636.082 SAT/SU (Browse shelf) | Available | 171287 |
MVSc
Superovulation was induced in Newzealand White and Soviet
Chinchilla breeds of rabbits by administration of a single
dose of 150 IU PMSG followed by double mating at induced cycle
and 150 IU HCG soon after second mating to induce ovulation.
The onset and intensity of oestrum, number of ovulations,
embryo recovery and quality of embryos were studied and
compared with those of the controls of the respective breeds.
The mean interval from PMSG administration to onset of
oestrus in both the breeds was 56.0 ± 5.1 h. It was further
observed that most of the treated animals showed intense
oestrum when compared to controls.
The ovulation rate based on the number of corporalutea in
control animals of Newzealand White breed was 4.7 ± 1.2 as
against 22.0 ± 1.35 in the treated group. There was
significant difference (P<0.0l) in the ovulation rate between
the groups. The percentages of embryo recovery, fertile
embryos and transferrable embryos in the control group were
47.22, 86.7 and 92.3 while those of the treatment group were
31.67, 100 and 87.8 respectively. There was no statistically
significant difference between the groups.
While the control animals in Soviet Chinchilla breed had
an ovulation rate of 6.7 ± 0.65, the treated rabbits showed a
higher ovulation rate of 20.0 ± 3.2. There was significantly
higher ovulation rate (P<0.01) in treated group when compared
to controls. The embryo recovery rate, fertilized embryos and
transferrable embryos in the control group were 53.33 per
cent, 77.8 per cent and 93.8 per cent respectively. The
corresponding values in the treatment group were 36.27 per
cent, 69.82 per cent and 93.1 per cent respectively. There
was no significant difference between the groups. No breed
influence on the above parameters could also be noticed in
this study
It may be concluded that superovulation could. be
successfully induced in Newzealand White and Soviet Chinchilla
breeds of rabbits with single dose of 150 IU PMSG, followed by
150 IU HCG soon after second mating. Even though there was
superovulation, the embryo recovery rate was comparatively
lower in the treated group probably on account of an altered
oestrogen-progesterone profile interfering with the transport
of the zygote, however the fertilization rate and the quality
of the embryos were unaffected with the superovulation
treatment.
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